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作物杂志,2017, 第3期: 69–74 doi: 10.16035/j.issn.1001-7283.2017.03.013

• 生理生化·植物营养·栽培耕作 • 上一篇    下一篇

箭筈豌豆镉胁迫下的失水胁迫和渗透调节物质的积累

芮海云1,2,张兴兴2,沈振国2,张芬琴3   

  1. 1 泰州学院医药与化学化工学院,225300,江苏泰州
    3 南京农业大学生命科学学院,210095,江苏南京
    3 河西学院农业与生物技术学院,734000,甘肃张掖
  • 收稿日期:2017-04-11 修回日期:2017-04-27 出版日期:2017-06-15 发布日期:2018-08-26
  • 作者简介:芮海云,副教授,研究方向为植物环境生理
  • 基金资助:
    国家自然科学基金(31160053);泰州学院博士基金(TZXY2015JBJJ005)

Water Deficit Stress and Osmotic Substances Accumulation of Vicia sativa L. under Cadmium Stress

Rui Haiyun1,2,Zhang Xingxing2,Shen Zhenguo2,Zhang Fenqin3   

  1. 1 College of Pharmacy and Chemistry & Chemical Engineering,Taizhou University,Taizhou 225300,Jiangsu,China;
    2 College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China
    3 College of Agriculture and Biotechnology,Hexi University,Zhangye 734000,Gansu,China
  • Received:2017-04-11 Revised:2017-04-27 Online:2017-06-15 Published:2018-08-26

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摘要：

通过溶液培养试验,研究了2个箭筈豌豆(Vicia sativa L.)品种(镉耐性品种L3,镉敏感品种ZM)镉胁迫下含水量和渗透调节物质含量的变化,以及2个品种对失水胁迫的耐性。结果表明:25μmol/L镉处理14d,L3和ZM根中含水量分别较对照降低1.27%和4.66%,地上部含水量分别较对照降低4.06%和5.46%,ZM失水更明显;镉胁迫下,ZM和L3根中总氨基酸、脯氨酸和还原糖含量以及茎中总氨基酸含量都较对照显著升高,ZM较L3更明显;ZM叶中脯氨酸和还原糖含量也较对照显著升高,L3叶中二者较对照升高不显著;同时,ZM较L3有更强的对失水胁迫的耐性。因此,镉胁迫下箭筈豌豆渗透调节物质的积累是对镉胁迫引起的失水胁迫的积极响应;箭筈豌豆对失水胁迫的耐性不是决定其镉耐性的主要方面。

关键词: 镉, 箭筈豌豆, 失水胁迫, 渗透调节

Abstract:

We investigated the effects of cadmium stress on water content and osmotic substances accumulation in two Vicia sativa varieties (cadmium-tolerant variety L3, cadmium-sensitive variety ZM), using solution culture experiments, and studied the tolerance of two varieties to water deficit stress. The results showed that water content decreased 1.27% and 4.66% in the roots, and 4.06% and 5.46% in the overground part of L3 and ZM, respectively. Content of total amino acid, proline and reducing sugar increased in the roots by two varieties and content of total amino acid increased in the stems by two varieties under cadmium stress, and the changes were more significant in ZM than in L3; Content of proline and reducing sugar increased significantly in the leaf of ZM but not in the leaf of L3. Meanwhile, Cd-sensitive variety ZM was more tolerant to water deficit stress than Cd-tolerant variety L3. The results indicated that accumulation of osmotic substances in V. sativa was a positive response to water deficit caused by cadmium stress. The tolerance to water deficit stress was not the main factor influencing the cadmium tolerance of V. sativa.

Key words: Cadmium, Vicia sativa L., Water deficit stess, Osmotic adjustment

图1

镉胁迫下2个箭筈豌豆品种根和地上部含水量的变化"

图2

镉胁迫下2个箭筈豌豆品种根、茎、叶总氨基酸含量的变化"

图3

镉胁迫下2个箭筈豌豆品种根、茎、叶脯氨酸含量的变化"

图4

镉胁迫下2个箭筈豌豆品种根、茎、叶还原糖含量的变化"

表1

失水胁迫下2个箭筈豌豆品种生物量的变化"

品种Variety 处理
Treatment		 根长(cm)
Root length		 株高(cm)
Plant height		 根的鲜重(mg/plant)
Root fresh weight		 地上部鲜重(mg/plant)
Overground part fresh weight		 根的干重(mg/plant)
Root dry weight		 地上部干重(mg/plant)
Overground part dry weight
L3 对照Control 30.25±1.10a 38.91±0.74b 314.73±32.28a 463.37±18.57c 16.92±2.20a 51.81±0.45a
10%PEG-6000 22.74±0.45b 35.51±1.47c 253.16±8.86b 402.83±4.62d 17.04±0.31a 46.55±1.02b
ZM 对照Control 31.22±0.13a 44.08±2.02a 299.73±6.01a 565.33±11.85a 15.25±0.52a 54.77±1.06a
10%PEG-6000 20.55±1.74c 41.89±1.85ab 260.06±15.13b 527.03±22.02b 16.29±0.12a 54.48±1.99a
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