Crops ›› 2025, Vol. 41 ›› Issue (1): 243-249.doi: 10.16035/j.issn.1001-7283.2025.01.031

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Identification of Osmotic Stress Resistance and Analysis of Related Agronomic Traits of 71 Winter Wheat Seedlings

Yang Dandan(), Han Xue, Kong Xinxin, Zhao Guoxuan, Su Yazhong, Zhao Pengfei, Jin Jianmeng, Zhao Guojian()   

  1. Kaifeng Academy of Agriculture and Forestry Sciences, Kaifeng 475004, Henan, China
  • Received:2023-12-29 Revised:2024-03-26 Online:2025-02-15 Published:2025-02-12

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Abstract:

In order to understand the osmotic stress resistance of various winter wheat varieties (lines), screen effective drought resistance indexes at the seedling stage, and shorten the time of screening drought-resistant wheat varieties, 71 winter wheat varieties (lines) were used as experimental materials. Field drought stress was simulated by using PEG-6000, and eight seedling stage indexes were determined under drought stress and control environment, including total leaf number, penultimate leaf width, penultimate leaf length, penultimate leaf chlorophyll content, plant height, root length, underground fresh weight, and aboveground fresh weight. The osmotic-stress resistance of wheat varieties (lines) was comprehensively evaluated by means of membership function method, cluster analysis and principal component analysis. The results showed that the variation coefficient of each index in the CK were 9.77%-68.26%, and those in the stress group were 12.51%-69.15%, and the variation coefficients of underground fresh weights were the largest in both groups. The underground fresh weight of stress group was lower than that of CK, and the other indexes varied slightly. Under the two treatments, the correlation changes of seedling traits were different, and there was interaction among the traits. The comprehensive drought resistance coefficient (D) was introduced to evaluate the drought resistance at seedling stage. Principal component analysis was carried out on the subordinate function values of drought- resistance coefficient of each characteristic, and eight characteristics were summarized into four components, with a cumulative contribution rate of 73.204%. Cluster analysis divided 71 wheat materials into four groups: high resistance, drought resistance, moderate resistance and drought sensitivity, of which eight materials were above the drought resistance level.

Key words: Winter wheat, Osmotic stress resistance, Drought resistance, Cluster analysis

Table 1

Test wheat varieties (lines)"

编号
Number		 品种（系）
Variety (line)		 编号
Number		 品种（系）
Variety (line)
C1 新麦45 C37 开麦1801
C2 新麦38 C38 开麦1802
C3 郑麦159 C39 开麦1803
C4 豫农908 C40 开麦1805
C5 泛麦28 C41 开麦1915
C6 豫农922 C42 开麦1916
C7 豫农536 C43 开麦1917
C8 郑麦024 C44 开麦1918
C9 郑麦369 C45 开麦18
C10 郑麦366 C46 开麦21
C11 郑麦158 C47 开麦20
C12 郑麦136 C48 开麦1606
C13 郑麦918 C49 开麦26
C14 周麦27 C50 开麦1502
C15 周麦30 C51 开麦1502矮系
C16 新麦26 C52 开麦201
C17 新麦28 C53 开麦202
C18 郑麦103 C54 开麦203
C19 郑麦119 C55 开麦205
C20 郑麦379 C56 开麦206
C21 郑麦583 C57 开麦207
C22 中麦518 C58 开麦208
C23 万丰269 C59 开麦209
C24 郑麦023 C60 谷丰8号
C25 郑麦215 C61 周麦9号
C26 郑麦213 C62 济麦22
C27 周麦22 C63 天民339
C28 良星66 C64 平安106
C29 良星99 C65 新麦60
C30 百农307 C66 平安21
C31 开麦22 C67 郑麦6183
C32 开麦1701 C68 平安17
C33 开麦1702 C69 金粒826
C34 开麦1703 C70 洛旱7号
C35 开麦1705 C71 周麦36
C36 开麦1706

Table 2

Description and statistics of eight seedling indexes of test wheat varieties (lines)"

