Crops ›› 2017, Vol. 33 ›› Issue (3): 60-68.doi: 10.16035/j.issn.1001-7283.2017.03.012

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Effects of Exogenous Salicylic Acid on Cell Ultrastructure of Winter Wheat under Cold Stress in Frigid Region

Liu Lijie1,Zhang Dongxiang1,Jin Zhongmin1,Lin Lidong1,Ren Yanbo1,Zhao Jingtong1,Cang Jing2   

  1. 1 College of Life Science and Agriculture Forestry,Qiqihar University,Qiqihar 161006,Heilongjiang,China
    2 College of Life Science,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
  • Received:2017-03-07 Revised:2017-04-26 Online:2017-06-15 Published:2018-08-26
  • Contact: Jing Cang

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Abstract:

In order to investigate the effects of exogenous salicylic acid (SA) on cell ultrastructure of winter wheat under low temperature, Dongnongdongmai No.1 (the winter wheat cultivar in frigid region) was used as material and 1×10 -4 mol/L SA was sprayed at seedlings growing to three leaves stages using spraying distilled water as control. In the winter natural cooling conditions, tillering nodes and stems were sampled, at 5℃, 0℃, -10℃, and -25℃, respectively,while Malondialdehyde (MDA) content was determined and the ultrastructure of the cells were observed by transmission electron microscopy, and the return rate and the number of tillering nodes were counted. The results showed that exogenous SA alleviated the damage caused by low temperature on membrane system. The cells in the control group appeared slight plasmolysis and slight vacuolization, mitochondrial membrane and nuclear membrane was not clear, but there was no damage to the nucleus only at -25℃. After the application of SA, nuclear damage degree was low, the nuclear and mitochondria membrane were clear, the number of cristae was slightly increased, the number of mitochondria increased in tillering nodes, Only at -25℃, nuclear membrane and mitochondrial membrane were not clear, and they were slightly damaged, but lighter than the control group; The number of plastids and starch grains were increased (above 0℃), and the number of vacuoles in tillering nodes was significantly more than that in the control group. The return rate and tillering nodes number were increased after reviving by exogenous SA. This showed that SA pretreatment could enhance the stability of the cell structure at low temperature, so as to improve the adaptability to low temperature of winter wheat in cold region, from the view of cytology it was confirmed the application of suitable concentration of SA could enhance the cold resistance of winter wheat in cold area.

Key words: Winter wheat cultivar in frigid region, Exogenous salicylic acid, Cell ultrastructure

Fig.1

Change of maximum, minimum, and average temperature during the experiment"

Fig.2

Effects of SA on MDA content of tillering nodesof winter wheat cultivar in frigid region"

Fig.3

Effects of SA on MDA content of stems of winter wheat cultivar in frigid region"

Fig.4

The ultrastructure of tillering nodes of winter wheat in frigid region in the control group at 5℃N: Nucleus; M: Mitochondria; V: Vacuole; Pr: Proplastid, the same below"

Fig.5

The ultrastructure of tillering nodes of winter wheat in frigid region in SA treatment group at 5℃"

Fig.6

The ultrastructure of stems of winter wheat in frigid region in the control group at 5℃"

Fig.7

The ultrastructure of stems of winter wheat in frigid region in SA treatment group at 5℃"

Fig.8

The ultrastructure of tillering nodes of winter wheat in frigid region in the control group at 0℃"

Fig.9

The ultrastructure of tillering nodes of winter wheat in frigid region in SA treatment group at 0℃"

Fig.10

The ultrastructure of stems of winter wheat in frigid region in the control group at 0℃"

Fig.11

The ultrastructure of stems of winter wheat in frigid region in SA treatment group at 0℃"

Fig.12

The ultrastructure of tillering nodes of winter wheat in frigid region in the control group at -10℃"

Fig.13

The ultrastructure of tillering nodes of winter wheat in frigid region in SA treatment group at -10℃"

Fig.14

The ultrastructure of stems of winter wheat in frigid region in the control group at -10℃"

Fig.15

The ultrastructure of stems of winter wheat in frigid region in SA treatment group at -10℃"

Fig.16

The ultrastructure of tillering nodes of winter wheat in frigid region in the control group at -25℃"

Fig.17

The ultrastructure of tillering nodes of winter wheat in frigid region in SA treatment group at -25℃"

Fig.18

The ultrastructure of stems of winter wheat in frigid region in the control group at -25℃"

Fig.19

The ultrastructure of stems of winter wheat in frigid region in SA treatment group at -25℃"

Table 1

Investigation of reviving rate"

