Crops ›› 2023, Vol. 39 ›› Issue (2): 193-200.doi: 10.16035/j.issn.1001-7283.2023.02.028

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Effects of Shading and Light-Transmitting Treatment on Lipid Peroxidation Characteristics of Cigar Leaves during Air-Curing and Chemical Composition after Air-Curing

Chen Yongwei1(), Jin Mingke1, Wu Yongbing1, He Zhengchuan2, Yang Weili2(), Zhao Mingqin1()   

  1. 1College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, Henan China
    2Sichuan Dazhou Municipal Tobacco Company, Dazhou 635000, Sichuan, China
  • Received:2022-01-27 Revised:2022-10-15 Online:2023-04-15 Published:2023-04-11

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Abstract:

To investigate the impact of various shading treatments on the air-curing effect of cigars, the middle leaves of Chuanxue No.1 were used as the material and the five light penetration of shading treatments with 100%, 85%, 70%, 55%, and 40% were set to investigate their effects on the features of membrane lipid peroxidation during the air-curing process of cigar leaves. The results showed that, the moisture content of shadow treatments with 55%-70% light penetration was significantly higher than that of other treatments. Also the water loss rate was moderate under the 70% shading and light-transmitting treatment, which aided in improving color change and material transformation coordination. The MDA content of the 55%-70% shading and light-transmitting treatment was much lower than that of the other treatments. Furthermore, the activities of POD, CAT, SOD, and PPO were continuous high activity, which contributed to reduce membrane lipid peroxidation. The concentrations of chlorogenic acid, rutin, and scopoletin were maintained an appropriate level at all stages during the shadow treatments with 70% light penetration, which was beneficial to the antioxidation and quality formation of cigar leaves. The chemical composition of cigar leaves with 70% shading and light-transmitting treatment exhibited the best coordination after the air-curing. To summarize, the cigar leaves with 70% shading and light-transmitting treatment demonstrated good coordination of water loss rate, great enzyme activity and significant antioxidant capacity during the air-curing process, ensuring the coordination of enzymatic browning reaction process and substance transformation in tobacco leaves and improving the air-curing effects of tobacco leaves.

Key words: Shading, Cigars, Air-curing, Membrane peroxidation, Moisture content, Enzyme activity, Polyphenols

Table 1

Effects of shade and light transmission on moisture content of cigar leaves during air-curing %"

处理
Treatment		 晾制时间Airing time
0d 5d 10d 15d 20d 25d
CK 86.86±1.12c 78.06±2.09b 55.71±1.27c 29.48±1.21b 24.51±2.01b 15.52±0.82b
T1 87.63±1.45bc 79.28±1.89b 57.16±2.37c 31.76±2.01ab 25.45±1.10ab 16.52±0.85b
T2 89.57±1.32ab 83.34±1.43ab 61.98±1.62b 33.97±2.41a 27.41±1.68ab 18.64±1.13a
T3 89.93±1.21ab 84.55±0.81ab 62.71±1.81ab 34.74±2.18a 28.13±1.26a 20.19±1.29a
T4 90.59±1.85a 86.44±1.23a 65.42±1.69a 31.33±2.25ab 26.07±1.98ab 16.16±1.02b

Fig.1

Effects of shading and light-transmitting treatment on MDA content during the drying process of cigars Different letters indicate significant difference at the P < 0.05 level, the same below"

Fig.2

Effects of shading and light transmission on the activities of POD, CAT and SOD during the curing process of cigar leaves"

Fig.3

Effects of shading and light-transmitting treatment on the activity of PPO during the curing process of cigar tobacco leaves"

Fig.4

Effects of shading and light transmission on the content of polyphenols during the drying process of cigar leaves"

Table 2

Correlation analysis between moisture content and membrane lipid peroxidation products in shading and light-transmitting cigar leaves during air-curing"

指标
Index		 处理
Treatment		 MDA POD CAT SOD PPO 绿原酸
Chlorogenic acid		 芸香苷
Rutin		 莨菪亭
Scopoletin
含水率
Moisture content		 CK -0.974** 0.670 0.892* 0.995** 0.542 0.959** 0.938** 0.871*
T1 -0.985** 0.666 0.905* 0.997** 0.526 0.964** 0.952** 0.878*
T2 -0.988** 0.674 0.920** 0.997** 0.553 0.960** 0.936** 0.881*
T3 -0.984** 0.681 0.912* 0.997** 0.552 0.955** 0.971** 0.882*
T4 -0.985** 0.735 0.927** 0.997** 0.655 0.923** 0.951** 0.939**

