Crops ›› 2023, Vol. 39 ›› Issue (3): 188-194.doi: 10.16035/j.issn.1001-7283.2023.03.026

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Effects of Salicylic Acid Application on the Growth and Physiological Characteristics of Cotton Seedlings under Salt Stress

Xu Xuewen1,2(), Wang Xingpeng1,2,3, Wang Hongbo1,2, Li Guohui1,2, Tang Maosong1,2, Cao Zhenxi1,2()   

  1. 1College of Water Resources and Construction Engineering, Tarim University, Alar 843300, Xinjiang, China
    2Key Laboratory of Modern Agricultural Engineering, Tarim University, Alar 843300, Xinjiang, China
    3Key Laboratory of Water-Saving Agriculture in Northwest Oasis, Ministry of Agriculture and Rural Affairs, Shihezi 832000, Xinjiang, China
  • Received:2023-02-16 Revised:2023-03-04 Online:2023-06-15 Published:2023-06-16

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Abstract:

To investigate the regulatory effects of salicylic acid (SA) on the growth and physiology of cotton seedlings under salt stress, two salt concentrations of 3 (low salt, T1) and 6g/L (high salt, T2) and four SA concentrations of 0.00 (S0), 0.01 (low concentration, S1), 0.05 (medium concentration, S2) and 0.10mmol/L (high concentration, S3) were set, and no SA, no NaCl was as the control (CK), and the effects of SA on root dry matter mass, root morphology, antioxidant enzymes and osmoregulatory substances of cotton seedlings under salt stress were investigated based on a comprehensive evaluation method of principal components. The effects of SA on root dry matter, root morphology, antioxidant enzymes and osmoregulatory substances of cotton seedlings under salt stress were investigated based on a comprehensive evaluation method. Principal component analysis showed that cotton seedling root surface area, underground dry matter, root volume and catalase (CAT) activity were the most responsive to salt stress and SA regulation, and showed a trend of increasing and then decreasing with increasing SA concentration at the same salt concentration, with T1S2 treatment increasing 66%, 75%, 119% and 26% compared to T1S0, respectively; T2S2 treatment increasing 47%, 61%, 49% and 12% compared to T2S0, respectively. With increasing salt concentration, root surface area, underground dry matter, and root volume showed a significant decrease, compared with T1S0, T2S0 treatment decreasing 33%, 25% and 22%, respectively, while CAT activity showed a significant increase with the increasing of salt concentration, T2S0 treatment increasing 32% compared with T1S0 treatment. The comprehensive evaluation showed that the medium concentration of SA (0.05mmol/L) alleviated the damage of low salt stress on cotton seedlings, and the alleviating effect of SA decreased with the increasing of salt concentration. The results could provide a more suitable exogenous application strategy for salt-tolerant and stress-resistant planting of cotton.

Key words: Salt stress, Salicylic acid, Root morphological indexes, Antioxidant enzymes, Osmotic regulating substance, Comprehensive evaluation

Fig.1

Cotton seedling growth device"

Table 1

Effects of SA on root index, bioaccumulation and root-shoot ratio of cotton seedlings under salt stress"

处理
Treatment		 根总长度
Total length
of root (cm)		 根表面积
Surface area
of root (cm2)		 根体积
Volume of
root (cm3)		 地下部干物质
Underground
dry matter (mg)		 地上部干物质
Above-ground
dry matter (mg)		 根冠比
Root-shoot
ratio (%)
CK 43.43±3.73a 30.47±1.82a 2.00±0.18a 34.37±0.87a 163.40±5.82a 21.04±0.32a
T1S1 31.24±1.96c 17.55±0.99c 1.04±0.14b 18.50±1.15cd 112.67±8.39de 16.52±2.04bc
T1S2 41.88±0.89a 28.69±0.74a 1.88±0.09a 33.87±1.50a 163.10±8.43a 20.78±0.85a
T1S3 36.28±3.78b 20.69±2.30b 1.02±0.12b 22.00±3.03b 132.67±9.36b 16.55±1.47bc
T1S0 30.13±2.02c 17.28±1.08c 0.86±0.06bc 19.40±0.98c 130.10±7.18bc 14.91±0.08c
T2S1 20.06±1.58d 13.78±2.29d 0.78±0.08c 16.90±1.14cde 103.30±1.21e 16.36±1.12bc
T2S2 28.59±3.33c 17.06±2.55c 1.00±0.12b 23.33±0.59b 133.03±3.18b 17.54±0.18b
T2S3 19.86±2.27d 10.18±1.65e 0.50±0.05d 16.27±1.11de 125.50±5.01bc 12.96±0.79d
T2S0 18.02±1.23d 11.58±0.62de 0.67±0.04cd 14.53±1.12e 119.73±5.71cd 12.14±0.82d
T ** ** ** ** ** **
S ** ** ** ** ** **
T×S ns ** ** ** * *

Fig.2

Effects of SA on primary root length of cotton seedlings under different salt concentrations"

Fig.3

Antioxidant enzyme activities, MDA content and osmoregulatory substance content of cotton seedling roots under SA treatment under salt stress The different lowercase letters indicate significant difference at P < 0.05 level"

Table 2

Principal component eigenvalues, contribution rates and cumulative contribution rates"

主成分
Principal
component		 X1 X2 X3 X4 X5 X6 X7 X8 X9 X10 X11 X12 X13 特征值
Eigenvalue		 贡献率
Contribution
rate (%)		 累积贡献率
Cumulative
contribution rate (%)
PC1 0.97 0.98 0.81 0.93 0.93 0.98 0.98 0.36 -0.03 0.24 -0.84 0.03 0.02 7.11 54.68 54.68
PC2 -0.14 0.11 0.05 0.13 -0.29 -0.15 0.02 0.90 0.98 0.84 0.31 0.97 0.97 4.60 35.42 90.10

Table 3

Principal component score coefficients"

主成分Principal component X1 X2 X3 X4 X5 X6 X7 X8 X9 X10 X11 X12 X13
PC1 0.33 0.34 0.28 0.32 0.32 0.34 0.34 0.12 -0.01 0.08 -0.29 0.01 0.01
PC2 -0.08 0.06 0.02 0.07 -0.15 -0.08 0.01 0.48 0.52 0.45 0.16 0.51 0.52

Table 4

Composite score of principal component analysis"

项目Item T1S1 T1S2 T1S3 CK1 T2S1 T2S2 T2S3 CK2
Y1 -0.48 3.59 0.27 -0.76 -1.68 0.30 -2.42 -2.41
Y2 -0.58 0.98 -1.45 -2.24 1.14 4.40 0.75 1.49
Y -0.47 2.32 -0.36 -1.20 -0.52 1.71 -1.07 -0.81
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