不同籽粒颜色玉米自交系类胡萝卜素含量分析

doi:10.16035/j.issn.1001-7283.2022.05.009

作物杂志,2022, 第5期: 62–68 doi: 10.16035/j.issn.1001-7283.2022.05.009

• 遗传育种·种质资源·生物技术 • 上一篇下一篇

不同籽粒颜色玉米自交系类胡萝卜素含量分析

朱航¹^,²(), 崔方庆²^,³, 卢传礼², 陈卫卫², 李旭辉², 陆思奇²^,³, 张湘博², 赵华⁴(), 齐永文²^,³()

¹长江大学农学院,434025,湖北荆州
²广东省科学院南繁种业研究所,510316,广东广州
³仲恺农业工程学院农业与生物学院,510100,广东广州
⁴广州国家现代农业产业科技创新中心,510520,广东广州

收稿日期:2022-04-16 修回日期:2022-06-15 出版日期:2022-10-15 发布日期:2022-10-19
通讯作者: 齐永文，研究方向为作物分子育种，E-mail：yongwen2001@126.com; 赵华为共同通信作者，研究方向为农业育种研究及科技推广，E-mail：281277430@qq.com
作者简介:朱航,研究方向为作物分子育种,E-mail： 18209219830@163.com
基金资助:
国家甘蔗产业技术体系(CARS201707);广东省重点领域研发计划(2019B020238001);国家自然科学基金(32072027)

Analysis of Carotenoid Content in Maize Inbred Lines with Different Color Grains

Zhu Hang¹^,²(), Cui Fangqing²^,³, Lu Chuanli², Chen Weiwei², Li Xuhui², Lu Siqi²^,³, Zhang Xiangbo², Zhao Hua⁴(), Qi Yongwen²^,³()

¹College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China
²Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, Guangdong, China
³College of Agriculture & Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510100, Guangdong, China
⁴Guangzhou National Modern Agricultural Industry Science and Technology Innovation Center, Guangzhou 510520, Guangdong, China

Received:2022-04-16 Revised:2022-06-15 Online:2022-10-15 Published:2022-10-19

摘要/Abstract

摘要：

采用高效液相色谱检测60个不同玉米籽粒颜色自交系中类胡萝卜素（叶黄素、玉米黄质、β-隐黄质和β-胡萝卜素）含量,结果显示,叶黄素、玉米黄质、β-隐黄质和β-胡萝卜素的平均含量分别为56.32、0.85、1.31和5.75mg/kg。按籽粒颜色由浅到深,将60个玉米自交系分为4个等级,颜色越深的品种类胡萝卜素含量越高。对R、G、B、Gray值与类胡萝卜素含量进行相关分析表明,R、G、B和Gray值之间存在极显著正相关（P<0.01）,相关系数分别为0.992（G与Gray）和0.939（R与Gray）,4种类胡萝卜素含量间呈极显著正相关,相关系数分别为0.830（β-隐黄质与β-胡萝卜素）和0.815（β-隐黄质与玉米黄质）。根据类胡萝卜素含量对60个玉米自交系进行聚类分析,60个自交系分为5类,其中第1类类胡萝卜素含量最低,第2类属于中富含类胡萝卜素的自交系,第5类属于高维生素A含量的自交系。综上可知,类胡萝卜素的种类及其含量是影响籽粒颜色的重要因素,可为培育高胡萝卜素含量的玉米品种提供依据。

关键词: 玉米, 类胡萝卜素, 高效液相色谱法

Abstract:

The contents of four carotenoids including lutein, zeaxanthin, β-cryptoxanthin and β-carotene in grains of 60 maize inbred lines were detected by high performance liquid chromatography. The results showed that the average contents of lutein, zeaxanthin, β-cryptoxanthin and β-carotene were 56.32, 0.85, 1.31 and 5.75mg/kg, respectively. The 60 maize inbred lines could be classified into four grades according to the grain color from light to dark. The darker the grain color, the higher the carotene content. The correlation analysis of carotenoid contents in 60 maize inbred lines showed that there were extremely significant positive correlations between R, G, B and Gray values (P < 0.01), and the correlation coefficients were 0.992 (between G and Gray) and 0.939 (between R and Gray), respectively. There were extremely significant positive correlations among the contents of four carotenoids, and the correlation coefficients were 0.830 (between β-cryptoxanthin and β-carotene), 0.815 (between β-cryptoxanthin and zeaxanthin) respectively. Cluster analysis was performed according to carotenoid content and the 60 maize inbred lines were divided into five categories. Among them, the first category had the lowest carotenoid content, the second category riched in carotenoids, and the fifth category riched in vitamin A. It was concluded that the types and contents of carotenoids were important factors affecting the color of maize grains, and the conclusion provided a basis for selecting maize with high nutritional value and rich in cryptoxanthin.

