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作物杂志,2023, 第6期: 160–166 doi: 10.16035/j.issn.1001-7283.2023.06.022

• 生理生化·植物营养·栽培耕作 • 上一篇    下一篇

外源茉莉酸对小白菜耐硒性及硒积累的作用研究

陈锦平(), 潘丽萍, 邢颖, 廖青, 刘永贤, 车江旅()   

  1. 广西壮族自治区农业科学院农业资源与环境研究所/广西富硒农业研究中心/广西富硒农产品工程技术研究中心，530007，广西南宁
  • 收稿日期:2022-03-18 修回日期:2022-06-07 出版日期:2023-12-15 发布日期:2023-12-15
  • 通讯作者: 车江旅，主要从事蔬菜栽培与育种研究，E-mail：jlvche@163.com
  • 作者简介:陈锦平，主要从事作物微量元素与植物生理响应研究，E-mail：447355175@qq.com
  • 基金资助:
    广西自然科学基金(2020GXNSFBA297014);广西自然科学基金(2020GXNSFAA297086);广西农业科学院项目(桂农科2022JM27);广西农业科学院项目(桂农科2020YT039);广西农业科学院项目(桂农科盟202014)

Study on the Effects of Exogenous Jasmonic Acid on Selenium Tolerance and Selenium Accumulation in Pak Choi (Brassica chinensis L.)

Chen Jinping(), Pan Liping, Xing Ying, Liao Qing, Liu Yongxian, Che Jianglü()   

  1. Agricultural Resources and Environment Research Institute, Guangxi Academy of Agricultural Sciences/ Guangxi Selenium-Rich Agricultural Research Center/Guangxi Selenium-Rich Agricultural Products Engineering Technology Research Center, Nanning 530007, Guangxi, China
  • Received:2022-03-18 Revised:2022-06-07 Online:2023-12-15 Published:2023-12-15

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摘要：

本文研究了不同浓度外源茉莉酸（jasmonic acid，JA，浓度为50μmol/L，100μmol/L）对不同浓度硒酸钠（4mg/kg Se6+，10mg/kg Se6+）处理下小白菜（Brassica chinensis L.）耐硒性和硒积累量的影响。结果表明，土壤富硒处理显著降低小白菜PSII的光化学反应能力并引起强烈的细胞过氧化反应，显著降低地上部生物量。100μmol/L JA处理显著提高富硒处理土壤小白菜的PSⅡ有效量子产量（Fv'/Fm'）、电子传递速率（ETR）、光化学猝灭系数（qP）、地上部生物量和过氧化氢酶（CAT）活性，并显著降低非光化学猝灭系数（qN）和丙二醛（MDA）含量，显著提高富硒处理土壤栽培小白菜的地上部硒含量和硒积累量。50μmol/L JA缓解细胞过氧化和光化学反应抑制效果在4mg/kg Se6+处理下不显著，在10mg/kg Se6+处理下则显著提高最大光化学效率（Fv/Fm）、Fv'/Fm'、ETR和CAT活性，但提高幅度小于100μmol/L JA处理。在富硒土壤下，喷施浓度为100μmol/L的外源茉莉酸可提高小白菜的耐硒性和硒积累能力，在促进富硒土壤中作物健康生长和提高作物硒积累能力上具有良好的应用效果。

关键词: 小白菜, 富硒土壤, 茉莉酸, 叶绿素荧光, 硒积累量

Abstract:

