水稻潜根线虫生防菌筛选、鉴定及其杀线虫活性研究

doi:10.16035/j.issn.1001-7283.2023.02.034

摘要/Abstract

摘要：

水稻潜根线虫在世界各稻区均普遍发生，给水稻生产造成一定的经济损失。从前期对根结线虫有较好毒杀活性的9个菌株中筛选出对水稻潜根线虫有很好毒杀效果的菌株。结果表明，2周发酵液2.5倍稀释液处理水稻潜根线虫，菌株ZW-1和XO-2对潜根线虫毒杀活性较强，72h的死亡率分别为93.7%和96.7%，LC₅₀值分别为90.4451和76.3875μL/mL。根据形态学特征和rDNA-ITS序列分析，鉴定菌株ZW-1和XO-2分别为日本曲霉（Aspergillus japonicus）和黑曲霉（A.niger）。这些生防真菌菌株的发掘，为植物线虫病害的生物防治提供了新的微生物资源。

关键词: 水稻潜根线虫, 生防菌, 筛选, 杀线虫活性

Abstract:

Hirschmanniella sp. is common in all rice regions of the world, which causes some economic losses of rice production. From the nine strains with good virulence against root-knot nematodes, we selected the strains with good virulence against of rice latent root nematodes. The results showed that ZW-1 and XO-2 had high nematocidal activities against Hirschmanniella sp., and the mortality rate in 2.5×dilution for 72h were 93.7% and 96.7%, respectively, with LC₅₀ values of 90.4451 and 76.3875μL/mL, respectively. Strains ZW-1 and XO-2 were identified as Aspergillus japonicus and A.niger, respectively, based on morphological and rDNA-ITS molecular analysis. The discovery of two biocontrol fungal strains provides new microbial resources for the biological control of plant nematode diseases.

Key words: Hirschmanniella sp., Biocontrol fungus, Screening, Nematocidal activity

贾路明, 莫爱素, 莫意雪, 吴海燕. 水稻潜根线虫生防菌筛选、鉴定及其杀线虫活性研究[J]. 作物杂志, 2023 (2): 238–244

Jia Luming, Mo Aisu, Mo Yixue, Wu Haiyan. Screening and Identification of Biocontrol Fungi against Hirschmanniella sp. and Analysis of Nematocidal Activity[J]. Crops, 2023(2): 238–244

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处理 Treatment	处理时间Incubation time
处理 Treatment	24h	48h	72h
2.5倍稀释液2.5 times dilution	0.00±0.00a	0.00±0.00a	0.74±0.74a
水Water	0.00±0.00a	0.00±0.00a	0.00±0.00a

处理时间 Incubation time (h)	发酵液 Fermentation filtrate	斜率 Slope (±SE)	相关系数 Correlation coefficient	LC₅₀（95%置信区间） LC₅₀ (95% CI) (μL/mL)
24	ZW-1	1.7206（±0.2582）	0.9678^**	384.9935（235.8036~628.5741）
	XO-2	1.3485（±0.1992）	0.9688^**	447.8656（266.6069~752.3573）
48	ZW-1	2.3146（±0.3341）	0.9701^**	136.5220（102.0527~182.6336）
	XO-2	2.3538（±0.2537）	0.9923^***	128.9058（111.7788~148.6570）
72	ZW-1	2.3769（±0.2791）	0.9799^**	90.4451（72.0930~113.4688）
	XO-2	2.6327（±0.1341）	0.9961^***	76.3875（69.0175~84.5446）