Crops ›› 2024, Vol. 40 ›› Issue (3): 180-185.doi: 10.16035/j.issn.1001-7283.2024.03.024

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Physiological and Biochemical Responses of Foxtail Millet to Low Temperature Stress at Seedling Stage

Du Jie(), Su Fuli, Xia Qing, Zhi Hui, Wang Wenxia()   

  1. Department of Life Sciences, Lüliang University, Lüliang 033000, Shanxi, China
  • Received:2023-09-01 Revised:2023-12-21 Online:2024-06-15 Published:2024-06-18

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Abstract:

This study investigated the physiological response mechanism of foxtail millet seedlings to low temperature stress, screening of cold-tolerant germplasm, and provided theoretical basis for the selection and breeding of cold-tolerant cultivar. The cold-tolerant foxtail millet cultivar Jingu 21 and the cold-sensitive foxtail millet cultivar Changnong 48 were used as test materials, and two treatments of low temperature (8 oC) and control (25 oC) were set up, and height, fresh and dry weights, antioxidant enzyme activities and osmoregulatory substances of seedlings under each treatment were determined before the low temperature treatment, after three days of the low temperature treatment and after three days of the recovery treatment, respectively. The results showed that, compared with control, after three days of low-temperature stress treatment in foxtail millet, all the indicators of seedling agronomic traits were significantly reduced, and the reduction of cold-sensitive cultivar was higher than that of cold-tolerant cultivar. After three days of low-temperature stress treatment, SOD, POD and CAT activities of seedling leaves of cold-tolerant cultivar showed increasing trend, and MDA contents increased significantly. The MDA content of cold-sensitive cultivar was significantly higher than that of cold-tolerant cultivar (P < 0.05), and they had stronger protective enzyme activities.

Key words: Foxtail millet, Seedling stage, Low temperature stress, Physiological and biochemical responses

Table 1

Effects of low temperature stress on agronomic characteristics of foxtail millet seedlings"

品种
Cultivar		 处理
Treatment		 苗高
Seedling height (cm)		 鲜重（mg/100株）
Fresh weight (mg/100 plants)		 干重（mg/100株）
Dry weight (mg/100 plants)
JG21 处理前 28.80b 166.44d 24.51g
低温处理3 d后 27.37b 197.08c 37.12d
对照处理3 d后 30.87a 282.83a 41.86b
恢复处理3 d后 28.43b 208.55bc 39.23c
对照恢复3 d后 31.67a 296.85a 44.05a
CN48 处理前 21.87c 152.13e 23.03h
低温处理3 d后 18.70d 166.44d 31.84f
对照处理3 d后 22.60c 197.08c 34.13e
恢复处理3 d后 19.17d 179.50d 33.82e
对照恢复3 d后 23.47c 218.55b 37.69d

Table 2

Effects of low temperature stress on SOD activities of foxtail millet seedlings U/g FW"

品种Cultivar 处理Treatment A B C
JG21 CK 143.19b 138.52b 151.76b
LT 143.19b 221.85a 198.71ab
CN48 CK 125.85b 116.57b 127.30b
LT 125.85b 167.78a 162.15a

Table 3

Effects of low temperature stress on POD activities of foxtail millet seedlings U/(g·min)"

品种Cultivar 处理Treatment A B C
JG21 CK 796.19b 761.73b 745.50b
LT 796.19b 1064.83a 967.19ab
CN48 CK 565.63ab 528.89b 519.36b
LT 565.63ab 671.33a 642.73a

Table 4

Effects of low temperature stress on CAT activities of foxtail millet seedlings μmol/(g·min)"

品种Cultivar 处理Treatment A B C
JG21 CK 358.09b 346.71b 344.50b
LT 358.09b 481.94a 455.21ab
CN48 CK 304.01ab 288.39b 276.71b
LT 304.01b 373.33a 356.67a

Table 5

Effects of low temperature stress on MDA contents of foxtail millet seedlings mmol/g FW"

品种Cultivar 处理Treatment A B C
JG21 CK 7.73b 8.03ab 8.21ab
LT 7.73b 9.34a 9.61a
CN48 CK 8.62b 8.73b 8.92b
LT 8.62b 11.18a 11.74a

Table 6

Effects of low temperature stress on soluble protein contents of foxtail millet seedlings mg/mL"

品种Cultivar 处理Treatment A B C
JG21 CK 12.44b 12.75ab 12.07b
LT 12.44b 14.76a 13.23ab
CN48 CK 7.35b 7.17b 7.64ab
LT 7.35b 8.47a 8.16a
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