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作物杂志,2018, 第6期: 10–16 doi: 10.16035/j.issn.1001-7283.2018.06.002

• 专题综述 • 上一篇    下一篇

马铃薯重要性状QTL定位及3个抗病性状分子标记辅助选育

王伟伟,王洪洋,刘晶,梁静思,李灿辉,唐唯   

  1. 云南师范大学马铃薯科学研究院,650500,云南昆明
  • 收稿日期:2018-05-27 修回日期:2018-08-19 出版日期:2018-12-15 发布日期:2018-12-06
  • 作者简介:王伟伟,硕士研究生,研究方向为马铃薯对晚疫病的抗性机制
  • 基金资助:
    国家自然科学基金(31660503);云南省应用基础研究计划青年项目(2015FD015);全国大学生创新创业计划项目(201710681007)

Quantitative Trait Loci (QTL) Mapping and Three Resistance Traits Linkage Markers Selection in Potatoes

Wang Weiwei,Wang Hongyang,Liu Jing,Liang Jingsi,Li Canhui,Tang Wei   

  1. Joint Academy of Potato Science, Yunnan Normal University, Kunming 650500, Yunnan, China
  • Received:2018-05-27 Revised:2018-08-19 Online:2018-12-15 Published:2018-12-06

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摘要：

马铃薯是我国主要粮食作物之一,马铃薯分子育种研究具有重要意义。在二倍体马铃薯中,重要性状控制基因的QTL定位及克隆已经有大量报道。近年,随着同源四倍体分析软件的开发,四倍体马铃薯的遗传图谱构建和QTL定位也取得了突破性进展。分子标记是马铃薯育种的重要辅助手段,可快速准确筛选出多个优良性状。对马铃薯重要农艺性状的QTL定位和克隆,以及3个抗病性状分子标记辅助选育的研究进展进行概述,为加快马铃薯分子育种研究提供参考和实践依据。

关键词: 马铃薯, QTL, 分子标记, 育种

Abstract:

Potato is one of the main food crops in China, and research on potato molecular breeding research is of great significance. In the diploid potato, QTL mapping and cloning of important genes has been reported extensively; The development of tetraploid linked analysis software, genetic map construction in tetraploid potato and QTL mapping have also made breakthrough progress in recent years. Meanwhile, molecular markers are an important supplement method for potato breeding and it can quickly and accurately screen out multiple good traits. In this paper, the progress of the QTL mapping, cloning of important agronomic traits in potato and 3 resistance traits linkage markers breeding were summarized, to provid a reference and practical basis for accelerating potato molecular breeding.

Key words: Potato, QTL, Molecular marker, Breeding

表1

马铃薯主效抗晚疫病基因连锁标记"

基因
Gene		 染色体
Chromosome		 来源Origin 标记类型
Marker type
RB[37] 8 S. tuberosum×S. bulbocastanum PCR
Rpi-smiral[39] 11 S. tuberosum PCR
R1[40] 5 S. tuberosum AFLP
R2[41] 4 S. tuberosum PCR
R2[42] 4 S. tuberosum AFLP
R3[35] 11 S. tuberosum RFLP
R6, R7[36] 11 S. tuberosum RFLP
Rpi-ber[43] 10 S. berthaultii ESTs
R11[38] 11 S. tuberosum PCR
Rpi-blb1[44] 8 S. bulbocastanum PCR

表2

马铃薯抗病毒病基因连锁标记"

病毒Virus 抗性基因Resistance gene 来源Origin 染色体Chromosome 标记Mark
PVY Ryadg[48] S. andigena 11 RYSC3/SCAR
PVY Ryadg[48] S. andigena 11 RYSC4/SCAR
PVY Ryadg[49] S. andigena 11 ADG2/CAPs
PVY Ryadg[50] S. andigena 11 PYSC3/PCR based
PVY Rysto[51] S. stoloniferum 12 YES3-3A/STS
PVY Rysto[51] S. stoloniferum 12 YES3-3B/STS
PVY Rysto[51] S. stoloniferum 12 SCARYSTO4/PCR based
PVY Ry-fsto[52] S. stoloniferum 12 GP122718/CAPs
PVY Ry-fsto[53] S. stoloniferum 12 GP122564/CAPs
PVY Rychc[41] S. chacoense 9 Ry186/STS
PVY Rychc[54] S. chacoense 9 38-530/RAPD
PVY Nytbr (HR)[55] S. tuberosum 4 TG506/ RFLP
PVY Ny-1[56] S. tuberosum 9 Sldl1/CAPs
PVY Ny-1[57] S. tuberosum 9 SC8951139/PCR based
PVY Ny-2 (HR)[57] S. tuberosum 11 B11.6/CAPs
PLRV PLRV.1[58] S. chacoense 11 Nl271164/SCAR
PLRV PLRV.2[58] S. chacoense 4 GP76/SCAR
PLRV PLRV.3[58] S. chacoense 5 HM4-26/SCAR
PLRV PLRV.4[59] S. andigena 11 UB864600/SCAR
PLRV Rlretb[60] S. tuberosum 4、9 DMB32-11/CAPs
PLRV Rlretb[60] S. tuberosum 4、9 1367-8a/CAPs
PLRV Rlretb[60] S. tuberosum 4、9 C2-Atlg42990/CAPs
PLRV Rladg[61] S. andigena 5 E35M48.192/AFLP
PLRV Rladg[62] S. andigena 5 RGASC850/SCAR
PVA Naadg (HR)[63] S. andigena 11 GP21/AFLP
PVS Ns (HR)[53] S. andigena 8 SC811-260/CAPs
PVS Ns[64] S. tuberosum 8 CP16/CAPs
PVM Rm[65] S. megistacrolobum 11 GP283-320/CAPs
PVM Rm[65] S. megistacrolobum 11 GP250-510/CAPs
PVM Gm[65] S. gourlayi 9 SC878885/SCAR
PVX Rx[66] S. tuberosum 12 GP34/CAPs
PVX Rx1[67] S. andigena 12 RxSP/STS
PVX Rx1[68] S. andigena 12 CP60/RFLP
PVX Rx2[69] S. acaule 5 GP21/RFLP
PVX Nbtbr (HR)[63] S. tuberosum 5 CT167/RFLP
PVX Nb (HR)[63] S. tuberosum 5 SPUD237/CAPs
PVX Nxphu (HR)[70] S. tuberosum 9 TG424/RELP
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