Crops ›› 2021, Vol. 37 ›› Issue (1): 118-123.doi: 10.16035/j.issn.1001-7283.2021.01.017

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Effects of Exogenous Spermidine and Glycine Betaine on Seed Germination and Physiological Characteristics of Isatis indigotica Fort. Seedlings under Drought Stress

Han Duohong1,2(), Wang Enjun2, Zhang Yong2, Wang Hongxia2, Wang Yan2, Wang Fu2   

  1. 1School of Life Sciences and Engineering, Hexi University, Zhangye 734000, Gansu, China
    2Key Provincial Laboratory of Universities in Gansu Province for Characteristic Resources Utility in Hexi Corridor, Zhangye 734000, Gansu, China
  • Received:2020-05-14 Revised:2020-11-05 Online:2021-02-15 Published:2021-02-23

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Abstract:

The effects of exogenous spermidine (Spd) and glycine betaine (GB) on osmotic regulators, reactive oxygen species and membrane peroxidation in seed germination and seedling of Isatis indigotica Fort. were studied under 10% PEG drought stress. The results showed that the exogenous Spd (≤75mg/L) and GB (≤30mmol/L) could significantly promote the seed germination of Isatis indigotica Fort. under drought stress. Spd (25-100mg/L) and GB (10-40mmol/L) treatments could improve chlorophyll content, reduce proline content, malondialdehyde accumulation and membrane permeability of seedlings, and effectively alleviate the damage of drought stress. With the addition of exogenous Spd (25-100mg/L) and GB (10-40mmol/L), SOD, CAT and POD activities in seedlings all showed a trend of "increasing first and then decreasing", and reached the maximum value under the T3 treatments (Spd 75mg/L and GB 30mmol/L). Therefore, Spd (75mg/L) and GB (30mmol/L) can effectively reduce the damage of drought stress on seed germination and seedling growth of Isatis indigotica Fort., and improve the drought resistance of seeds and seedlings.

Key words: Spermidine, Glycine betaine, Isatis indigotica Fort., Drought stress, Seed germination, Physiological indexes

Table 1

Concentrations of PEG, exogenous Spd, and exogenous GB"

处理
Treatment		 聚乙二醇+亚精胺
PEG (%)+Spd(mg/L)		 聚乙二醇+甜菜碱
PEG (%)+GB(mmol/L)
CK 蒸馏水 蒸馏水
GH 10+0 10+0
T1 10+25 10+10
T2 10+50 10+20
T3 10+75 10+30
T4 10+100 10+40

Table 2

Effects of exogenous Spd and GB on seed germination of Isatis indigotica Fort. under drought stress"

组合
Combination		 处理
Treatment		 发芽率GR
Germination rate (%)		 发芽势GV
Germination vigor(%)		 发芽指数GI
Germination index		 活力指数VI
Vigor index
PEG+Spd CK 68.89±4.01a 9.67±0.51a 4.48±0.59a 1.15±0.01a
GH 26.67±1.93cd 4.44±1.11b 1.80±0.59b 0.26±0.12c
T1 36.67±5.77bc 6.89±0.22ab 2.58±0.60ab 0.47±0.07bc
T2 42.22±7.29b 8.44±2.47ab 2.61±0.69ab 0.58±0.17bc
T3 45.44±2.92b 10.90±1.23a 3.75±0.69ab 0.86±0.35ab
T4 20.00±1.92d 5.11±0.97b 1.73±0.40b 0.50±0.05bc
PEG+GB CK 68.89±4.01a 9.67±0.51ab 4.48±0.59a 1.15±0.01a
GH 26.67±1.93cd 4.44±1.11d 1.80±0.59d 0.26±0.12e
T1 32.22±11.76bcd 6.33±1.16cd 2.41±0.16c 0.49±0.03d
T2 41.33±1.02bc 8.83±1.40bc 3.03±0.24b 0.66±0.05c
T3 46.67±3.85b 12.45±1.35a 4.15±0.17a 0.96±0.02b
T4 21.11±1.11d 6.20±0.11cd 2.09±0.12cd 0.54±0.04cd

Fig.1

Effects of exogenous Spd and GB on SPAD value of Isatis indigotica Fort. seedlings under drought stress Different letters indicate significant difference (P < 0.05), the same below"

Fig.2

Effects of exogenous Spd and GB on proline content of Isatis indigotica Fort. seedlings under drought stress"

Fig.3

Effects of exogenous Spd and GB on MDA content of Isatis indigotica Fort. seedlings under drought stress"

Fig.4

Effects of exogenous Spd and GB on relative permeability of plasma membrane of Isatis indigotica Fort. seedlings under drought stress"

Fig.5

Effects of exogenous Spd and GB on SOD activity of Isatis indigotica Fort. seedlings under drought stress"

Fig.6

Effects of exogenous Spd and GB on POD activity of Isatis indigotica Fort. seedlings under drought stress"

Fig.7

Effects of exogenous Spd and GB on CAT activity of Isatis indigotica Fort. seedlings under drought stress"

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