水稻耐盐碱胁迫优异等位变异的发掘

doi:10.16035/j.issn.1001-7283.2018.04.009

摘要/Abstract

摘要：

本研究以281个水稻品种构成的自然群体为试验材料,调查了盐、碱胁迫下水稻种子萌发能力相关性状,选择260对SSR标记对基因组进行扫描,采用Structure软件进行群体分析,利用GLM（General Linear Model）方法对标记与幼苗耐盐碱能力进行关联分析;并以试验材料表型均值为对照,鉴别出携带优异等位变异及载体材料。检测到15个与盐胁迫下种子萌发能力关联的SSR位点,其中第10条染色体上分别与标记RM184和RM171连锁位点的贡献率较大,达到29.51%和31.67%,优异等位变异RM184-211表型效应值最大为0.31,载体材料为‘滇屯502选早’。检测到与碱胁迫下种子萌发能力相关联的20个SSR位点,其中第3染色体与RM168和第4染色体与RM6314连锁位点的贡献率较大,分别达到39.17%和50.02%,优异等位变异RM6314-179表型效应值最大为0.58,载体材料为‘越6(N202)’。

关键词: 水稻, 盐胁迫, 碱胁迫, 萌发能力, 优异等位变异

Abstract:

The genotyping data of 260 SSR markers on representative 281 varieties were used in this study and the index of germination under salt and alkaline stress were surveyed. The association analysis between SSR loci and the index of germination under salt and alkaline stress was performed using GLM software in the version of Tassel 2.0. Elite alleles and their carriedmaterials were determined on the average phenotypic value of all accessions.Out of 15 SSR loci associated with the index of germination under salt stress, RM184 and RM171 had the highest contribution rate 29.51% and 31.67%, respectively. The RM184-211 in variety of 'Diantun 502 xuanzao' had the highest positive phenotypic effect (0.31). Out of 20 SSR loci associated with the index of germination under alkaline stress, RM168 and RM6314 had the highest contribution rate 39.17% and 50.02%, respectively. The RM6314-179 in variety of 'Yue 6(N202)' had the highest positive phenotypic effect (0.58).

Key words: Rice (Oryza sativa L.), Salt stress, Alkaline stress, Germination ability, Elite allele

梁晓宇, 林春雨, 马淑梅, 王洋. 水稻耐盐碱胁迫优异等位变异的发掘[J]. 作物杂志, 2018 (4): 48–52

Xiaoyu Liang, Chunyu Lin, Shumei Ma, Yang Wang. Mining Elite Alleles for Germination Ability in Rice (Oryza sativa L.) under Salt and Alkaline Stress[J]. Crops, 2018(4): 48–52

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K	2	3	4	5	6	7	8
lnP(D)	-81360.3	-69464.2	-60659.4	-53147.1	-49199.0	-42724.4	-38217.2
ΔK	54.10	29.08	47.73	32.46	92.17	22.64	22.04

标记 Marker	染色体 Chromosome	遗传距离 Genetic distance	贡献率(%) Contribution rate	标记 Marker	染色体 Chromosome	遗传距离 Genetic distance	贡献率(%) Contribution rate
RM1358	2	48	6.49	RM72	8	61	17.82
RM3467	3	28	17.92	RM3600	9	63	18.87
RM338	3	62	7.96	RM410	9	79	9.92
RM7403	3	82	15.86	RM184	10	42	29.51
RM5951	4	56	13.56	RM258	10	49	16.39
RM142	4	60	26.71	RM5352	10	71	3.66
RM3170	6	29	15.84	RM171	10	73	31.67
RM3138	6	111	18.05

标记 Marker	染色体 Chromosome	遗传距离 Genetic distance	贡献率(%) Contribution rate	标记 Marker	染色体 Chromosome	遗传距离 Genetic distance	贡献率(%) Contribution rate
RM8059	1	61	19.24	RM7579	6	85	9.67
RM9	1	92	13.37	RM5380	7	67	16.28
RM486	1	154	9.87	RM25	8	52	16.38
RM7	3	37	14.56	RM524	9	43	34.94
RM232	3	77	13.43	RM3533	9	65	13.39
RM168	3	123	39.17	RM184	10	42	22.61
RM16	3	132	7.24	RM1125	10	47	23.04
RM6314	4	42	50.02	RM6100	10	54	4.47
RM471	4	54	18.61	RM6327	11	2	44.90
RM161	5	97	25.43	RM6544	11	20	2.70

位点-等位变异 Locus-allele	表型效应值 Phenotypic effect value	载体品种 Carried variety	位点-等位变异 Locus-allele	表型效应值 Phenotypic effect value	载体品种 Carried variety
RM1358-152	0.16	越76(7133)	RM184-211	0.31	滇屯502选早
RM3467-89	0.28	聚子光	RM258-149	0.28	越7(TBR1)
RM3467-121	0.28	补血糯	RM5951-57	0.24	垦稻12
RM3467-127	0.28	香珠糯	RM142-206	0.26	南农粳3786
RM338-177	0.17	越18(AYTO1-D12)	RM142-238	0.26	龙沟种
RM7403-248	0.06	帽子头	RM3170-180	0.29	滇屯502选早
RM3138-95	0.27	越33(8603)	RM3600-96	0.27	三百粒头
RM72-157	0.27	越6(N202)	RM5352-130	0.08	南农粳62401
RM410-177	0.10	千斤稻	RM171-280	0.17	帽子头

位点-等位变异 Locus-allele	表型效应值 Phenotypic effect value	载体品种 Carried variety	位点-等位变异 Locus-allele	表型效应值 Phenotypic effect value	载体品种 Carried variety
RM8095-109	0.51	越21(94-3-1)	RM6544-145	0.29	越21(94-3-1)
RM9-218	0.51	越49(AC5)	RM168-83	0.51	越37(2)
RM486-114	0.49	越23(LCV10)	RM16-178	0.51	越108(73)
RM7-180	0.36	南农粳62401	RM6314-179	0.58	越6(N202)
RM233-145	0.52	越21(94-3-1)	RM25-160	0.52	越61(D19)
RM471-116	0.46	越57(254)	RM5380-129	0.56	越50(KHAO 85)
RM161-320	0.50	昆农8号	RM524-204	0.48	越87(69)
RM7579-72	0.40	越57(254)	RM3533-223	0.51	越48(D6)
RM184-211	0.39	南农粳62401	RM6100-120	0.40	越54(B0T1)
RM1125-99	0.53	越38(70)	RM6327-172	0.44	越21(94-3-1)