6-BA浸种对盐胁迫下绿豆萌发及幼苗生理特性的影响

doi:10.16035/j.issn.1001-7283.2018.01.027

摘要/Abstract

摘要：

6-苄氨基腺嘌呤（6-BA）是一种人工合成的细胞分裂素,可促进种子萌发,延缓花及果实衰老。本试验以晋绿9号为材料,用不同浓度的6-BA浸种,探究6-BA 对盐胁迫下绿豆种子萌发和幼苗生理生化指标的影响。结果表明：（1）低浓度6-BA（≤10mg/L）处理可缓解盐胁迫对种子萌发的影响,提高绿豆种子的发芽势和发芽率。当提高6-BA的浸种浓度（≥20mg/L）,种子萌发受到抑制;（2）低浓度盐胁迫会促进根和幼苗下胚轴的伸长,高浓度盐胁迫则抑制根的生长;6-BA浸种会抑制根和幼苗下胚轴的伸长,而且6-BA浓度越高抑制作用越明显;（3）与对照相比,盐胁迫会明显增加绿豆幼苗中脯氨酸（Pro）含量,引起丙二醛

关键词: 绿豆, 6-BA浸种, 盐胁迫, 萌发, 幼苗生理特性

Abstract:

6-benzylaminoadenine (6-BA) is a synthetic cytokinin that promotes seed germination, but slows down flower and fruit aging. The effects of 6-BA on seed germination and physiological and biochemical indexes of mung bean were studied by seed soaking with different concentrations of 6-BA. The results showed that: (1) Low concentration of 6-BA (≤10mg/L) could alleviate the effect of salt stress on seed germination and increase the germination potential and germination rate of mung bean seed. When the soaking concentration of 6-BA (≥20mg/L)was increased, seed germination was inhibited. (2) Low concentration of salt stress will promote the elongation of hypocotyls of roots and seedlings, and the high concentration of salt stress inhibited root growth. 6-BA pretreatment inhibited the elongation of hypocotyls of roots and seedlings, and the higher the concentration of 6-BA, the stronger the inhibition. (3) Compared with the control, salt stress could significantly increase the content of proline (Pro) in mung bean seedlings and cause the increase of malondialdehyde (MDA). but decreased of peroxidase (POD) activity. After pretreatment with low concentration of 6-BA, the contents of Pro and MDA were inhibited under salt stress treatment. Compared with salt stress alone, after high concentration of 6-BA pretreatment, SOD and POD activity of salt stress treatment were increased. The results showed that the seed soaking with suitable concentration 6-BA could promote the germination and seedling growth of mung bean under salt stress.

Key words: Mung bean, 6-BA soaking, Salt stress, Germination, Seedling physiological characteristics

刘建霞,张晓丹,王润梅,周凤,刘文英,刘支平. 6-BA浸种对盐胁迫下绿豆萌发及幼苗生理特性的影响[J]. 作物杂志, 2018 (1): 166–172

Jianxia Liu,Xiaodan Zhang,Runmei Wang,Feng Zhou,Wenying Liu,Zhiping Liu. Effects of Seed Soaking with 6-BA on Germination and Physiological Characteristics of Mung Bean under Salt Stress[J]. Crops, 2018(1): 166–172

