Crops ›› 2023, Vol. 39 ›› Issue (2): 83-90.doi: 10.16035/j.issn.1001-7283.2023.02.012

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Regulatory Effects of Water-Nitrogen Coupling on Low Temperature Chilling Damage of Direct-Seeded Early Indica Rice at Seedling Stage

Wang Wenxia1(), Wu Ziming2, Zeng Yongjun2, Pan Xiaohua2, Shi Qinghua2, Zeng Yanhua2()   

  1. 1Department of Life Sciences, Lüliang University, Lüliang 033000, Shanxi, China
    2Key Laboratory of Crop Physiology Ecology and Genetic Breeding of Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China
  • Received:2022-11-12 Revised:2022-11-26 Online:2023-04-15 Published:2023-04-11

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Abstract:

In order to study the control methods of water-nitrogen coupling on low temperature chilling damage of direct-seeded early indica rice at the seedling stage, the cold-tolerant cultivar Xiangzaoxian 6 and cold- sensitive cultivar Zhongjiazao 17 were used as materials, and seven treatments including ambient temperature no nitrogen (CK), low temperature plus no nitrogen (LT-0N), low temperature plus low nitrogen (LT-LN), low temperature plus high nitrogen (LT-HN), low temperature flooding plus no nitrogen (LTF-0N), low temperature flooding plus low nitrogen (LTF-LN) and low temperature flooding plus high nitrogen (LTF-HN) were established for five days to analyse the physiological characteristics such as agronomic characters, antioxidant enzyme activities, osmotic regulation substances, nitrogen metabolism-related enzymes and photosynthase activities, endogenous hormone contents of rice seedlings. The results showed that water-nitrogen coupling treatments could alleviate the injury caused by low temperature chilling damage to direct-seeded early indica rice, compared with LT-LN treatment, the seedling height, root number, maximum root length, fresh weight, dry weight, T1 (the first leaf from top) leaf length and T3 (the 3rd leaf from top) leaf length of cold-sensitive cultivar Zhongjiazao 17 significantly increased after five days of LT-HN treatment; compared with LTF-0N treatment, the root number, root length, fresh weight and T3 leaf length of different tolerant cultivars increased significantly after five days of LTF-LN and LTF-HN treatments, the number of roots and fresh weight were higher in LTF-LN than that in LTF-HN. Compared with LT-0N treatment, LT-HN and LTF-LN treatments significantly increased the nitrogen metabolism-related enzymes, photosynthase activities, nitrate nitrogenin, chlorophyll and endogenous growth-promoting hormone contents in leaves of the cold-sensitive cultivar Zhongjiazao 17, but decreased leaf antioxidant enzyme activities, soluble protein, malonic dialdehyde, and endogenous growth- inhibiting hormones contents, high nitrogen and flooding low nitrogen treatments could alleviate low temperature cold damage in seedlings of direct-seeded early indica rice.

Key words: Direct-seeded early indica rice, Low temperature, Seedling stage, Water-nitrogen coupling, Physiological traits

Table 1

Effects of water-nitrogen coupling on agronomic characteristic of direct-seeded early indica rice under low temperature chilling damage condition at the seedling stage"

品种
Variety		 处理
Treatment		 苗高
Seedling
height (cm)		 根数
Root
number		 最大根长
Maximum root
length (cm)		 鲜重
Fresh weight
(mg/100 plants)		 干重
Dry weight
(mg/100 plants)		 叶长Leaf length (cm)
T1 T2 T3
湘早籼6号
Xiangzaoxian 6		 CK 35.2a 12.7b 3.2c 283.3a 48.3b 2.5c 10.2bc 21.3bc
LT-0N 27.4d 9.7d 1.8e 195.7f 38.8d 2.3d 9.3d 19.5d
LT-LN 28.5cd 11.3c 2.3d 235.7de 44.3c 2.5bc 10.7ab 20.8c
LT-HN 30.2bc 13.3ab 2.4d 257.7bc 48.3b 2.6bc 11.0ab 21.6bc
LTF-0N 28.3cd 10.0d 2.4d 220.3e 42.7c 2.6bc 9.7cd 20.8c
LTF-LN 32.1b 14.3a 4.1a 274.7ab 50.5ab 2.8b 11.1a 23.0a
LTF-HN 28.9cd 12.3bc 3.9b 252.0cd 52.9a 3.1a 9.4d 22.2ab
中嘉早17
Zhongjiazao 17		 CK 31.6a 12.0c 3.2ab 275.0ab 43.2bc 2.5bc 9.9bc 19.5bc
LT-0N 25.7d 9.7d 1.6d 231.0d 40.7c 2.0d 8.9d 17.1e
LT-LN 27.8c 13.0bc 2.2c 259.3bc 42.5bc 2.2d 10.4bc 19.1cd
LT-HN 30.3ab 15.0a 2.9b 288.3a 50.9a 2.4bc 11.0ab 20.3b
LTF-0N 28.9bc 11.7c 2.3c 241.3cd 47.7ab 2.4c 9.4cd 18.3d
LTF-LN 31.1a 15.3a 3.2ab 277.7ab 51.3a 2.6ab 10.4bc 19.5bc
LTF-HN 31.3a 14.0ab 3.4a 266.7ab 52.1a 2.7a 11.5a 21.5a
V 2.5 15.5** 13.7** 25.9** 0.2 24.7** 0.0 140.8**
T 24.3** 28.8** 112.5** 31.5** 26.6** 19.3** 11.1** 27.5**
V×T 4.1** 2.0 8.3** 3.0* 3.4* 1.3 5.8** 4.0**

Fig.1

Effects of water-nitrogen coupling on antioxidant enzyme activity and soluble protein content of direct-seeded early indica rice under low-temperature chilling damage condition at the seedling stage Various lowercase letters above the bars mean significant difference at 0.05 probability level, statistical analysis of the same cultivar, the same below"

Fig.2

Effects of water-nitrogen coupling on membrane peroxides of direct-seeded early indica rice under low-temperature chilling damage condition at the seedling stage"

Fig.3

Effects of water-nitrogen on the activities of nitrogen metabolism-related enzymes activity of direct-seeded early indica rice under low-temperature chilling damage condition at the seedling stage"

Fig.4

Effects of water-nitrogen on photosynthetic enzyme activities of direct-seeded early indica rice under low-temperature chilling damage condition at the seedling stage"

Fig.5

Effects of water-nitrogen coupling on endogenous hormone content of direct-seeded early indica rice under low-temperature chilling damage condition at the seedling stage"

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