This study examined the effects of varying exogenous jasmonic acid (JA, 50μmol/L, 100μmol/L) concentrations on the tolerance and accumulation of selenium (Se) in pak choi cabbage (Brassica chinensis L.) treated with various selenium (Se) concentrations (4mg/kg Se6+, 10mg/kg Se6+). The results showed that, plants grown in Se-rich soil treatments exhibited Se toxicity, reduced photosynthetic capacity of photosystem (PSII), heavy peroxidization and significant inhibition of shoot biomass. Under Se-rich soil conditions, pak choi cabbage with 100μmol/L JA treatment resisted the effects of Se toxicity. There were significant increases in the effective quantum yield (Fv'/Fm'), electron transport efficiency (ETR), photochemical quenching coefficient (qP), shoot biomass and catalase (CAT) activity, as well as significant reduced non-photochemical quenching coefficient (qN) and malondialdehyde (MDA) content. Additionally, pak choi cabbage cultivated in Se-rich soil and with 100μmol/L JA treatment showed a significant increase in Se content and Se accumulation of shoots. 50μmol/L JA treatment produced varying degrees of Se tolerance dependent on the concentration of soil Se. Pak choi cabbage in 4mg/kg Se6+ treatment showed reductions in cell peroxidation and inhibition of photochemical reaction, though not significantly. While with 10mg/kg Se6+ treatment, there were significant increases in maximum photochemical efficiency (Fv/Fm), Fv'/Fm', ETR, and CAT activity, though not to the degree of the 100μmol/L JA treatment. Application of 100μmol/L exogenous JA on pak choi cabbage can increase Se tolerance and accumulation ability. It is an effective strategy to lessen Se toxicity and enhance crop health in high-Se containing soil.

Key words: Pak choi cabbage, Selenium-rich soil, Jasmonic acid, Chlorophyll fluorescence, Accumulation of selenium

表1

茉莉酸处理对富硒土壤中小白菜叶绿素荧光参数的影响

处理Treatment Fv/Fm Fv'/Fm' ETR qP qN
CK 0.821±0.003abc 0.593±0.005a 118.736±7.324b 0.476±0.032b 0.707±0.029cd
T1 0.812±0.004c 0.533±0.017c 89.960±11.282cd 0.401±0.038c 0.780±0.005a
T2 0.829±0.002a 0.542±0.006bc 86.428±7.198cd 0.379±0.030c 0.784±0.004a
T3 0.818±0.005bc 0.593±0.013a 142.935±12.803a 0.574±0.051a 0.696±0.034d
T4 0.802±0.010d 0.502±0.004d 76.123±4.770d 0.360±0.021c 0.787±0.008a
T5 0.823±0.002ab 0.541±0.008bc 98.313±1.721c 0.432±0.014bc 0.767±0.004ab
T6 0.815±0.002bc 0.561±0.025b 119.038±18.318b 0.504±0.065ab 0.737±0.016bc

图1

茉莉酸处理对富硒土壤中小白菜MDA含量和抗氧化酶活性的影响 不同小写字母表示各处理间在5%水平差异显著；MDA含量，SOD、POD和CAT活性的硒×茉莉酸交互效应的F值依次为1.560、2.904、1.780和5.539，交互效应对CAT活性影响显著（P < 0.05）

表2

茉莉酸处理对富硒土壤小白菜生物量、根冠比及硒含量和硒积累量的影响

处理
Treatment		 地上部生物量
（g/盆）
Shoot biomass
(g/pot)		 根生物量
（g/盆）
Root biomass
(g/pot)		 地上部硒含量
Se content of
shoots (mg/kg)		 根部硒含量
Se content of
roots (mg/kg)		 地上部硒积累量
（μg/盆）
Se accumulation
in shoots (μg/pot)		 根部硒积累量
（μg/盆）
Se accumulation
in roots (μg/pot)		 根冠比
Root-
shoot
ratio
CK 7.17±0.96a 1.74±0.37ab 0.23±0.04e 0.06±0.02d 1.66±0.21d 0.09±0.02e 0.24±0.02a
T1 5.24±0.29b 1.46±0.19b 15.61±2.44d 9.27±1.26c 81.35±8.54c 13.35±0.22d 0.28±0.02a
T2 5.16±0.69b 1.62±0.31ab 17.99±0.73cd 8.56±0.95c 92.57±9.06c 14.10±4.03d 0.32±0.10a
T3 7.04±1.20a 2.01±0.35a 19.92±0.83c 9.42±2.29c 139.60±18.93b 18.48±1.68c 0.29±0.10a
T4 5.17±0.17b 1.78±0.16ab 29.69±0.73b 15.68±1.40b 153.52±2.23b 27.78±1.30b 0.31±0.03a
T5 6.25±0.66ab 1.35±0.05b 37.60±1.76a 20.55±1.45a 234.54±17.88a 27.76±1.08b 0.22±0.03a
T6 7.14±0.06a 1.75±0.27ab 34.98±2.07a 20.87±1.69a 249.93±16.65a 36.15±2.76a 0.24±0.04a
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