Crops ›› 2023, Vol. 39 ›› Issue (3): 20-26.doi: 10.16035/j.issn.1001-7283.2023.03.003

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Differences Research in Salt Tolerance of New Rice Lines at Seedling Stage in Liaoning

Luan Jinhua1,2(), Song Xinyang1, Wang Lei1, Sun Lili1, Cheng Yanshuang1, Dong Hao1, Zhang Jia3, Cheng Xiaoyi1(), Xu Hai1()   

  1. 1Institute of Rice Research, Shenyang Agricultural University/Key Laboratory of Northern Japonica Genetics and Breeding of Liaoning Province/Key Laboratory of Northeast Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs/Key Laboratory of Northern Japonica Super Rice Breeding, Ministry of Education, Shenyang 110866, Liaoning, China
    2Qingdao Haidel Biotechnology Co., Ltd., Qingdao 266000, Shandong, China
    3Modern Agricultural Production Base Construction Engineering Center in Liaoning, Shenyang 110866, Liaoning, China
  • Received:2022-05-10 Revised:2022-06-30 Online:2023-06-15 Published:2023-06-16

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Abstract:

Screening and cultivating rice varieties with strong salt tolerance is of great significance for making full use of land resources and ensuring national food security. The total of 118 new rice lines newly bred in Liaoning province in recent years were used as experimental materials. Through different concentrations of NaCl solution treatment at seedling stage, the difference of seedling quality between different lines were compared, and the new rice lines with strong salt tolerance were screened. The results showed that germination rate was the most important characteristics in the evaluation of salt tolerance of rice at seedling stage, and the variation coefficient of root weight under salt stress was 83.29%, which also accounted for a large proportion in principal component analysis. Therefore, it was considered that the response of the two traits to salt stress were stable, and the changes of the two traits could be used as reference indexes for salt tolerance evaluation. Combined with the evaluation results of seedling quality and principal component analysis, five new rice lines with strong salt tolerance were selected, which were Yanjing 352, Huajing 1520, Yanjing 172, Shenxingdao 28 and Huajing 1505.

Key words: Rice, Seedling stage, Salt stress, Salt tolerance, Seedling quality

Table 1

Names and sources of new lines"

序号Number 品系名称Line name 育种单位Breeding unit
1~13
 SN1247、SN663、SN1101、YJ1232、YJR32、YJP2、YJ1113、沈稻6号、沈农稻551、沈农稻513、沈农稻517、沈农稻97、沈农稻142 沈阳农业大学农学院
14~28
 SNR1、SNR2、SNR3、SNR4、SNR5、SNR6、SNR7、SNR8、SNR9、SNR10、SNR11、SNR12、SNR13、SNR14、SNR15 沈阳农业大学水稻研究所
29~35 DS901、DS861、D11、D35、DS798、DS821、D844 辽宁东亚种业有限公司
36 辽粳169 辽宁省农业科学院创新中心
37~58

 盐粳390、盐粳403、盐粳756、盐粳757、盐粳758、盐粳759、花粳1520、花粳1504、花粳1505、花粳1508、花粳14Q6、盐粳39、盐粳46、盐粳227、盐粳352、盐粳171、盐粳172、盐粳173、花粳1611、花粳1607、花粳1609、花粳1624 辽宁省盐碱地利用研究所

59~60 TL628、TL13 天津天隆科技有限公司
61~76
 LDC38、LDZ16-1、LDZ16-2、LDC8、LDC16、G248、G278、G169、G297、G233、G242、G362、G305、辽粳9号、辽99优27、辽99优101 辽宁省水稻研究所
77~79 东研稻20、东研稻23、港优7号 东港市示范繁殖农场
80~82 丹1634、丹粳优3号、丹粳优5号 丹东农业科学院
83~89 沈星稻11、沈星稻240、沈星稻79、沈星稻27、沈星稻28、沈星稻4、沈星稻5 沈阳市北星水稻研究所
90~93 营育2、阳光48、阳光60、营育44 大石桥市阳光种业有限公司
94~95 开513、开734 个人
96~102 铁163、铁164、铁1702、铁1712、铁165、铁166、铁1708 铁岭市农业科学院
103~106 佳选稻05、佳选稻06、佳选稻137、佳选稻139 沈阳沃土同创科技有限公司
107~110 沈稻61、沈稻97、沈稻68、沈裕粳1号 沈阳裕赓种业有限公司
111 乾稻3号 大洼县秦那种业有限公司
112~113 盐星13-8、盐星898 个人
114~118 桥选稻161、桥选稻164、桥选稻171、桥选稻172、桥选稻173 大石桥市种子有限公司

Table 2

Classification standard of relative salt damage"

等级
Grade		 相对盐害率
Relative salt damage rate (%)		 耐盐性
Salt resistance
1 0.0~20.0 极强
3 20.1~40.0 强
5 40.1~60.0 中
7 60.1~80.0 弱
9 80.1~100.0 极弱