处理
Treatment		 分蘖节数Number of tillering nodes 返青率(%)
Reviving rate
越冬前Before winter 返青后 After reviving
对照(CK) 3 3 85
SA 3 8 91
[1] Mohammadreza A, Aghdam M S . Impact of salicylic acid on post-harvest physiology of horticultural crops. Trends in Food Science and Technology, 2010,21(9):502-509.
doi: 10.1016/j.tifs.2010.07.009
[2] Yang J H, Gao Y, Li Y M , et al. Salicylic acid-induced enhancement of cold tolerance through activation of antioxidative capacity in watermelon. Scientia Horticulturae, 2008,118(3):200-205.
doi: 10.1016/j.scienta.2008.06.015
[3] 刘国强, 吴锦程, 朱颖 , 等. 水杨酸对低温胁迫下枇杷幼果若干生理生化指标的影响. 热带作物学报, 2009,30(3):254-258.
doi: 10.3969/j.issn.1000-2561.2009.03.002
[4] 周利民, 陈惠萍 . 水杨酸对冷胁迫下香蕉幼苗抗冷性的效应. 亚热带植物科学, 2009,38(1):19-22.
doi: 10.3969/j.issn.1009-7791.2009.01.005
[5] 邓世媛, 陈建军, 罗福命 , 等. 外源水杨酸对低温胁迫下烤烟抗氧化代谢的影响.烟草科技, 2012(2):71-74.
[6] 朱璇, 侯媛媛, 贾燕 , 等. 水杨酸处理对冷藏杏果实细胞超微结构的影响. 食品科学, 2014,35(14):193-197.
doi: 10.7506/spkx1002-6630-201414038
[7] 张俊环, 王玉柱, 孙浩元 , 等. 外源SA对低温下杏花器官超微结构的影响. 园艺学报, 2014,41(3):429-436.
[8] 黄儒 . 低温下外源SA对冬小麦东农冬麦1号抗氧化系统的影响. 哈尔滨:东北农业大学, 2014.
[9] 吴冰 . 水杨酸处理对低温下冬小麦蔗糖代谢的影响. 哈尔滨:东北农业大学, 2015.
[10] 王涛, 王晓楠, 王明芳 , 等. 低温下冬小麦水分含量的变化与抗寒性鉴定.作物杂志, 2015(1):61-66.
[11] Dhindsa R S, Dhindsa P P, Thrope T A . Leaf senescence:correlated with increased levels of member permeability and lipid peroxidation,and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany, 1981,3:93-101.
[12] Zhang J H, Huang W D, Liu Y P , et al. Effect of temperature acclimation pretreatment on the ultrastructure of mesophyll cells in young grape plants (Vitis vinifera L.cv.Jingxiu) under crosstemperature stresses. Journal of Integrative Plant Biology, 2005,47(8):959-970.
doi: 10.1111/jipb.2005.47.issue-8
[13] Li D M, Guo Y K, Li Q , et al. The pretreatment of cucumber with methyl jasmonate regulates antioxidant enzyme activities and protects chloroplast and mitochondrial ultrastructure in chilling-stressed leaves. Scientia Horticulturae, 2012,143:135-143.
doi: 10.1016/j.scienta.2012.06.020
[14] Kratsch H A, Wise R R . The ultrastructure of chilling stress. Plant Cell and Environment, 2000,23(4):337-350.
doi: 10.1046/j.1365-3040.2000.00560.x
[15] 付连双, 王晓楠, 王学东 , 等. 低温驯化及封冻后不同抗寒性小麦品种细胞超微结构的比较. 麦类作物学报, 2010,30(1):66-72.
doi: 10.7606/j.issn.1009-1041.2010.01.014
[16] 徐琛 . 外源ABA对低温下冬小麦膜质抗寒性的影响. 哈尔滨:东北农业大学, 2014.
[17] 宋吉仁, 陈超, 王晓楠 , 等. 越冬期抗寒性不同冬小麦品种间生理指标的比较.作物杂志, 2013(4):70-74.
[18] 武维华 . 植物生理学.北京: 科技出版社, 2003: 12-17.
[19] Ishikawa H A . Ultrastructural features of chilling injury:injured cells and the early events during chilling of suspension-cultured mung bean cells. American Journal of Botany, 1996,83(7):825-835.
doi: 10.1002/j.1537-2197.1996.tb12774.x
[20] Ritali Z, Ashworth E N . Response of xylem ray parenchyma cells of supercooling wood tissues to freezing stress:microscopic study. International Journal of Plant Sciences, 1995,156(6):784-792.
doi: 10.1086/297302
[21] 简令成, 荆玉祥, 张宝田 . 植物抗寒性的细胞学研究-小麦进入寒冬时期细胞亚显微结构的变化.植物学报, 1973(1):24-38.
[22] Ledbetter M C, Porter K R . Introduction to the fine structure of plant cells.Spriger-verlag: Berlin-Heidelberg, 1971.
[23] 杜艳 . 低温胁迫下两种七叶树苗木超微结构的比较. 南京林业大学学报, 2007,31(3):111-114.
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