Crops ›› 2021, Vol. 37 ›› Issue (5): 35-42.doi: 10.16035/j.issn.1001-7283.2021.05.006

Previous Articles     Next Articles

Preparations and Comparative Analysis of Photosynthetic Protein Complexes in Different Crops

Wang Ruqing1,2(), Hua Wei1, Liu Jun1()   

  1. 1Oil Crops Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, Hubei, China
    2Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2020-11-17 Revised:2021-01-25 Online:2021-10-15 Published:2021-10-14
  • Contact: Liu Jun E-mail:365339576@qq.com;liujunocr@caas.cn

RichHTML

11

PDF (PC)

606

Abstract:

We performed analysis of the similarities and differences of photosynthetic protein complexes from C3, C4, C3-C4 intermediary plants and polyploid plants by using Blue Native (BN)-PAGE technology. Diploid C3 plants Arabidopsis and tobacco, monocot C3 plant rice, C4 plant maize, white cauliflower, C3-C4 intermediary plant MS, C3 plants rape, soybean and peanut were used as materials. To optimize the BN-PAGE system, we demonstrated that the optimal procedures are as follows. The final solubilization concentration of 1% should be used for the membrane protein non-ionic detergent n-Dodecyl-β-D-Maltopyranoside (DM) and the appropriate treatment time should be 10 minutes in Arabidopsis. This optimized system was then employed to analyze photosynthetic complexes (or thylakoids) in different types of crops. Our datas indicated that the photosynthetic complexes and their key subunits were dramatically distinct in terms of protein abundance and composition. We found that the content of PSII core protein D1 was higher in C4, monocots and polyploid crops compared to C3, dicots and diploid crops, respectively. Oryza sativa had the highest amount of Cyt b6f complex in all the crops. In particular, the core subunit Cyt f of Cyt b6f varied substantially between the different crops. The abundance of PSI core subunit PsaA in Zea mays was remarkably higher relative to the other eight crops, and the contents of C4 plants were higher than that of C3 plants. Monocots contained significantly higher level of ATPase core subunit CF1β than dicots. Taken together, our findings provide reference for further improvement of photosynthetic traits and enhancement of photosynthetic efficiency in crops.

Key words: Photosynthesis, Thylakoid membrane complexes, Blue native polyacrylamide gel electrophoresis (BN-PAGE), Photosynthetic protein, Crops

Fig.1

BN/SDS -PAGE analysis of thylakoid membrane protein complexes in the model plant Arabidopsis a: unstained 1D BN-PAGE, b: stained 1D BN-PAGE, c: thylakoid membrane protein complexes insolated by 2D BN/SDS PAGE"

Fig.2

BN-PAGE analysis of thylakoid membranes in different crops a: unstained 1D BN-PAGE in the nine crops, b: stained 1D BN-PAGE in the nine crops"

Fig.3

BN-PAGE coupled with immunoblotting analyses of thylakoid membranes in different crops"

Fig.4

Immunoblotting analysis of photosynthetic proteins in different crops"

Fig.5

Analysis of thylakoid membrane components in different crops by 2D BN/SDS PAGE"