处理Treatment 指标Index 最小值Min. 最大值Max. 均值Mean 标准差SD 变异系数CV (%)
对照组CK 总叶片数 4.0 6.0 5.16 0.50 9.77
倒二叶叶宽 0.1 0.8 0.34 0.12 34.36
倒二叶叶长 0.3 19.8 11.09 1.84 16.63
倒二叶叶绿素含量 2.0 56.4 32.35 9.99 30.90
株高 10.5 30.0 20.42 3.97 19.44
根长 0.5 10.5 5.23 2.21 42.25
地下鲜重 0.01 0.80 0.08 0.07 68.26
地上鲜重 0.04 2.19 0.57 0.31 55.21
胁迫组Stress group 总叶片数 4.0 8.0 5.07 0.64 12.51
倒二叶叶宽 0.2 0.6 0.32 0.08 25.37
倒二叶叶长 4.0 15.3 9.97 1.74 17.47
倒二叶叶绿素含量 4.6 41.5 27.69 6.83 24.67
株高 10.2 27.2 19.83 3.17 15.98
根长 0.9 16.5 4.24 2.20 51.88
地下鲜重 0.02 0.28 0.05 0.03 69.15
地上鲜重 0.09 4.40 0.43 0.27 64.37

Table 3

Correlation analysis of eight seedling indexes of test wheat varieties (lines)"

处理Treatment 指标Index TLN 2nd LW 2nd LL 2nd LCh PH RL SFW
对照组CK 2nd LW -0.04
2nd LL -0.01 0.12
2nd LCh -0.02 0.14 0.02
PH 0.14 0.43*** 0.14 0.32**
RL 0.03 -0.02 -0.17 0.17 -0.01
SFW 0.08 0.34** -0.03 0.35** 0.24* -0.04
RFW 0.16 0.70*** 0.06 0.24* 0.72*** 0.15 0.46***
胁迫组Stress group 2nd LW -0.11
2nd LL 0.14 -0.08
2nd LCh -0.18 -0.07 0.28*
PH 0.30* -0.08 0.25* 0.05
RL -0.03 -0.16 0.14 -0.04 0.04
SFW -0.08 0.33** -0.13 -0.42*** 0.39*** -0.07
RFW 0.18 0.16 0.14 -0.01 0.38** -0.05 0.11

Table 4

Eigenvalues and contribution rates of four principal components"

指标Index 1 2 3 4
特征值Eigenvalue 2.382 1.366 1.102 1.006
贡献率Contribution rate (%) 29.774 17.074 13.781 12.575
累计贡献率
Cumulative contribution rate (%)		 29.774 46.848 60.629 73.204

Table 5

Loading matrix of each component in four principal components"

成分Component TLN 2nd LW 2nd LL 2nd LCh
1 0.151 0.677 -0.664 0.101
2 0.512 -0.419 -0.273 -0.169
3 0.043 0.050 0.238 0.931
4 0.793 0.169 0.469 -0.079
成分Component PH RL SFW RFW
1 0.736 -0.261 0.364 0.841
2 0.446 0.550 -0.563 0.074
3 0.291 -0.076 -0.256 -0.139
4 -0.199 -0.214 0.050 0.187

Table 6

D-values of different cluster groups"

类群
Group		 D值D-value 抗性
Resistance
变幅Range 平均Mean
Ⅰ 0.98 0.98 高抗
Ⅱ 0.59~0.67 0.61 抗
Ⅲ 0.44~0.57 0.49 中抗
Ⅳ 0.32~0.43 0.40 敏感

Fig.1

Cluster map of drought resistance of 71 wheat varieties (lines)"

Table 7

Correlation analysis of D-values and seedling drought resistance coefficients"

指标Index TLN 2nd LW 2nd LL 2nd LCh PH RL SFW RFW
D值D-value 0.103 0.520** -0.176 0.220 0.335** 0.206 0.794** 0.579**

Fig.2

Analysis of difference of each index among drought resistant groups Different lowercase letters indicate significant differences among materials at P < 0.05, only annotate groups with significant differences."

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