Key words: Maize, Carotenoid, High performance liquid chromatography

朱航, 崔方庆, 卢传礼, 陈卫卫, 李旭辉, 陆思奇, 张湘博, 赵华, 齐永文. 不同籽粒颜色玉米自交系类胡萝卜素含量分析[J]. 作物杂志, 2022 (5): 62–68

Zhu Hang, Cui Fangqing, Lu Chuanli, Chen Weiwei, Li Xuhui, Lu Siqi, Zhang Xiangbo, Zhao Hua, Qi Yongwen. Analysis of Carotenoid Content in Maize Inbred Lines with Different Color Grains[J]. Crops, 2022(5): 62–68

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编号 Number	材料名称 Material name	编号 Number	材料名称 Material name	编号 Number	材料名称 Material name	编号 Number	材料名称 Material name	编号 Number	材料名称 Material name
C1	CAU404	C13	CAU204	C25	CAU532	C37	IL18	C49	CAU206
C2	CAU322	C14	CAU366	C26	B73	C38	CAU319	C50	CAU393
C3	CAU440	C15	CAU55	C27	CAU489	C39	CAU75	C51	CAU149
C4	IL23	C16	CAU281	C28	CAU482	C40	CAU146	C52	CAU129
C5	CAU252	C17	CAU219	C29	IL5	C41	CAU164	C53	CAU479
C6	CAU268	C18	CAU140	C30	CAU167	C42	CAU274	C54	CAU261
C7	IL52	C19	CAU597	C31	CAU208	C43	CAU269	C55	IL51
C8	CAU501	C20	CAU338	C32	CAU14	C44	CAU565	C56	IL27
C9	CAU263	C21	CAU513	C33	CAU342	C45	IL86	C57	IL65
C10	CAU159	C22	CAU640	C34	CAU93	C46	CAU502	C58	CAU18
C11	CAU297	C23	CAU525	C35	CAU365	C47	CAU539	C59	CAU160
C12	CAU144	C24	CAU504	C36	CAU416	C48	CAU154	C60	CAU225

标准样品 Standard sample	水平1 Level 1	水平2 Level 2	水平3 Level 3	水平4 Level 4	水平5 Level 5	水平6 Level 6
叶黄素Lutein	200.00	100.00	50.00	25.00	12.50	6.25
玉米黄质Zeaxanthin	200.00	50.00	12.50	3.13	0.78	0.20
β-隐黄质 β-cryptoxanthin	100.00	20.00	4.00	0.80	0.16	0.03
β-胡萝卜素 β-carotene	72.50	36.25	18.13	9.06	4.53	2.27

标准样品Standard sample	线性范围Linear range (mg/kg)	线性方程Linear equation	相关系数Correlation coefficent (r²)
叶黄素Lutein	6.0~200	Y₁=0.015X₁	0.9905
玉米黄质Zeaxanthin	0.0~200	Y₂=0.3023X₂+0.1171	0.9958
β-隐黄质 β-cryptoxanthin	0.0~100	Y₃=0.1119X₃-0.1048	0.9976
β-胡萝卜素 β-carotene	2.0~75	Y₄=0.0023X₄+0.0022	0.9988

标准样品 Standard sample	最大值 Maximum	最小值 Minimum	平均值 Average	方差 Variance
叶黄素Lutein	99.98	0.56	56.32	776.29
玉米黄质Zeaxanthin	2.91	0.02	0.85	0.54
β-隐黄质 β-cryptoxanthin	2.20	0.94	1.31	0.11
β-胡萝卜素 β-carotene	26.61	0.61	5.75	35.57

等级Level	叶黄素Lutein	玉米黄质Zeaxanthin	β-隐黄质β-cryptoxanthin	β-胡萝卜素β-carotene
1	1.69±0.76a	nd	0.94±0.00a	nd
2	50.53±5.44b	0.37±0.08a	1.10±0.03a	2.12±0.40a
3	71.05±3.62b	1.31±0.12b	1.51±0.06b	8.71±1.24b
4	54.76±9.02b	1.35±0.32b	1.56±0.11b	10.89±0.77b
平均值Average	56.32	0.85	1.31	5.75
变异系数Coefficient of variation (%)	49.46	87.06	25.19	103.65
F值F-value	12.291	17.662	17.238	12.767
标准差Standard deviation	27.86	0.74	0.33	5.96

不同籽粒颜色玉米自交系类胡萝卜素含量分析

Analysis of Carotenoid Content in Maize Inbred Lines with Different Color Grains

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项目 Item	R	G	B	Gray	叶黄素 Lutein	玉米黄质 Zeaxanthin	β-隐黄质 β-cryptoxanthin	β-胡萝卜素 β-carotene
R	1.000
G	0.912^**	1.000
B	0.408^**	0.596^**	1.000
Gray	0.939^**	0.992^**	0.632^**	1.000
叶黄素Lutein	-0.256^**	-0.385^**	-0.619^**	-0.402^**	1.000
玉米黄质Zeaxanthin	-0.370^**	-0.520^**	-0.416^**	-0.498^**	0.505^**	1.000
β-隐黄质 β-cryptoxanthin	-0.506^**	-0.598^**	-0.421^**	-0.589^**	0.452^**	0.815^**	1.000
β-胡萝卜素 β-carotene	-0.455^**	-0.532^**	-0.331^**	-0.520^**	0.343^**	0.582^**	0.830^**	1.000

类群Group	叶黄素Lutein	玉米黄质Zeaxanthin	β-隐黄质 β-cryptoxanthin	β-胡萝卜素 β-carotene
1	3.94±1.30	nd	0.94±0.00	nd
2	63.68±2.94	0.83±0.08	1.28±0.03	4.76±0.46
3	24.50±0.00	0.68±0.00	1.32±0.00	15.04±0.00
4	75.44±6.06	1.62±0.22	1.76±0.16	12.41±5.30
5	85.93±4.76	2.82±0.04	2.02±0.06	14.96±3.22
平均值Average	56.76	0.86	1.30	5.19
变异系数Coefficient of variation (%)	49.52	86.05	23.08	94.03
F值F-value	22.87	19.28	20.05	16.29
标准差Standard deviation	28.11	0.74	0.30	4.88

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