图/表 5

图1

图2

图3

图4

图5

参考文献 33

[1]	Kovda V A . Loss of productive land due to salinazation. Ambio-A Journal of the Human Environment, 1983,14(2):91-93.
[2]	王遵亲 . 中国盐渍土. 北京: 科学出版社, 1993: 325-344.
[3]	赵福庚 . 植物逆境生理生态学. 北京: 化学工业出版社, 2004: 137-139.
[4]	谢承陶 . 盐渍土改良原理与作物抗性. 北京: 中国农业科技出版社, 1992.
[5]	李彦, 张英鹏, 孙明 , 等. 盐胁迫对植物的影响及植物耐盐机理研究进展. 植物生理科学, 2008,24(1):258-265. doi: 10.3969/j.issn.1671-0185.2014.03.020
[6]	王淼, 李秋荣, 付士磊 , 等. 外源一氧化氮对干旱胁迫下杨树光合作用的影响. 应用生态学报, 2005,16(2):218-222.
[7]	王忠 . 植物生理学. 北京: 中国农业出版社, 2000: 225-240.
[8]	吕俊, 朱利泉, 沈福 , 等. 6-BA诱导的过氧化氢酶及其在提高水稻抗寒力中的作用研究. 中国农学通报, 2005,21(12):64-66. doi: 10.3969/j.issn.1000-6850.2005.12.019
[9]	Wang Y, Yang Z M, Zhang Q F , et al. Enhanced chilling tolerance in Zoysia matrella by pretreatment with salicylic acid,calcium chloride,hydrogen peroxide or 6-benzylaminopurine. Biologia Plantarum, 2009,53(1):179-182. doi: 10.1007/s10535-009-0030-2
[10]	王三根, 梁颖 . 6-BA对低温下水稻细胞膜系统保护作用的研究. 中国水稻科学, 1995,9(4):223-229.
[11]	Lukatkin A S, Zauralov O A . Exogenous growth regulators as a means of increasing the cold resistance of chilling sensitive plants. Plant Industry, 2009,35(6):384-386.
[12]	任旭琴 . 辣椒耐冷性鉴定与冷适应生理机制研究. 扬州:扬州大学, 2008.
[13]	赵九洲, 汤庚国, 童丽丽 . 水分亏缺下SA和6-BA对大花蕙兰的生理调控效应. 南京林业大学学报(自然科学版), 2004,28(3):27-30. doi: 10.3969/j.issn.1000-2006.2004.03.007
[14]	周红卫, 施国新, 陈景耀 , 等. 6-BA对水花生抗氧化酶系Hg ²+毒害的缓解作用 . 生态学报, 2003,23(2):387-392. doi: 10.3321/j.issn:1000-0933.2003.02.024
[15]	徐莉莉, 李萍, 王玉林 , 等. 细胞分裂素类物质对镉胁迫下玉米幼苗生长和抗氧化酶活性及脯氨酸含量的影响. 环境科学学报, 2010,30(11):2256-2263.
[16]	吴进东, 李金才, 魏凤珍 , 等. 氮肥和6-BA对花后受渍冬小麦抗渍性的调控效应. 西北植物学报, 2012,32(12):2512-2517.
[17]	柳道明, 贾文婕, 王小燕 , 等. 喷施外源6-BA对小麦孕穗期渍害的调控效应. 作物杂志, 2015(2):84-88. doi: 10.16035/j.issn.1001-7283.2015.02.017
[18]	郭彦, 张文会, 魏秀俭 . 6-BA对盐胁迫下大豆幼苗生理指标的影响. 作物杂志, 2006(1):13-15. doi: 10.3969/j.issn.1001-7283.2006.01.005
[19]	Mshra A, Choudhmri M A . Amelioration of lead and mercury affection crmination and rice seed growth by antioxldants. Boilgia Plantarum, 1998,41:469-473. doi: 10.1023/A:1001871015773
[20]	廖祥儒, 朱新产, 万怡震 , 等. 6-BA诱导的带正电荷的葡萄叶过氧化物酶. 植物生理学报, 1999,25(1):87-92. doi: 10.1088/0256-307X/16/12/025
[21]	程须珍 . 中国绿豆产业发展与科技应用论文集. 北京: 中国农业科学技术出版社, 2002: 15-26.
[22]	张秀玲 . 盐胁迫对绿豆种子萌发的影响. 研究简报, 2008(4):52-53.
[23]	连兆煌 . 无土栽培原理与技术. 北京: 中国农业出版社, 2002: 57.
[24]	高俊凤 . 植物生理学实验指导. 北京: 高等教育出版社, 2006: 210-224.
[25]	杨少辉, 季静, 王罡 . 盐胁迫对植物的影响及植物的抗盐机理. 世界科技研究与发展, 2006,28(4):70-76. doi: 10.3969/j.issn.1006-6055.2006.04.012
[26]	温福平, 张檀, 张朝晖 , 等. 赤霉素对盐胁迫抑制水稻种子萌发的缓解作用的蛋白质组分析. 作物学报, 2009,35(3):483-489. doi: 10.3724/SP.J.1006.2009.00483
[27]	尹相博, 李青, 王绍武 . 外源物质缓解盐胁迫下植物幼苗生长的研究进展. 黑龙江农业科学, 2013(11):147-150.
[28]	尚宏芹, 刘兴坦 . 6-BA浸种对HgCl₂胁迫下小麦种子萌发和幼苗生长的影响. 麦类作物学报, 2015,35(10):1438-1444. doi: 10.7606/j.issn.1009-1041.2015.10.17
[29]	李娜, 王琳丹 . 6-BA拮抗脱落酸缓解渗透胁迫对种子萌发的抑制. 植物生理学报, 2014,50(4):389-394.
[30]	杨秀坚, 罗富英 . 不同浓度GA₃、6-BA对萝卜芽苗菜产量影响的研究. 北方园艺, 2006(4):22-23. doi: 10.3969/j.issn.1001-0009.2006.04.008
[31]	王桂芹, 段亚军 . 向日葵不同品种耐盐碱性与解剖结构比较研究. 昭乌达蒙族师专学报, 2002,23(6):34-36.
[32]	郭月玲 . 6-BA对晚直播油菜生长和抗寒性的影响及其作用机理. 南京:南京农业大学, 2007.
[33]	Dhindsa R S, Matowe W . Droght tolerance in two mosses:Correlated with enzymatic defence against lipid peroxidation. Journal of Experimental Botany, 1981,32(1):79-91. doi: 10.1093/jxb/32.1.79

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed