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作物杂志,2016, 第4期: 56–61 doi: 10.16035/j.issn.1001-7283.2016.04.009

• 遗传育种·种质资源·生物科技 • 上一篇    下一篇

油用亚麻可溶性糖、脂肪含量与硬脂酰-酰基载体蛋白脱氢酶基因表达相关性分析

王树彦2,韩冰1,周四敏2,徐军3   

  1. 1 内蒙古农业大学生命科学学院,010018,内蒙古呼和浩特
    2 内蒙古农业大学农学院,010018,内蒙古呼和浩特
    3 内蒙古农业大学草原与资源环境学院,010018,内蒙古呼和浩特
  • 收稿日期:2016-05-17 修回日期:2016-06-23 出版日期:2016-08-15 发布日期:2018-08-26
  • 通讯作者: 王树彦
  • 作者简介:作者简介：王树彦,副教授,主要从事作物分子育种方面研究
  • 基金资助:
    内蒙古自治区自然科学基金(2015MS0305);中国科学院西部之光“人才培养”项目

Correlation Analysis between the Expression of Stearoyl Acyl Carrier Protein Dehydrogenase Gene and the Soluble Sugar and Fat Content of Oil Flax

Wang Shuyan2,Han Bing1,Zhou Simin2,Xu Jun3   

  1. 1 College of Life Science,Inner mongolia Agricultural University,Hohhot 010018,Inner Mongolia,China
    2 Agronomy College,Inner mongolia Agricultural University,Hohhot 010018,Inner Mongolia,China
    3 College of Grass Land,Resources and Environment,Inner Mongolia Agricultural University,Hohhot 010018,Inner Mongolia,China
  • Received:2016-05-17 Revised:2016-06-23 Online:2016-08-15 Published:2018-08-26
  • Contact: Shuyan Wang

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摘要：

为研究油用亚麻叶片、果球4个发育阶段硬脂酰-酰基载体蛋白脱氢酶基因（sad）表达水平与可溶性糖含量、脂肪含量之间的关系,以3个已知亚麻酸含量相对不同的油用亚麻品种为试验对象,应用qRT-PCR技术对不同发育时期组织中sad基因的相对表达量与可溶性糖含量以及脂肪含量之间的关系进行分析。结果表明,在油用亚麻叶片和果球的不同发育阶段sad基因的表达模式符合正态分布,在品种和时期之间表达量差异明显。可溶性糖的含量在果球和叶片中差异明显,相关性分析结果显示,在sad基因表达量增加时,可溶性糖含量相对减少;脂肪含量与sad基因的表达量呈极显著正相关。油用亚麻中sad基因表达直接影响油用亚麻可溶性糖和脂肪含量,进而影响亚麻油的品质。

关键词: 油用亚麻, 可溶性糖, 脂肪, 实时荧光定量PCR, 硬脂酰-酰基载体蛋白脱氢酶基因（sad）

Abstract:

To study the relationship between sad gene expression level and the content of soluble sugar, fat content of oil flax leaves and capsules of different developmental stages, three different unsaturated fatty acid content varieties were used to do research with the method of the quantitative real-time fluorescence PCR. The results showed that in oil flax leaves and capsules at different stages of development, expression patterns of sad gene in each period followed the normal distribution, and the difference is significant among varieties and among stages. The soluble sugar content in capsule balls and leaves was significantly different.According to the correlation analysis, with the expression of sad increased, the soluble sugar content decreased. The amount of fat was significantly positively correlated with the expression of sad gene. The expression of sad genes directly influenced soluble sugar, fat content and affected oil quality in oil flax.

Key words: Oil flax, Soluble sugar, Fat, Real-time PCR, Stearoyl acyl carrier protein dehydrogenase gene(sad)

表1

油用亚麻品种与时期的编号"

花后天数(d)
Days after flowering		 叶片Leaves 果球Capules
0 5 15 30 0 5 15 30
Linola AL1 AL2 AL3 AL4 AB1 AB2 AB3 AB4
CDC Gold BL1 BL2 BL3 BL4 BB1 BB2 BB3 BB4
内亚7号Neiya No.7 CL1 CL2 CL3 CL4 CB1 CB2 CB3 CB4

表2

引物序列以及产物大小"

基因
Gene		 方向
Direction		 产物大小(bp)
Product size		 引物序列(5'-3')
Primer sequence
sad F 112 GGACCGCTGAAGAGAATAGG
R CCAGAGCCGATGAGATACTG
apt1 F 110 GTTTATGAATGCGCTTGTCTCA
R TAGAGCTGACCAGGACAAACA

图1

RNA凝胶电泳"

图2

内参基因apt1的引物对4个时期的叶片和果球cDNA检测 对照是DL500 Marker,目的条带为110bp"