Table 3

Variance analysis between salt concentration and germination rate"

差异源Source of variation SS df MS F P-value F crit
组间Inter-group 18.41 3 6.140 186.88 1.93E-79 2.62
组内Intra-group 15.23 464 0.033
总计Total 33.64 467

Fig.1

Effects of salt stress on germination percentage of rice Different lowercase letters indicate significant difference between treatments, the same below"

Table 4

Correlation coefficients of seedling quality under salt stress"

指标
Index		 盐浓度
Salt concentration		 发芽率
Germination percentage		 苗高
Seedling height		 根长
Root length		 根数
Number of roots		 苗重
Seedling weight
发芽率Germination percentage -0.451**
苗高Seedling height -0.761** 0.411**
根长Root length -0.259 0.191* 0.325*
根数Number of roots -0.271 0.339* 0.523* 0.148*
苗重Seedling weight -0.599** 0.133* -0.178** 0.004 -0.147*
根重Root weight -0.567** 0.600** 0.464** 0.140* 0.433** -0.120

Fig.2

Effects of salt stress on rice seedling quality"

Table 5

Evaluation of salt tolerance and number of materials"

等级
Grade		 相对盐害率
Relative salt damage rate (%)		 耐盐性
Salt tolerance		 材料数目
Material number
1 0.0~20.0 极强 10
3 20.1~40.0 强 17
5 40.1~60.0 中 32
7 60.1~80.0 弱 28
9 80.1~100.0 极弱 31

Table 6

Effects of 0.5% salt concentration on rice seedling growth"

指标
Index		 对照Control 盐胁迫Salt stress
最大值
Maximum		 最小值
Minimum		 平均值
Average		 标准差
Standard
deviation		 变异系数
Coefficient of
variation (%)		 最大值
Maximum		 最小值
Minimum		 平均值
Average		 标准差
Standard
deviation		 变异系数
Coefficient of
variation (%)
发芽率Germination percentage (%) 100.00 37.50 84.40 0.13 15.49 100.00 12.50 48.49 0.18 37.46
苗高Seedling height (cm) 16.50 3.00 9.88 2.55 25.77 9.20 3.20 6.20 1.26 20.31
根长Root length (cm) 8.80 2.20 5.32 1.42 26.68 8.80 1.70 4.43 1.62 36.62
根数Roots number 8.00 4.00 5.42 0.82 15.14 5.00 2.00 4.62 0.72 15.51
苗重Seedling weight (g) 0.15 0.14 0.15 0.01 8.73 0.06 0.01 0.04 0.03 87.12
根重Root weight (g) 0.09 0.07 0.09 0.01 14.14 0.05 0.01 0.04 0.03 83.29

Table 7

Eigen value and contribution rate of components"

成分
Component		 成分特征值Component eigen value 平方和载入Sum of squares
总计
Total		 贡献率
Contribution rate (%)		 累计贡献率
Cumulative contribution rate (%)		 总计
Total		 贡献率
Contribution rate (%)		 累计贡献率
Cumulative contribution rate (%)
1 2.863 47.717 47.717 2.863 47.717 47.717
2 1.828 30.471 78.188 1.828 30.471 78.188
3 0.640 10.661 88.848
4 0.478 7.964 96.812
5 0.175 2.920 99.732
6 0.016 0.268 100.000

Table 8

Load matrix of each factor of seedling quality under 0.5% salt concentration"

成分
Component		 发芽率
Germination percentage		 苗高
Seedling height		 苗重
Seedling weight		 根长
Root length		 根数
Root number		 根重
Root weight
1 0.756 0.810 0.720 0.849 0.044 0.050
2 0.490 -0.129 0.014 0.156 0.981 0.987

Table 9

Evaluation coefficient matrix of seedling quality components under 0.5% salt concentration"

成分
Component		 发芽率
Germination percentage		 苗高
Seedling height		 苗重
Seedling weight		 根长
Root length		 根数
Root number		 根重
Root weight
1 0.274 0.356 0.304 0.345 -0.075 -0.073
2 0.159 -0.139 -0.062 -0.800 0.459 0.462

Table 10

Salt tolerance ranking of new rice strains"

