Crops ›› 2023, Vol. 39 ›› Issue (2): 186-192.doi: 10.16035/j.issn.1001-7283.2023.02.027

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Research on Contribution of Glutamic Acid to Nitrogen Nutrition and Physiological Characteristics of Flue-Cured Tobacco

Zhang Yifei1(), Zu Qingxue1, Nie Zhongyang1, Lin Song1, Rao Chen1, Cheng Zhijun2   

  1. 1Kaiyang Branch of Guizhou Tobacco Company, Kaiyang 550300, Guizhou, China
    2China Tobacco Hunan Industrial Co., Ltd., Changsha 410000, Hunan, China
  • Received:2021-11-30 Revised:2022-01-10 Online:2023-04-15 Published:2023-04-11

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Abstract:

In order to explore the effects of amino acid nitrogen on the growth and development of flue-cured tobacco and the contribution of nitrogen nutrition, using Nanjiang No.3 as the material, L-glutamic acid as the organic nitrogen source, 15NH415NO3 as the inorganic nitrogen source, five treatments with different nitrogen source ratios were set up: no nitrogen application (CK), 100% inorganic nitrogen (T1), 25% glutamate nitrogen + 75% inorganic nitrogen (T2), 50% glutamate nitrogen + 50% inorganic nitrogen (T3), 75% glutamate nitrogen + 25% inorganic nitrogen (T4), 100% glutamate nitrogen (T5), and the tobacco plant biomass, nitrogen content and related enzyme activities were measured. The results showed that, the application of glutamic acid had a certain inhibitory effects on the biomass and nitrogen content of tobacco plants in the early growth period, and the most obvious inhibitory effects was at the rosette stage. In the later growth period, 25%-50% of amino acids could promote the growth of tobacco plants. With the increased of the proportion of glutamate, the proportion of total nitrogen content in the upper and middle tobacco leaves increased with different degrees, while the proportion of total nitrogen in the lower tobacco leaves gradually decreased from 16.77% to 8.03%, the application of glutamate was conducive to the accumulation of nitrogen content in the middle and upper leaves of the tobacco plant. The activities of GPT and GDH of plant roots, stems and leaves showed an increasing trend with the increase of the proportion of glutamate. It showed that, flue-cured tobacco could directly absorb and utilize glutamate molecules. The combination of low-concentration glutamate and inorganic nitrogen could promote the growth of tobacco plants and regulate the distribution of nitrogen in tobacco plants. However, high concentrations of glutamate inhibited the growth of tobacco plants.

Key words: Flue-cured tobacco, Glutamic acid, 15N, Growth, Nitrogen contribution

Fig.1

Effects of different treatments on the biomass of roots, stems and leaves of tobacco plants at different growth stages Different lowercase letters indicate significant differences among treatments (P < 0.05), the same below"

Fig.2

Nitrogen content in roots, stems and leaves of tobacco plants at different growth stages of each treatment"

Table 1

"

处理Treatment 上部叶Upper leaf 中部叶Middle leaf 下部叶Lower leaf 茎Stem 根Root 15N总量Total 15N
CK － － － － － －
T1 0.48±0.04b 0.64±0.03a 0.32±0.02a 0.76±0.06a 0.33±0.03a 2.53±0.15a
T2 0.45±0.04b 0.55±0.06b 0.34±0.02a 0.55±0.03b 0.23±0.02b 2.12±0.16c
T3 0.57±0.05a 0.70±0.04a 0.22±0.02b 0.49±0.07b 0.25±0.02b 2.23±0.12b
T4 0.17±0.03c 0.22±0.02c 0.09±0.00c 0.19±0.03c 0.11±0.02c 0.78±0.08d
T5 － － － － － －

Fig.3

Proportion of 15N in each part of tobacco plants with different treatments"

Fig.4

Effects of different treatments on nitrogen absorption in various parts of tobacco plants"

Fig.5

Effects of different treatments on the activity of GPT in leaf, stem and root of tobacco plants"

Fig.6

Effects of different treatments on the activity of GDH in leaf, stem and root of tobacco plants"

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