Table 3

Correlation analysis of membrane lipid peroxidation characteristics during the drying process of cigar leaves treated with shade and light transmission"

指标
Index		 处理
Treatment		 POD CAT SOD PPO 绿原酸
Chlorogenic acid		 芸香苷
Rutin		 莨菪亭
Scopoletin
MDA CK -0.674 -0.871* -0.977** -0.630 -0.928** -0.940** -0.916*
T1 -0.674 -0.886* -0.982** -0.595 -0.962** -0.961** -0.920**
T2 -0.669 -0.901* -0.992** -0.608 -0.957** -0.956** -0.923**
T3 -0.697 -0.906* -0.991** -0.633 -0.941** -0.983** -0.929**
T4 -0.750 -0.921** -0.981** -0.722 -0.894* -0.984** -0.971**

Table 4

The effects of different shading treatments on the physical properties of cigar tobacco leaves after air-curing"

处理
Treatment		 总糖
Total sugar
(%)		 还原糖
Reducing sugar
(%)		 总氮
Total nitrogen
(%)		 烟碱
Nicotine
(%)		 氯
Chlorine
(%)		 钾
Potassium
(%)		 两糖比
Two sugar
ratio		 钾氯比
Potassium
chlorine ratio		 氮碱比
Nitrogen
nicotine ratio
CK 1.23±0.03c 0.92±0.02d 3.91±0.04a 1.82±0.05c 0.20±0.03b 2.91±0.13e 0.74±0.02bc 14.02±0.80b 2.14±0.02a
T1 1.38±0.01b 1.08±0.03b 3.90±0.02a 2.17±0.03a 0.24±0.03ab 3.35±0.09d 0.78±0.01ab 13.94±1.19b 1.79±0.02b
T2 1.65±0.03a 1.33±0.03a 3.05±0.07c 2.08±0.05b 0.27±0.02a 4.83±0.08a 0.81±0.03a 17.56±0.62a 1.46±0.07c
T3 1.42±0.03b 0.99±0.02c 3.87±0.04a 2.12±0.03ab 0.28±0.04a 4.59±0.06b 0.69±0.02c 16.28±0.32a 1.82±0.03b
T4 0.91±0.02d 0.68±0.04e 3.38±0.06b 2.18±0.03a 0.26±0.03a 3.85±0.12c 0.74±0.05bc 14.52±0.83b 1.55±0.02d
[1] 李爱军, 秦艳青, 代惠娟, 等. 国产雪茄烟叶科学发展刍议. 中国烟草学报, 2012, 18(1):112-114.
[2] 金敖熙. 雪茄烟生产技术. 北京: 轻工业出版社,1982.
[3] 宋凤鸣, 葛秀春, 郑重, 等. 活性氧及膜脂过氧化与棉花对枯萎病抗性的关系. 植物病理学报, 2001(2):110-116.
[4] 黄山, 杨虹琦, 张发明, 等. 烘烤温湿度变化对不同烤烟品种烟叶膜脂过氧化作用的影响. 湖南农业大学学报(自然科学版), 2009, 35(5):485-492.
[5] 陈秋芳, 郭月清, 宫长荣, 等. 白肋烟调制过程中叶片膜脂过氧化特性的研究. 河南农业大学学报, 1997(4):24-27.
[6] 过伟民, 张骏, 刘阳, 等. 烤烟质体色素及多酚与外观质量关系研究. 中国烟草学报, 2009, 15(2):33-40.
[7] 赵松超, 田培, 刘博远, 等. 采收成熟度对雪茄烟叶晾制过程酶促棕色化反应及品质的影响. 中国农业科技导报, 2020, 22(5):51-59.
doi: 10.13304/j.nykjdb.2019.0100
[8] 孙延国, 刘好宝, 高华军, 等. 移栽期对海南雪茄外包皮烟叶生长发育及产量品质的影响. 中国烟草科学, 2019, 40(3):91- 98.