品系名称Name of line D值D value 排序Ranking 品系名称Name of line D值D value 排序Ranking
盐粳352 Yanjing 352 0.7575 1 东研稻20 Dongyandao 20 0.5286 60
花粳1520 Huajing 1520 0.7294 2 开734 Kai 734 0.5278 61
盐粳172 Yanjing 172 0.7159 3 盐粳759 Yanjing 759 0.5273 62
沈星稻28 Shenxingdao 28 0.7029 4 G242 0.5269 63
花粳1505 Huajing 1505 0.7009 5 沈星稻4 Shenxingdao 4 0.5263 64
SNR1 0.6992 6 盐粳390 Yanjing 390 0.5236 65
SNR11 0.6961 7 桥选稻161 Qiaoxuandao 161 0.5184 66
花粳1609 Huajing 1609 0.6918 8 花粳14Q6 Huajing 14Q6 0.5165 67
铁1708 Tie 1708 0.6747 9 丹粳优3号 Danjingyou 3 0.5159 68
辽粳169 Liaojing 169 0.6666 10 盐星898 Yanxing 898 0.5155 69
盐粳227 Yanjing 227 0.6611 11 沈稻61 Shendao 61 0.5147 70
阳光60 Yangguang 60 0.6578 12 SNR15 0.5129 71
丹1634 Dan 1634 0.6549 13 SNR9 0.5104 72
花粳1508 Huajing 1508 0.6431 14 沈星稻5 Shenxingdao 5 0.5101 73
铁1702 Tie 1702 0.6422 15 DS821 0.5071 74
盐粳758 Yanjing 758 0.6403 16 G297 0.5058 75
沈星稻11 Shenxingdao 11 0.6351 17 港优7号 Gangyou 7 0.5054 76
盐粳171 Yanjing 171 0.6346 18 G305 0.5054 77
沈农稻513 Shennongdao 513 0.6318 19 辽粳9号 Liaojing 9 (CK) 0.5048 78
盐粳46 Yanjing 46 0.6252 20 沈裕粳1号 Shenyujing 1 0.5033 79
盐粳756 Yanjing 756 0.6225 21 铁166 Tie 166 0.5013 80
花粳1504 Huajing 1504 0.6212 22 SNR10 0.4995 81
阳光48 Yangguang 48 0.6160 23 G233 0.4979 82
盐粳173 Yanjing 173 0.6117 24 SN663 0.4972 83
花粳1624 Huajing 1624 0.6073 25 SNR2 0.4963 84
沈稻68 Shendao 68 0.6063 26 DS861 0.4946 85
沈农稻551 Shennongdao 551 0.6058 27 LDZ16-2 0.4946 86
SNR12 0.6057 28 TL628 0.4937 87
SN1247 0.6053 29 盐星13-8 Yanxing 13-8 0.4928 88
DS798 0.6045 30 佳选稻137 Jiaxuandao 137 0.4913 89
沈稻6号（对照）Shendao 6 (CK) 0.6041 31 佳选稻06 Jiaxuandao 06 0.4866 90
SNR6 0.5998 32 铁165 Tie 165 0.4864 91
D844 0.5988 33 YJP2 0.4859 92
LDC8 0.5931 34 营育2 Yingyu 2 0.4849 93
沈星稻27 Shenxingdao 27 0.5833 35 辽99优27 Liao 99you27 0.4828 94
盐粳39 Yanjing 39 0.5831 36 沈星稻240 Shenxingdao 240 0.4822 95
开513 Kai 513 0.5761 37 丹粳优5号 Danjingyou 5 0.4821 96
沈农稻517 Shennongdao 517 0.5754 38 盐粳403 Yanjing 403 0.4798 97
沈农稻142 Shennongdao 142 0.5748 39 乾稻3号 Qiandao 3 0.4791 98
D11 0.5725 40 G362 0.4720 99
DS901 0.5718 41 营育44 Yingyu 44 0.4717 100
佳选稻139 Jiaxuandao 139 0.5697 42 SNR3 0.4699 101
LDC38 0.5646 43 沈稻97 Shendao 97 0.4698 102
桥选稻172 Qiaoxuandao 172 0.5640 44 SNR8 0.4680 103
沈星稻79 Shenxingdao 79 0.5626 45 SN1101 0.4677 104
YJR32 0.5607 46 TL13 0.4656 105
佳选稻05 Jiaxuandao 05 0.5597 47 铁164 Tie 164 0.4634 106
桥选稻173 Qiaoxuandao 173 0.5522 48 D35 0.4599 107
花粳1611 Huajing 1611 0.5502 49 G169 0.4587 108
LDZ16-1 0.5484 50 SNR7 0.4538 109
盐粳757 Yanjing 757 0.5480 51 SNR4 0.4529 110
SNR13 0.5478 52 YJ1113 0.4490 111
铁163 Tie 163 0.5464 53 铁1712 Tie 1712 0.4449 112
SNR14 0.5449 54 东研稻23 Dongyandao 23 0.4445 113
YJ1232 0.5399 55 桥选稻171 Jiaxuandao 171 0.4340 114
LDC16 0.5388 56 G248 0.4314 115
花粳1607 Huajing 1607 0.5373 57 G278 0.4311 116
SNR5 0.5320 58 辽99优101 Liao 99you101 0.4199 117
桥选稻164 Qiaoxuandao 164 0.5294 59 沈农稻97 Shennongdao 97 0.3771 118
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