作物杂志,2020, 第6期: 2329 doi: 10.16035/j.issn.1001-7283.2020.06.004
林淼1(), 张秋芝1, 史利玉1, 刘蓓2, 王红武2, 潘金豹1()
Lin Miao1(), Zhang Qiuzhi1, Shi Liyu1, Liu Bei2, Wang Hongwu2, Pan Jinbao1()
摘要:
48h体外干物质消化率(48h in vitro dry matter digestibility, 48h IVDMD)是衡量青贮玉米品质的重要指标。为了初步探究玉米秸秆消化率的分子遗传机理,以341份玉米自交系为材料,于2018年在沈阳和通辽种植,收获后测定秸秆48h IVDMD。利用全基因组重测序获得的6 276 612个高质量SNPs进行全基因组关联分析,共检测到153个与玉米秸秆消化率显著相关的SNPs位点(P<1.0×10-6),4个SNPs显著水平在P<1.0×10-8以上;共找到38个秸秆消化率的候选基因,主要涉及细胞生长发育、防御反应和信号转导等生物学功能。
[1] | 潘金豹, 张秋芝, 郝玉兰, 等. 我国青贮玉米育种的策略与目标. 玉米科学, 2002,10(4):3-4. |
[2] | Yves B, Carine G, Deborah G, et al. Genetic variation and breeding strategies for improved cell wall digestibility in annual forage crops. A review. Animal Research, 2003,52:193-228. |
[3] | 白琪林, 陈绍江, 董晓玲, 等. 近红外漫反射光谱法测定玉米秸秆体外干物质消化率. 光谱学与光谱分析, 2006,26(2):271-274. |
[4] | 张吉鹍, 邹庆华, 张䴔, 等. 奶牛粗饲料纤维品质的综合评定研究. 中国奶牛, 2009(1):20-22. |
[5] |
Li K, Wang H W, Hu X J, et al. Genetic and quantitative trait locus analysis of cell wall components and forage digestibility in the Zheng58 × HD568 maize RIL population at anthesis stage. Frontiers in Plant Science, 2017,8:1472.
doi: 10.3389/fpls.2017.01472 pmid: 28883827 |
[6] | 王琪. 利用连锁和关联分剖析玉米茎秆细胞壁组分及消化性状的遗传基础. 北京:中国农业科学院, 2015. |
[7] | Wang H W, Han J, Sun W T, et al. Genetic analysis and QTL mapping of stalk digestibility and kernel composition in a high-oil maize mutant (Zea mays L.). Plant Breeding, 2010,129:318-326. |
[8] | Méchin V, Argillier O, Hebert Y, et al. Genetic analysis and QTL mapping of cell wall digestibility and lignification in silage maize. Crop Science, 2001,41:690-670. |
[9] |
Wang H W, Li K, Hu X H, et al. Genome-wide association analysis of forage quality in maize mature stalk. BMC Plant Biology, 2016,16:227.
pmid: 27769176 |
[10] | 杨小红, 严建兵, 郑艳萍, 等. 植物数量性状关联分析研究进展. 作物学报, 2007,33(4):523-530. |
[11] | Huang X Y, Han B. Natural variations and genome-wide association studies in crop plants. annual. review. Plant Biology. 2014,65:531-551. |
[12] | 贺城, 杨增玲, 黄光群, 等. 可见/近红外光谱分析秸秆-煤混燃物的秸秆含量. 农业工程学报, 2013,29(17):188-195. |
[13] | 陈丽丽, 张鹏, 黄新, 等. 玉米基因组DNA提取及浓度测定方法评价. 生物技术通报, 2011(12):70-74. |
[14] |
Li H, Durbin R. Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics, 2009,25(14):1754-1760.
doi: 10.1093/bioinformatics/btp324 pmid: 19451168 |
[15] |
Raj A, Stephens M, Pritchard J K. FastSTRUCTURE:variational inference of population structure in large SNP data sets. Genetics, 2014,197(2):573-589.
doi: 10.1534/genetics.114.164350 |
[16] | 刘晓磊. 一种交替运用固定效应和随机效应模型优化全基因组关联分析的算法开发. 武汉:华中农业大学, 2015. |
[17] |
Wu X, Li K Y, Zheng Y X, et al. Genetic discovery for oil production and quality in sesame. Nature Communications, 2015,6:8609.
doi: 10.1038/ncomms9609 pmid: 26477832 |
[18] |
Barrett J C, Fry B, Maller J, et al. Haploview:Analysis and visualization of LD and haplotype maps. Bioinformatics, 2005,21(2):263-265.
