Crops ›› 2022, Vol. 38 ›› Issue (6): 132-138.doi: 10.16035/j.issn.1001-7283.2022.06.019

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Effects of Tobacco Planting Ecoregions, Varieties and Their Interactions on Polyphenol Content and Quality of Flue-Cured Tobacco

Chen Yan1(), Chen Qiang2, He Yi3, Yu Huiping3, Gao Junyi3, Zhao Erwei3, Lu Yingang1()   

  1. 1College of Agriculture, Guizhou University, Huaxi 550025, Guizhou, China
    2Tobacco College, Guizhou University, Huaxi 550025, Guizhou, China
    3Guizhou Province Tobacco Company Bijie City Company, Bijie 551700, Guizhou, China
  • Received:2022-01-27 Revised:2022-07-25 Online:2022-12-15 Published:2022-12-21
  • Contact: Lu Yingang E-mail:935190705@qq.com;agr.yglu@gzu.edu.cn

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Abstract:

To investigate the interactions between the flue-cured tobacco tpyes Yunyan 105 and Yunyan 116 and various ecological environments (such as light, rainfall, temperature, etc.) in the Bijie tobacco region, two-factor random block were designed. Yunyan 105 and Yunyan 116 were planted in Weining County and Dafang County, Bijie district, Guizhou Province, respectively, and the contents of polyphenols and conventional chemicals in tobacco leaves in the central part were determined and analyzed. The results showed that the contributions of tobacco-growing ecoregions, varieties and the contents of interactive polyphenols were different. The contribution rates of ecoregions to flue-cured tobacco neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, scopolamine, rutin and total phenols were 22.68 %, 7.68%, 9.67%, 94.40%, 9.48% and 3.14%, respectively; the contribution rates of varieties were 68.53%, 60.91%, 82.39%, 0.04%, 63.10% and 69.34%, respectively; the contribution rate of the interaction between ecological regions and varieties were 8.79%, 31.41%, 7.94%, 5.55%, 27.42% and 27.52%, respectively. Yunyan 105 had higher chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid and rutin contents in Dafang ecological tobacco area, while Yunyan 116 had higher scopolamine content in Weining. The polyphenol content of tobacco leaves were significantly positively correlated with the contents of phosphorus, potassium, reducing sugar and total sugar, and was significantly negatively correlated with the content of nicotine. In terms of polyphenols and chemical components of tobacco leaves, the newly introduced flue-cured tobacco varieties Yunyan 105 and Yunyan 116 in Bijie were more suitable for planting in Dafang area, and Yunyan 105 outperforms Yunyan 116.

Key words: Flue-cured tobacco, Variety, Ecoregion, Polyphenols, Chemical quality

Table 1

The rainfall, temperature of Dafang and Weining of Bijie city, Guizhou province"

月份
Month		 降雨量
Rainfall (mm)		 日均温度
Average daily temperature (℃)
大方
Dafang		 威宁
Weining		 大方
Dafang		 威宁
Weining
4月April 78.8 38.6 11.5 13.0
5月May 140.8 92.0 16.5 16.5
6月June 191.1 177.0 19.5 17.5
7月July 184.8 175.4 21.5 18.5
8月August 157.6 148.9 21.0 18.5

Fig.1

Chromatogram of polyphenol standard A: neochlorogenic acid, B: chlorogenic acid, C: cryptochlorogenic acid, D: scopolamine, E: rutin"

Table 2

The contents of polyphenols in different ecological intervals mg/g"

地区
Area		 新绿原酸
Neochlorogenic acid		 绿原酸
Chlorogenic acid		 隐绿原酸
Cryptochlorogenic acid		 莨菪亭
Scopolamine		 云香苷
Rutin		 总酚
Total phenols
大方Dafang 2.26±0.06a 13.37±0.21a 2.72±0.11a 0.25±0.01b 6.33±0.02a 24.93±0.37a
威宁Weining 1.99±0.02b 12.14±0.06b 2.47±0.11a 0.34±0.01a 6.76±0.20a 23.70±0.12b