[1] 朱新广, 熊燕, 阮梅花, 等. 光合作用合成生物学研究现状及未来发展策略. 中国科学院院刊, 2018, 33(11):1239-1248.
[2] Zhu X G, Long S P, Ort D R. Improving photosynthetic efficiency for greater yield. Annual Review of Plant Biology, 2010, 61:235-261.
doi: 10.1146/arplant.2010.61.issue-1
[3] Ort D R, Merchant S S, Alric J, et al. Redesigning photosynthesis to sustainably meet global food and bioenergy demand. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(28):8529-8536.
[4] Bailey-Serres J, Parker J E, Ainsworth E A, et al. Genetic strategies for improving crop yields. Nature, 2019, 575(7781):109-118.
doi: 10.1038/s41586-019-1679-0
[5] 匡廷云, 卢从明. 加强光能高效利用机理研究的战略意义. 科学与社会, 2011, 1(3):23-27.
[6] Chen J H, Chen S T, He N Y, et al. Nuclear-encoded synthesis of the D1 subunit of photosystem II increases photosynthetic efficiency and crop yield. Nature Plants, 2020, 6(5):570-580.
doi: 10.1038/s41477-020-0629-z
[7] 张立新, 彭连伟, 林荣呈, 等. 光合作用研究进展与前景. 中国基础科学, 2016, 18(1):13-20.
[8] 李辉严, 马三梅. C3、C4和C3-C4中间型植物的进化. 植物生理学通讯, 2008, 44(5):1004-1006.
[9] Wittig I, Braun H P, Schägger H. Blue native PAGE. Nature Protocols, 2006, 1(1):418-428.
pmid: 17406264
[10] 芦然. 蓝绿温和胶电泳及其在植物类囊体膜蛋白组研究中的应用. 黑龙江科技信息, 2011(9):64-64.
[11] 李贝贝, 郭进魁, 周云, 等. 一种分析叶绿体类囊体膜色素蛋白复合物的蓝绿温和胶电泳系统. 生物化学与生物物理进展, 2003, 30(4):639-643.
[12] 叶露幻, 郑宝刚, 沈唯军, 等. 水稻剑叶类囊体膜蛋白复合物的蓝绿温和胶电泳分析. 江苏农业科学, 2013, 41(9):42-45.
[13] Järvi S, Suorsa M, Paakkarinen V, et al. Optimized native gel systems for separation of thylakoid protein complexes:novel super-and mega-complexes. Biochemical Journal, 2011, 439(2):207-214.
doi: 10.1042/BJ20102155 pmid: 21707535
[14] Porra R J, Thompson W A, Kriedemann P E. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents:verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta Bioenergetics, 1989, 975(3):384-394.
doi: 10.1016/S0005-2728(89)80347-0
[15] Liu J, Last R L. A land plant-specific thylakoid membrane protein contributes to photosystem II maintenance in Arabidopsis thaliana. The Plant Journal, 2015, 82(5):731-743.
doi: 10.1111/tpj.2015.82.issue-5
[16] Liu J, Last R L. A chloroplast thylakoid lumen protein is required for proper photosynthetic acclimation of plants under fluctuating light environments. Proceedings of the National Academy of Sciences of the United States of America, 2017, 114(38):E8110-E8117.
[17] Liu J, Yang H, Lu Q, et al. PsbP-domain protein1,a nuclear-encoded thylakoid lumenal protein,is essential for photosystem I assembly in Arabidopsis. The Plant Cell, 2012, 24(12):4992-5006.
doi: 10.1105/tpc.112.106542
[18] Zhang L, Duan Z, Zhang J, et al. BIOGENESIS FACTOR REQUIRED FOR ATP SYNTHASE 3 facilitates assembly of the chloroplast ATP synthase complex. Plant Physiology, 2016, 171(2):1291-1306.
doi: 10.1104/pp.16.00248 pmid: 27208269
[19] 肖勇, 杨耀东, 夏薇, 等. 多倍体在植物进化中的意义. 广东农业科学, 2013, 40(16):127-130.
[20] Sun X, Peng L, Guo J, et al. Formation of DEG5 and DEG8 complexes and their involvement in the degradation of photodamaged photosystem II reaction center D1 protein in Arabidopsis. The Plant Cell, 2007, 19(4):1347-1361.
doi: 10.1105/tpc.106.049510
[21] 付爱根, 赵锋锋, 王娟, 等. 植物叶绿体细胞色素b6f复合体的研究进展. 山地农业生物学报, 2015, 34(6):1-8.
[22] 张琳, 彭连伟. 叶绿体ATP合酶生物发生的研究进展. 植物生理学报, 2019, 55(6):703-710.
