Crops ›› 2023, Vol. 39 ›› Issue (3): 195-199.doi: 10.16035/j.issn.1001-7283.2023.03.027

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Analysis on the Relationship between Contents of Free Amino Acids and the Quality of Flue-Cured Tobacco in Eight Flavor Ecological Regions of China

Jia Guotao1(), Wang Xiaoyu2, Sun Yiming1, Nie Cong2, He Jingyu1, Feng Yingjie1, Ma Shengtao1, Cui Ting1, Cheng Dongxu1, Yao Qian1, Li Yue1, Zhang Ziying1, Wang Baolin1(), Liu Huimin2()   

  1. 1China Tobacco Henan Industry Co., Ltd., Zhengzhou 450000, Henan, China
    2Zhengzhou Tobacco Research of CNTC, Zhengzhou 450001, Henan, China
  • Received:2022-10-08 Revised:2022-03-28 Online:2023-06-15 Published:2023-06-16

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Abstract:

In order to analyze the content difference and correlation of free amino acids in flue-cured tobacco in different ecological regions, as well as the relationship with chemical quality indicators, 249 tobacco leaf samples from eight different flavor style flue-cured tobacco ecological regions were taken as the research objects. The results showed the total amount of free amino acids in flue-cured tobacco was upper leaves>middle leaves> lower leaves, with the highest content of Pro, followed by Asn, and the content of Met was the lowest. The contents of Asn, Glu, Gln, Gly, Ala, Lys, His and Trp in flue-cured tobacco were upper leaves>lower leaves> middle leaves. The free amino acids in flue-cured tobacco were mainly positively correlated. Based on the heat map analysis, the total amount of free amino acids in flue-cured tobacco in different ecological regions was IV> VII>I>II>III>VIII>V>VI. The Ser contents in the national high-quality tobacco production areas I, II and III were high, and the total amount of amino acids were at a medium level. The total sugar, reducing sugar, potassium and sugar base ratio in different ecological regions were negatively correlated with most free amino acids.

Key words: Flue-cured tobacco, Free amino acids, Ecological region, Quality, Correlation

Table 1

Information of tobacco sample"

编号
Number		 生态区域
Ecological region		 香型
Fragrance		 样品数量
Number of samples
Ⅰ 西南高原 清甜香型 42
Ⅱ 黔桂山地 蜜甜香型 24
Ⅲ 武陵秦巴 醇甜香型 41
Ⅳ 黄淮平原 焦甜焦香型 43
Ⅴ 南岭丘陵 焦甜醇甜香型 39
Ⅵ 武夷丘陵 清甜蜜甜香型 33
Ⅶ 沂蒙丘陵 蜜甜焦香型 12
Ⅷ 东北平原 木香蜜甜香型 15

Table 2

Comparison of free amino acid contents in different parts of flue-cured tobacco μg/g"

指标
Index		 烟叶等级Tobacco grade
B2F C3F X2F
天冬氨酸Asp 224.04±9.40b 206.68±9.92b 253.27±11.89a
苏氨酸Thr 66.70±3.19a 47.57±1.92b 60.19±2.44a
丝氨酸Ser 299.48±7.54a 292.64±6.58a 308.61±6.89a
天冬酰胺Asn 1383.41±97.21a 748.34±62.73b 905.67±80.81b
谷氨酸Glu 255.75±13.64a 184.26±10.24b 255.75±13.64b
谷氨酰胺Gln 890.51±60.91a 511.14±36.84b 624.81±46.89b
甘氨酸Gly 34.91±1.12a 23.64±0.67b 25.66±0.76b
丙氨酸Ala 595.13±13.23a 495.64±9.78b 518.88±11.08b
缬氨酸Val 412.00±7.03b 421.11±6.95b 459.47±7.95a
胱氨酸Cys 74.42±2.66a 63.33±2.27b 56.94±1.26c
蛋氨酸Met 4.38±0.18a 4.69±0.23a 4.29±0.21a
异亮氨酸Ile 7.79±0.26a 6.68±0.22b 7.99±0.29a
亮氨酸Leu 18.42±0.75a 13.44±0.56c 16.47±0.65b
酪氨酸Tyr 56.60±2.15a 49.52±1.86b 54.56±2.12ab
苯丙氨酸Phe 178.55±11.55a 135.92±9.42b 176.54±12.22a
氨基丁酸
4-Gaba		 210.92±9.76a 150.48±6.22c 184.17±7.45b
赖氨酸Lys 39.85±2.60a 23.35±1.56b 24.29±1.38b
组氨酸His 158.05±9.39a 96.41±5.83b 109.34±6.83b
色氨酸Trp 184.03±10.68a 136.05±7.85b 157.50±9.77b
精氨酸Arg 41.19±2.16a 27.95±1.32b 25.63±1.15b
脯氨酸Pro 10 421.86±326.93a 8011.98±221.12b 7050.80±199.89c
总量Total 15 555.79±514.76a 11 650.82±334.43b 11 280.83±347.99b

Fig.1

Correlation analysis of free amino acid contents in flue-cured tobacco “*”,“**”and“***”indicate significant correlation at the levels of 0.05, 0.01 and 0.001, respectively"

Fig.2

Heatmap of free amino acid contents in flue-cured tobacco in different ecological regions"

Fig.3

Correlation analysis between free amino acid contents and conventional chemical quality indexes of flue-cured tobacco"

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