Table 3

The content of polyphenols among different varieties mg/g"

品种
Variety		 新绿原酸
Neochlorogenic acid		 绿原酸
Chlorogenic acid		 隐绿原酸
Cryptochlorogenic acid		 莨菪亭
Scopolamine		 云香苷
Rutin		 总酚
Total phenols
云烟105 Yunyan 105 2.36±0.03a 14.49±0.26a 2.95±0.07a 0.30±0.02a 7.10±0.15a 27.21±0.01a
云烟116 Yunyan 116 1.89±0.07b 11.02±0.00b 2.24±0.07b 0.30±0.02a 5.98±0.32b 21.42±0.48b

Fig.2

Difference analysis of polyphenol contents in tobacco leaves among different treatments Different small letters indicate significant difference between different treatments (P < 0.05), the same below"

Table 4

Two-factor analysis of variance for the effects of interaction between ecoregions and varieties on polyphenols in flue-cured tobacco"

多酚物质
Polyphenol		 变异来源
Variation source		 平方和
Quadratic sum		 均方
Mean square		 F值
F-value		 P值
P-value		 贡献率
Contribution rate (%)
新绿原酸Neochlorogenic acid 生态区 0.22 0.22 39.59 0.0008 22.68
品种 0.67 0.67 119.63 0.0001 68.53
生态区×品种 0.09 0.09 15.36 0.0078 8.79
绿原酸Chlorogenic acid 生态区 4.57 4.57 26.48 0.0021 7.68
品种 36.21 36.21 209.93 0.0001 60.91
生态区×品种 18.67 18.67 108.25 0.0001 31.41
隐绿原酸Cryptochlorogenic acid 生态区 0.18 0.18 8.01 0.0300 9.67
品种 1.54 1.54 68.23 0.0002 82.39
生态区×品种 0.15 0.15 6.58 0.0427 7.94
莨菪亭Scopolamine 生态区 0.02 0.02 14.25 0.0092 94.40
品种 0.00 0.00 0.03 0.8739 0.04
生态区×品种 0.00 0.00 0.82 0.4003 5.55
云香苷Rutin 生态区 0.57 0.57 6.46 0.0440 9.48
品种 3.76 3.76 42.97 0.0006 63.10
生态区×品种 1.63 1.63 18.68 0.0050 27.42
总酚Total phenols 生态区 4.55 4.55 20.06 0.0042 3.14
品种 100.45 100.45 443.04 0.0001 69.34
生态区×品种 39.86 39.86 175.81 0.0001 27.52

Fig.3

Principal component analysis of polyphenols in different varieties of flue-cured tobacco in different ecological regions"

Table 5

Conventional chemical quality of flue-cured tobacco leaves in different treatments %"

处理Treatment 氮Nitrogen 磷Phosphorus 钾Potassium 还原糖Reducing sugar 总糖Total sugar 烟碱Nicotine
DF-105 1.62±0.06b 0.22±0.04a 2.49±0.19a 23.51±1.22a 29.56±0.78a 1.81±0.05c
DF-116 1.61±0.14b 0.19±0.02ab 1.93±0.05bc 21.63±1.00ab 27.87±0.80b 2.51±0.10a
WN-105 1.93±0.20a 0.16±0.01ab 2.02±0.10b 23.30±0.83ab 28.34±0.12b 2.17±0.17b
WN-116 1.99±0.10a 0.16±0.01b 1.78±0.06c 21.41±0.16b 28.10±0.25b 2.50±0.29a

Fig.4

Correlation analysis among various quality indexes of tobacco leaves in different treatments Neochlorogenic acid (NCA), Chlorogenic acid (CA), Cryptochlorogenic acid (CCA), Scopolamine (ChA), Rutin (R), Total phenols (TP), Nitrogen (N), Phosphorus (P), Potassium (K), Reducing sugar (RS), Total sugar (TS), Nicotine (Nt).“*”: P < 0.05;“**”: P < 0.01;“***”: P < 0.001"

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