[9] 张锐新, 任天宝, 赵喆, 等. 移栽期对五指山雪茄烟品质及主要经济性状的影响. 作物杂志, 2018(2):148-153.
[10] 卢瑞琳, 刘路路, 胡希, 等. 不同采收时间对雪茄烟叶产质量的影响. 河南农业科学, 2021, 50(10):51-59.
[11] 刘慧, 赵松超, 武志勇, 等. 不同采收方式对雪茄烟上部叶晾制效果的影响. 中国烟草学报, 2021, 27(3):65-72.
[12] 张嘉雯, 卢绍浩, 赵铭钦, 等. 植烟密度对雪茄烟叶碳氮代谢及品质的影响. 中国烟草科学, 2020, 41(4):95-100.
[13] 国家烟草专卖局. YC/T 31-1996 烟草及烟草制品试样的制备和水分的测定―烘箱法. 北京: 中国标准出版社,1996.
[14] 国家烟草专卖局. 烟草及烟草制品多酚类化合物绿原酸、莨菪亭和芸香苷的测定. 北京: 中国标准出版社, 2006.
[15] 汤章城. 现代植物生理学实验指南. 北京: 科学出版社,1999.
[16] 周冀衡. 烟草生理与生物化学. 合肥: 中国科学技术大学出版社,1996.
[17] 赵松超, 李一凡, 刘博远, 等. 晾制密度对雪茄烟叶膜脂过氧化作用及品质的影响. 作物学报, 2019, 45(7):1090-1098.
doi: 10.3724/SP.J.1006.2019.84134
[18] 文志强. 遮荫对雪茄外包皮烟生长发育和生理特性的影响. 郑州:河南农业大学, 2007.
[19] 吴晓颖. 光强对雪茄烟叶片生长及生理生化特性的影响. 北京: 中国农业科学院, 2021.
[20] 顾会战. 调制温湿度条件对雪茄外包皮烟叶生理变化与理化特性的影响. 郑州:河南农业大学, 2006. 201 819c9509-8f43-44b8-a51e-f53f3f4a4681
[21] 刘柿良, 马明东, 潘远智, 等. 不同光强对两种桤木幼苗光合特性和抗氧化系统的影响. 植物生态学报, 2012, 36(10):1062-1074.
[22] 杜秀敏, 殷文璇, 赵彦修, 等. 植物中活性氧的产生及清除机制. 生物工程学报, 2001, 17(2):121-125.
[23] 韩锦峰. 烟草栽培生理. 北京: 中国农业出版社, 2003.
[24] 李吴阳, 夏继桥, 杨连玉, 等. 植物多酚的抗氧化能力及其在动物生产中的应用. 动物营养学报, 2013, 25(11):2529-2534.
[25] 卢绍浩, 张嘉雯, 赵喆, 等. 不同部位雪茄烟叶晾制过程中多酚、色素及相关酶活性的变化. 中国烟草科学, 2019, 40(3):84-90,98.
[26] 范宁波, 蔡斌, 张瑞娜, 等. 雪茄烟叶晾制过程色素降解和膜脂过氧化对颜色变化的影响. 中国烟草学报, 2021, 27(2):102-108.
[27] 范宁波, 张瑞娜, 路晓崇, 等. 雪茄烟叶晾制过程中颜色与水分含量和膜脂过氧化的关系. 中国烟草科学, 2020, 41(6):96-102.
[28] 朱金峰, 王涵, 王国峰, 等. 植物生长物质对不同烘烤工艺下烟叶多酚含量及其酶活性的影响. 河南农业科学, 2013, 42 (3):36-41.
[29] 宫长荣. 烟草调制学. 北京: 中国农业出版社, 2003.
[30] 李秀妮, 乔保民, 吴创, 等. 晾晒烟调制过程中酶促棕色化反应及调控方法. 天津农业科学, 2018, 24(2):76-79.
[31] 柴家荣, 李天飞, 杨宏光, 等. 白肋烟TN86晾制期PPO、POD活性及总酚、类黄酮含量动态研究. 中国烟草科学, 2003, 24(1):31-33.
[32] 寇明钰, 李宁, 吴艳, 等. 棕色化反应调控技术在烟草中的应用现状及展望. 湖北农业科学, 2009, 48(8):2013-2016.
[33] 宫长荣, 王爱华, 王松峰, 等. 烟叶烘烤过程中多酚类物质的变化及与化学成分的相关分析. 中国农业科学, 2005, 38(11):2316-2320.
[34] 中国科学院北京植物研究所鸭梨黑心病研究小组. 鸭梨黑心病的研究:Ⅱ. 酚类物质的酶促褐变. 植物学报, 1974, 16(3):235-242.
[35] 刘国顺. 烟草栽培学. 北京: 中国农业出版社, 2003.
[36] 李爱军, 范静苑, 秦艳青, 等. 海南与印尼茄衣烟叶质量差异分析. 中国烟草学报, 2013, 19(4):60-63.
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