褪黑素对干旱胁迫下大豆产量及AsA-GSH循环的影响

doi:10.16035/j.issn.1001-7283.2022.01.026

摘要/Abstract

摘要：

为探究褪黑素对干旱胁迫下大豆抗坏血酸–谷胱甘肽（AsA-GSH）循环的影响,以大豆品种绥农26为材料,采用盆栽称重法,设置3个处理,分别为正常供水（CK）、干旱胁迫（D）和干旱胁迫喷施褪黑素（D+M）。结果表明,与CK处理相比,D处理大豆叶片的超氧阴离子（O₂^-.）和过氧化氢（H₂O₂）含量显著提高,D+M处理相比D处理在干旱后15d时,O₂^-.和H₂O₂含量分别显著下降了18.09%和17.37%。在干旱胁迫后10d时,D+M处理相比D处理AsA和GSH含量分别显著提高了22.39%和15.30%。相比D处理,D+M处理单株粒重显著提高了9.20%。综上所述,外源褪黑素可以提高干旱胁迫下AsA-GSH循环的效率,缓解干旱胁迫对大豆造成的氧化损伤。

关键词: 大豆, 褪黑素, 产量, AsA-GSH循环, 干旱胁迫

Abstract:

In order to investigate the effect of melatonin on soybean AsA-GSH cycle under drought stress, the soybean variety Suinong 26 was used as material, and three treatments were set up by pot weighing method: normal water supply (CK), drought stress (D) and drought stress with spraying melatonin (D+M). The results showed that the contents of O₂^-. and H₂O₂ in soybean leaves under D treatment were significantly increased compared with CK treatment, and the contents of O₂^-. and H₂O₂ on the 15th day after drought under D+M treatment were significantly decreased by 18.09% and 17.37%, respectively. On the 10th day after drought, the contents of AsA and GSH in D+M treatment were significantly increased by 22.39% and 15.30% compared with D treatment, respectively. The grain weight per plant of D+M treatment was significantly higher than that of D treatment, and 9.20% higher than that of D treatment. In conclusion, exogenous melatonin could improve the efficiency of AsA-GSH cycle under drought stress and alleviate the oxidative damage of soybean under drought stress.

Key words: Soybean, Melatonin, Yield, AsA-GSH cycle, Drought stress

杜昕, 李博, 毛鲁枭, 陈伟, 张玉先, 曹亮. 褪黑素对干旱胁迫下大豆产量及AsA-GSH循环的影响[J]. 作物杂志, 2022 (1): 174–178

Du Xin, Li Bo, Mao Luxiao, Chen Wei, Zhang Yuxian, Cao Liang. Effects of Melatonin on Yield and AsA-GSH Cycle in Soybean under Drought Stress[J]. Crops, 2022(1): 174–178

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处理 Treatment	株高 Plant height (cm)	茎粗 Stem diameter (mm)	底荚高 Bottom pod height (cm)
CK	91.45±0.45a	7.20±0.25a	21.50±1.03a
D	87.42±3.12b	6.80±0.21a	19.50±0.70a
D+M	88.99±2.36ab	6.94±0.19a	19.95±1.12a

处理 Treatment	单株荚数 Pod number per plant	单株粒数 Grain number per plant	百粒重 100-seed weight (g)	单株粒重 Grain weight per plant (g)
CK	17.75±0.14a	38.84±0.25a	19.13±0.71a	9.00±0.40a
D	15.99±0.26c	36.26±0.33c	14.75±0.48c	6.23±0.18c
D+M	16.74±0.14b	37.78±0.19b	16.55±0.49b	6.80±0.52b