[23] 陈熙, 崔香菊, 张炜. 低叶绿素b水稻突变体类囊体膜的比较蛋白质组学. 生物化学与生物物理进展, 2006, 33(7):653-659.
[24] López-Calcagno P E, Brown K L, Simkin A J, et al. Stimulating photosynthetic processes increases productivity and water-use efficiency in the field. Nature Plants, 2020, 6(8):1054-1063.
doi: 10.1038/s41477-020-0740-1 pmid: 32782406
[1] Pei Zhichao, Zhou Jihua, Xu Xiangdong, Lan Hongliang, Wang Junying, Lang Shuwen, Zhang Weiqiang. Effects of Drought Treatment on Photosynthesis Rate, Antioxidant Properties of Leaves and Yield of Different Maize Varieties [J]. Crops, 2021, 37(5): 95-100.
[2] Wang Gang, Yu Lihua, Zhao Huijie, Liu Yu, Geng Gui. The Effects of NaCl Stress on Growth and Photosynthesis of Sugarbeet at Different Growth Stages [J]. Crops, 2021, 37(4): 99-104.
[3] Yang Lei, Jin Yandi, Liu Houjun. Effects of Iron, Cadmium and Their Interaction on the Primary Reaction of Photosynthesis in Rice [J]. Crops, 2021, 37(4): 144-151.
[4] Zhao Baoping, Liu Jinghui, Ren Changzhong. Research Progress of Physiological Mechanism of Yield Formation in Oats [J]. Crops, 2021, 37(3): 1-7.
[5] Zhang Xuepeng, Li Teng, Wang Biao, Liu Qing, Liu Hanyu, Tao Zhiqiang, Sui Peng. Study on High Temperature Stress Threshold of Maize Leaves [J]. Crops, 2021, 37(2): 62-70.
[6] Wu Qiong, Ding Kaixin, Yu Minglong, Huang Wenting, Zuo Guanqiang, Feng Naijie, Zheng Dianfeng. Effects of New Plant Growth Regulator B2 on Photosynthetic Fluorescence Characteristics and Yield of Maize [J]. Crops, 2020, 36(5): 174-181.
[7] Li Ruijie,Tang Huihui,Wang Qingyan,Xu Yanli,Fang Mengying,Yan Peng,Dong Zhiqiang,Zhang Fenglu. Effects of 5- Aminolevulinic Acid and Ethylene Compounds on Photosynthetic Characteristics and Yield of Spring Maize in Northeast China [J]. Crops, 2020, 36(2): 125-133.
[8] Chen Ye,Nan Jing,Su Caijuan,Ma Yinshan,Wei Ning,Jing Yuxia. Effects of Astragalus membranaceus Root Water Extract on Seed Germination and Seedling Growth of Four Crops [J]. Crops, 2020, 36(2): 188-193.
[9] Jing Peipei,Ren Hongru,Yang Hongjian,Dai Qigen. Effects of Saline Stress on Leaf Photosynthesis Characteristics and Grain Yield of Two Rice Cultivars (Lines) [J]. Crops, 2020, 36(1): 67-75.
[10] Yudong Fang,Tianfu Han. Research Progress in Speed Breeding of Crops [J]. Crops, 2019, 35(2): 1-7.
[11] Xiaoming Yin,Chen Li. Differences in Leaf Photosynthesis and Assimilation of Nitrogen Between Two Rice Cultivars Differing in Nitrogen Use Efficiency [J]. Crops, 2019, 35(1): 90-96.
[12] Tang Liyuan,Li Xinghe,Zhang Sujun,Wang Haitao,Liu Cunjing,Zhang Xiangyun,Zhang Jianhong. QTL Mapping for Photosynthesis Related Traits in Upland Cotton [J]. Crops, 2018, 34(5): 85-90.
[13] Wang Jianan,Li Xiaoyan,Wei Shimei,Zhao Huijie,Zhao Mingqi,Wang Yuexia. Regulation of Exogenous 5-Aminolevulinic Acid on Photosynthesis and D1 Protein of Wheat Seedlings under Drought Stress [J]. Crops, 2018, 34(5): 121-126.
[14] Guangcai Zhao,Xuhong Chang,Demei Wang,Zhiqiang Tao,Yanjie Wang,Yushuang Yang,Yingjie Zhu. General Situation and Development of Wheat Production [J]. Crops, 2018, 34(4): 1-7.
[15] Baoquan Quan,Dongmei Bai,Yuexia Tian,Yunyun Xue. Effects of Different Leaf-Peg Ratio on Photosynthesis and Yield of Peanut [J]. Crops, 2018, 34(4): 102-105.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!