图3

各油用亚麻品种叶片、果球中可溶性糖含量的比较 图中不同小写字母表示处理之间差异显著（p<0.05）,下同2.3 脂肪含量分析"

图4

各油用亚麻品种不同部位组织中的脂肪含量"

表3

Pearson相关性分析"

性状
Trait		 sad 可溶性糖含量
Soluble suger content		 脂肪含量
Fat content
sad 1
可溶性糖含量
Soluble suger content		 -0.285* 1
脂肪含量
Fat content		 0.350** -0.324** 1

图5

内参apt1及sad基因熔解曲线"

图6

sad基因的相对表达量"

[1] 赵利, 党占海, 李毅 , 等. 亚麻籽的保健功能和开发利用. 中国油脂, 2006,31(3):71-74.
[2] Broun P, Gettner S, Somerville C . Genetic engineering of plant lipids. Annual Review of Nutrition, 1999,19(1):197-216.
doi: 10.1146/annurev.nutr.19.1.197
[3] Byrne D J, Knauft D A, Shireman R B . Low fat-monounsaturated rich diets containing high-oleic peanuts improve serum lipoprotein profiles. Lipids, 1997,32(7):687-695.
doi: 10.1007/s11745-997-0088-y
[4] 李丹丹, 韩冰, 王树彦 , 等. 亚麻子中α-亚麻酸及参与其形成的不饱和脂肪酸的研究进展. 作物杂志, 2015,165(2):18-22.
[5] Aardra K, John S, Edward W , et al. The Arabidopsis stearoyl-acyl carrier protein-desaturase family and the contribution of leaf isoforms to oleic acid synthesis. Plant Molecular Biology, 2007,63:257-271.
[6] Byfield G E, Xue H, Upchurch R G . Two genes from soybean encoding soluble Δ9-stearoyl-ACP desaturase. Crop Science, 2006,46:840-846.
doi: 10.2135/cropsci2005.06-0172
[7] Florin S, Yael B, Arnon B , et al. Identification and molecular characterization of homeologous Δ9-stearoyl-acyl carrier protein desaturase3 genes from the allotetraploid peanut (Arachis hypogaea). Plant Molecular Biology Reporter, 2010,29:232-241.
[8] Zaborowska Z, Starzycki M, Femiak I , et al. Yellow lupine gene encoding stearoyl-ACP desaturase organization,expression and potential application. Acta Biochimica Polonica, 2002,49:29-42.
[9] Kachroo A, Lapchyk L, Fukushige H , et al. Plastidial fatty acid signaling modulates salicylic acid- and jasmonic acid-mediated defense pathways in the Arabidopsis ssi2 mutant. Plant Cell, 2003,15:2952-2965.
doi: 10.1105/tpc.017301
[10] 王树彦, 李心文 .油用亚麻新品种内亚油1号和内亚油7号的选育.作物杂志,2010(3):21.
[11] Banik M L, Duguid S, Cloutier S . Transcript profiling and gene characterization of three fatty acid desaturase genes in high,moderate,and low linolenic acid genotypes of flax (Linum usitatissimum L.) and their role in linolenic acid accumulation. Genome, 2011,54(6):471-483.
doi: 10.1139/g11-013
[12] 张志良 . 植物生理学实验指导.北京: 高等教育出版社, 2007: 160-162.
[13] 朱振元 .蛹虫草固体饮料加工工艺研究. 天津:天津科技大学, 2014.
[14] Rozen S, Skaletsky H . Primer3 on the WWW for general users and for biologist programmers. Methods Molecular Biology, 2000,132, 365-386.
[15] Libus J1, Storchová H . Quantification of cDNA generated by reverse transcription of total RNA provides a simple alternative tool for quantitative RT-PCR normalization. Biotechniques, 2006,41(2), 156-158.
doi: 10.2144/000112232
[16] Koch K E . Carbohydrate-modulated gene expression in plants. Annual Review of Plant Physiology and Plant Molecular Biology, 1996,47, 509-540.
doi: 10.1146/annurev.arplant.47.1.509
[17] van Dijken A J, Schluepmann H, Smeekens S C . Arabidopsis trehalose-6-phosphate synthase 1 is essential for normal vegetative growth and transition to flowering. Plant Physiology, 2004,135, 969-977.
doi: 10.1104/pp.104.039743
[18] Gutiérrez J C 1, Barquet L A, Caminal J M , et al. Intravitreal bevacizumab (avastin) in the treatment of macular edema secondary to retinal vein occlusion. Clin Ophthalmology, 2008,2(4):787-791.
[19] Udvardi M K, Czechowski T, Scheible W R . Eleven golden rules of quantitative RT-PCR. Plant Cell, 2008,20(7), 1736-1737.
doi: 10.1105/tpc.108.061143
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