Crops ›› 2024, Vol. 40 ›› Issue (6): 91-96.doi: 10.16035/j.issn.1001-7283.2024.06.012

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Effects of Low Temperature Stress on Cold-Tolerance of Broomcorn Millet Varieties with Different Cold-Tolerance at Seedling Stage

Dong Yang()   

  1. Qiqihar Sub-Academy of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161006, Heilongjiang, China
  • Received:2023-07-25 Revised:2023-11-28 Online:2024-12-15 Published:2024-12-05

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Abstract:

Using the budding stage highly cold-tolerant resource Bameng Black millet and the highly cold- sensitive resource PAN21 as experimental materials, the effects of low temperature stress on growth indexes, osmotic adjustment and antioxidant capacity of different cold-tolerant millet varieties were compared through a pot experiment under artificial climate chamber conditions. The results showed that under low temperature stress, the dead seedling rate of Bameng Black millet was 8.9%, indicating that it was a strong cold-tolerant millet variety; the dead seedling rate of PAN21 was 80.3%, indicating that it was an extremely cold-sensitive millet variety, which was consistent with their cold-tolerance classification result in the budding stage. The effects of low temperature stress on the aboveground and underground parts of millet were significantly different; the contents of proline, soluble protein, MDA, and H2O2 in millet leaves under low temperature stress were all increased to varying degrees, and the chlorophyll content was reduced. Moreover, by increasing the activities of SOD and POD, the content of ROS was reduced, thereby alleviating the damage to the biological membrane caused by low temperature. The osmotic adjustment substances and the efficiency of antioxidant system are important physiological indexes for measuring the cold-tolerance of broomcorn millet.

Key words: Broomcorn millet, Cold-tolerance, Low temperature stress, Growth index, Physiological index

Table 1

Average dad rates of broomcorn millet seedlings after low temperature stress %"

指标Index BM PAN21
平均死苗率Average dead seedling rate 8.9a 80.3b

Table 2

Effects of low temperature stress on growth indexes of broomcorn millet seedling"

生长指标
Growth index		 巴盟黑糜子胁迫与对照变化量
BM stress and control variation		 PAN21胁迫与对照变化量
PA stress and control variation
3 d 6 d 9 d 变化幅度
Change
(%)		 3 d 6 d 9 d 变化幅度
Change
(%)
苗长Seedling length (mm) 0.000±0.001 -0.034±0.011 -0.058±0.034 -11.81bc -0.007±0.002 -0.061±0.034 -0.167±0.048 -19.54d
根长Root length -0.006±0.003 0.004±0.002 -0.009±0.006 -24.13e -0.013±0.003 -0.034±0.021 -0.088±0.072 -42.71g
苗茎粗Seedling stem width (mm) 0.000±0.000 0.002±0.001 0.000±0.000 -3.27a 0.002±0.002 0.002±0.000 -0.004±0.001 -6.48ab
根粗Root width (mm) -0.002±0.000 -0.006±0.002 -0.023±0.016 -9.12b -0.013±0.005 -0.067±0.055 -0.133±0.110 -17.67d
苗重Seedling weight (mg) 0.003±0.001 -0.024±0.017 -0.067±0.034 -12.79c -0.004±0.003 -0.059±0.021 -0.159±0.053 -27.79e
根重Root weight (mg) 0.005±0.002 0.001±0.001 -0.025±0.013 -18.13d -0.007±0.002 -0.096±.0049 -0.205±0.077 -34.35f

Fig.1

Effects of low temperature stress on proline and soluble protein contents of BM and PAN21 seedlings Different lowercase letters indicate significant differences at P < 0.05 level, the same below."

Fig.2

Effects of low temperature stress on MDA and H2O2 contents of BM and PAN21 seedlings"

Fig.3

Effects of low temperature stress on chlorophyll content of BM and PAN21 seedlings"

Fig.4

Effects of low temperature stress on POD and SOD activities of BM and PAN21 seedlings"

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