大豆抗感资源对大豆胞囊线虫3号生理小种生长发育动态的影响

doi:10.16035/j.issn.1001-7283.2017.01.026

作物杂志,2017, 第1期: 144–149 doi: 10.16035/j.issn.1001-7283.2017.01.026

大豆抗感资源对大豆胞囊线虫3号生理小种生长发育动态的影响

李海燕¹,蔡德利¹,陈井生²,段玉玺³,陈立杰³,商莹宇¹

1黑龙江八一农垦大学农学院,163316,黑龙江大庆
2黑龙江省农业科学院大庆分院,163316,黑龙江大庆
3沈阳农业大学植物保护学院北方线虫研究所,110866,辽宁沈阳

收稿日期:2016-09-27 修回日期:2016-11-04 出版日期:2017-02-15 发布日期:2018-08-26
作者简介:李海燕,教授,主要从事植物病理学教学与研究工作
基金资助:
农业部公益性行业科研专项(201103018);农业部公益性行业科研专项(200903040-03);国家自然科学基金(31171823);国家现代农业产业技术体系(CARS-04-PS13)

The Influence of Resistant and Susceptible Soybean Germplasm on Growth Dynamic Changes of Soybean Cyst Nematode Race 3（SCN3）

Li Haiyan¹,Cai Deli¹,Chen Jingsheng²,Duan Yuxi³,Chen Lijie³,Shang Yingyu¹

1College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163316,Heilongjiang,China
2Daqing Branch of Heilongjiang Academy of Sciences,Daqing 163316,Heilongjiang,China
3Nematology Institute of Northern China,College of Plant Protection,Shenyang Agricultural University,Shenyang 110866,Liaoning,China

Received:2016-09-27 Revised:2016-11-04 Online:2017-02-15 Published:2018-08-26

摘要/Abstract

摘要：

采用苗期盆栽的方法,以五寨黑豆（抗）、07-2-10-6（抗）和合丰35（感）、07-2-7-14（感）为试验材料,测定不同抗性大豆对胞囊线虫3号生理小种生长发育动态的影响。结果表明,五寨黑豆及07-2-10-6根系分泌物能抑制线虫卵的孵化,而合丰35及07-2-7-14根系分泌物能刺激线虫卵的孵化。培养10d后,合丰35及感病后代07-2-7-14的线虫卵孵化相对值分别为3.71和4.38,五寨黑豆及抗病后代07-2-10-6的线虫卵孵化相对值分别为0.89和1.39。大豆根内各龄线虫数量的动态变化结果表明,侵入抗病品种中的J2幼虫数量较少,只有部分J2幼虫可以进一步发育成高龄期的线虫,且发育速度迟缓,雌雄比值为1∶4.13;侵入感病品种中的J2幼虫群体数量大,多数能够正常发育成更高一级的虫体,发育速度明显大于抗病品种中的J2幼虫,雌雄比值为10.56∶1。五寨黑豆抗大豆胞囊线虫是其根外抗性、抗发育和抗繁殖多种抗病机制综合作用的结果。

关键词: 大豆抗感资源, 大豆胞囊线虫, 3号生理小种, 根分泌物, 发育动态

Abstract:

A pot experiment was conducted with resistant cultivars Wuzhai Heidou, 07-2-10-6 and susceptible cultivars Hefeng 35, 07-2-7-14. The influence of different germplasm to growth dynamic changes of soybean cyst nematode race 3（SCN3）were determined. The results indicated that root exudates had an important effect on the egg hatch of SCN3. Root exudates of Hefeng 35 and 07-2-7-14 stimulated egg incubation of SCN3, while root exudates of Wuzhai Heidou and 07-2-10-6 inhibited egg incubation of SCN 3. After incubating 10 days, the J2 hatching rate of Hefeng 35, 07-2-7-14 were 3.71 and 4.38, respectively, while the J2 hatching rate of Wuzhai Heidou, 07-2-10-6 were 0.89 and 1.39, respectively. The dynamic changes of SCN3 in soybean root indicated that the number of J2 seldom invaded into resistant cultivars. There was only a part of larva at J2 stage that could develop to J3 stage, and their growth was delayed that the ratio of female to male was 1:4.13. But there was a large number of J2 stage larva that invaded into susceptible cultivars, and most of them could develop to J3 stage. The growth speed of larva in susceptible cultivars was faster than that in resistant cultivars, and the ratio of female to male was 10.56:1. The resistant mechanism of Wuzhai Heidou to SCN 3 was integrated effects of anti-intrusion, anti-growth and anti-reproduction.

Key words: Resistant and susceptible soybean germplasm, Soybean cyst nematode, Race 3 (SCN3), Root exudates, Growth dynamic changes

李海燕,蔡德利,陈井生,段玉玺,陈立杰,商莹宇. 大豆抗感资源对大豆胞囊线虫3号生理小种生长发育动态的影响[J]. 作物杂志, 2017 (1): 144–149

Haiyan Li,Deli Cai,Jingsheng Chen,Yuxi Duan,Lijie Chen,Yingyu Shang. The Influence of Resistant and Susceptible Soybean Germplasm on Growth Dynamic Changes of Soybean Cyst Nematode Race 3（SCN3）[J]. Crops, 2017(1): 144–149

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参考文献 23

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品种（系） Varieties (lines)	苗龄Age seedling
品种（系） Varieties (lines)	6d	18d	30d
五寨黑豆Wuzhai Heidou	169d	174b	134c
07-2-10-6	262c	303b	313b
合丰35 Hefeng 35	697b	1 221a	1 364a
07-2-7-14	824a	1 033a	1 432a

品种（系） Varieties(lines)	6d			12d			18d			24d			30d
品种（系） Varieties(lines)	J2	J3	J4	J2	J3	J4	J2	J3	J4	J2	J3	J4	J2	J3	J4
五寨黑豆Wuzhai Heidou	14.7c	0.0c	0.0	39.6d	5.7d	0.0c	29.7b	14.3c	6.9d	10.4a	0.0c	17.1bc	7.3a	0.0	3.3a
合丰35 Hefeng 35	80.7b	13.4a	1.3	161.3b	31.3b	10.7ab	33.7ab	39.7b	56.3c	0.0c	3.3b	22.1b	5.3b	0.0	0.0b
07-2-10-6	53.7b	0.0c	0.0	80.6c	19.7c	6.7b	41.3a	35.3b	17.7b	3.6b	8.7a	10.0c	0.0c	0.0	3.7a
07-2-7-14	111.4a	6.1b	0.0	201.5a	47.7a	13.2a	25.5b	67.8a	73.9a	0.0c	0.0c	33.7a	0.0c	0.0	0.0b

大豆抗感资源对大豆胞囊线虫3号生理小种生长发育动态的影响

The Influence of Resistant and Susceptible Soybean Germplasm on Growth Dynamic Changes of Soybean Cyst Nematode Race 3（SCN3）

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品种（系）Varieties(lines)	I	II	III	平均值The average value
合丰35 Hefeng35	42	66	49	52.3±7.13aA
07-2-7-14	38	58	42	46.0±6.11aAB
07-2-10-6	15	32	16	21.0±5.51bAB
五寨黑豆Wuzhai Heidou	15	16	13	14.7±0.88bB

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