doi: 10.1093/bioinformatics/bth457 pmid: 15297300 |
[19] |
Wang K, Li M H, Hakonarson. ANNOVAR:functional annotation of genetic variants from high-throughput sequencing data. Nucleic Acids Research, 2010,38(16):e164.
doi: 10.1093/nar/gkq603 pmid: 20601685 |
[20] |
Cristina B, Isabel P, Luísa R. Gene expression regulation by upstream open reading frames and human disease. PLoS Genetics, 2013,9(8):e1003529.
doi: 10.1371/journal.pgen.1003529 pmid: 23950723 |
[21] |
McKenna A, Hanna M, Banks E, et al. The Genome Analysis Toolkit:a MapReduce framework for analyzing next-generation DNA sequencing data. Genome Research, 2010,20(9):1297-1303.
pmid: 20644199 |
[22] | 曹运琳, 邢梦云, 徐昌杰, 等. 植物黄酮醇生物合成及其调控研究进展. 园艺学报, 2018,45(1):177-192. |
[23] | 张瑜. 拟南芥FRIGIDA-LIKE蛋白家族成员AtFRL4基因及其上游调控序列的克隆与转化研究. 南京:南京农业大学, 2012. |
[24] | Roe J L, Durfee T, Zupan J R, et al. TOUSLED is a nuclear serine/threonine protein kinase that requires a coiled-coil region for oligomerization and catalytic activity. Journal of Biological Chemistry, 1997,272(9):5838-5845. |
[25] | Mark H L, Lambermon Y F, Lorković Z J. UBA1 and UBA2,two proteins that interact with UBP1,a multifunctional effector of pre-mRNA maturation in plants. Molecular and cell Biology, 2002,22(12):4346-4357. |
[26] |
Pagant S, Bichet A, Sugimoto K, et al. KOBITO1 encodes a novel plasma membrane protein necessary for normal synthesis of cellulose during cell expansion in Arabidopsis. The Plant Cell, 2002,14(9):2001-2013.
doi: 10.1105/tpc.002873 pmid: 12215501 |
[27] | Xin W, Yan J J, Bao C, et al. Glycosyltransferase-like protein ABI8/ELD1/KOB1 promotes Arabidopsis hypocotyl elongation through regulating cellulose biosynthesis. Plant Cell & Environment, 2014,38:411-422. |
[28] | 解敏敏, 晁江涛, 孔英珍. 参与木葡聚糖合成的糖基转移酶基因研究进展. 植物学报, 2015,50(5):644-651. |
[29] |
Chou Y H, Gennady P, Olga A Z. Xyloglucan Xylosyltransferases XXT1,XXT2,and XXT5 and the Glucan Synthase CSLC4 form golgi-localized multiprotein complexes. Plant Physiology, 2012,159(4):1355-1366.
doi: 10.1104/pp.112.199356 pmid: 22665445 |
[30] |
Wang C, Li S, Sophia N, et al. Mutation in xyloglucan 6-xylosytransferase results in abnormal root hair development in Oryza sativa. Journal of Experimental Botany, 2014,65(15):4149-4157.
doi: 10.1093/jxb/eru189 pmid: 24834920 |
[31] |
Vorwerk S, Somerville S, Somerville C. The role of plant cell wall polysaccharide composition in disease resistance. Trends in Plant Science, 2004,9(4):203-209.
doi: 10.1016/j.tplants.2004.02.005 |
[32] |
T. Heitz, D R. Bergey, C A. Ryan. A gene encoding a chloroplast-targeted lipoxygenase in tomato leaves is transiently induced by wounding,systemin,and methyl jasmonate. Plant Physiology, 1997,114(3):1085-1093.
doi: 10.1104/pp.114.3.1085 pmid: 9232884 |
[33] | 宗娜, 阎云花, 王琛柱. 昆虫对植物蛋白酶抑制素的诱导及适应机制. 昆虫学报, 2003(4):533-539. |
[34] |
Chen J, Burke J J, Velten J, et al. FtsH11 protease plays a critical role in Arabidopsis thermotolerance. The Plant Journal, 2006,48(1):73-84.
doi: 10.1111/j.1365-313X.2006.02855.x pmid: 16972866 |
[35] | 陈发晶, 谭蕊, 黄萌雨, 等. 类枯草杆菌蛋白酶在植物和病原物互作中的研究进展. 分子植物育种, 2018,16(10):3146-3153. |
[36] | Albertsen M, Fox T, Leonard A, et al. Cloning and use of the ms9 gene from maize:US20160024520A1. 2015-12-23. |
[37] | 戴毅, 高莹莹, 黄泽峰, 等. 玉米R2R3型转录因子家族生物学功能综述. 江苏农业科学, 2013,41(3):6-8. |
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