Crops ›› 2026, Vol. 42 ›› Issue (1): 118-124.doi: 10.16035/j.issn.1001-7283.2026.01.015

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Effects of Silicon on Stem Rust Resistance and Antioxidant Properties in Oats

Li Yinghao1,2(), Yang Ao1(), Liu Jinghui1(), Tian Lu2, Mi Junzhen1   

  1. 1College of Agriculture, Inner Mongolia Agricultural University / Oat Engineering Research Center of Inner Mongolia Universities / Oat Engineering Laboratory of Inner Mongolia Autonomous Region / Collaborative Innovation Center of Grain Industry of Inner Mongolia Agricultural University, Hohhot 010019, Inner Mongolia, China
    2Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, Inner Mongolia, China
  • Received:2024-09-19 Revised:2024-11-08 Online:2026-02-15 Published:2026-02-10

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Abstract:

To explore the effects of silicon (Si) on the physiological characteristics of oat leaves infected by Puccinia graminis f. sp. avenae and clarify the physiological mechanism of silicon-induced resistance to stem rust, a pot experiment was conducted using ?Bayou 1?, an oat cultivar highly susceptible to stem rust, as the experimental material. Four treatments were established: CK (no silicon, no stem rust pathogen inoculation), +Si-P (silicon application, no stem rust pathogen inoculation), -Si+P (no silicon, stem rust pathogen inoculation), and +Si+P (silicon application, stem rust pathogen inoculation). This study investigated the effects of 1.5 mmol/L silicon application on the disease progression, reactive oxygen species content, and antioxidant enzyme activities of oat leaves after stem rust pathogen infection. The results showed that silicon application effectively delayed the disease development process of oat stem rust, and significantly alleviated disease symptoms. Stem rust pathogen infection led to a rapid increase in O2-. and hydrogen peroxide content in leaves. Simultaneously, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), malondialdehyde (MDA) content, and relative electrical conductivity of leaves increased rapidly, while ascorbate peroxidase (APX) activity decreased rapidly. Within seven days after stem rust pathogen inoculation, silicon application reduced the reactive oxygen species content in leaves, while increasing the activities of SOD, POD, and CAT, and decreasing APX activity and MDA content. Furthermore, silicon application reduced the relative electrical conductivity of leaves within 11 days after inoculation. Silicon application enhanced the antioxidant defense ability of oat leaves, reduced the degree of membrane lipid peroxidation, and enhanced oat resistance to stem rust.

Key words: Silicon, Oat, Stem rust, Reactive oxygen species, Antioxidant enzymes

Fig.1

Schematic diagram of dark treatment culture device"

Fig.2

Effect of silicon application on the resistance to stem rust in oats"

Fig.3

Effects of inoculation with Pga and silicon application on the contents of O2-. and H2O2 in oat leaves"

Fig.4

Effects of inoculation with Pga and silicon application on protective enzyme system of oat leaves"

Fig.5

Effects of inoculation with Pga and silicon application on plasma membrane permeability of oat leaves"

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