Crops ›› 2017, Vol. 33 ›› Issue (1): 107-112.doi: 10.16035/j.issn.1001-7283.2017.01.020

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Effects of Maize Straw Returning with Nitrogen Fertilizer on Soil Enzyme Activity,Microbial Biomass Carbon Content and Respiration

Pang Lidan,Meng Tingting,Zhang Yufei,Ma Yuxuan,Zhou Sen,Wang Fulin,Dai Jianjun   

  1. College of Resources and Environmental Sciences,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
  • Received:2016-10-14 Revised:2016-12-14 Online:2017-02-15 Published:2018-08-26

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Abstract:

The indoors culture method of soil was taken to explore the effects of application of maize straw with urea to soil on soil enzyme activity, microbial biomass carbon and soil respiration amount using 9 treatments including 3 maize straw returning amount and 3 nitrogen application rate levels. Results showed that combined application of maize straw with urea increased soil respiration rate and soil microbial biomass carbon content, and improved soil urease, invertase and catalase activities significantly. The soil microbial biomass carbon content and three kinds of enzyme activities increased with the increase of straw returning amount in the same urea level.The activity of polyphenol oxidase in soil showed a certain inhibition by the application of urea and maize straw. Microbial biomass carbon content, soil urease, invertase, catalase activities were positive correlation significantly, but soil polyphenol oxidase activity was negative correlation with soil respiration rate.

Key words: Maize straw, Nitrogen fertilizer, Soil enzyme, Soil respiration, Microbial biomass carbon

Table 1

Experiment design"

处理
Treatment		 秸秆施入量(t/hm2)
Maize straw application rate		 尿素施入量(kg/t)
Urea application rate
C0N0 0 0
C0N1 0 20
C0N2 0 40
C1N0 9 0
C1N1 9 20
C1N2 9 40
C2N0 4.5 0
C2N1 4.5 20
C2N2 4.5 40

Fig.1

Effects of maize straw with nitrogen fertilizer on soil urease activity"

Fig.2

Effects of maize straw with nitrogen fertilizer on soil PPO activity"

Fig.3

Effects of maize straw with nitrogen fertilizer on soil CAT activity"

Fig.4

Effects of maize straw with nitrogen fertilizer on soil invertase activity"

Fig.5

Effects of maize straw with nitrogen fertilizer on soil microbial biomass carbon content"

Table 2

Total amount of soil respiration after adding maize straw and nitrogen fertilizer CO2-C mg/kg soil"

处理Treatment C0 C1 C2
N0 600.2±27e 3 065.2±63a 2 039.9±81c
N1 858.1±16e 3 001.8±71a 2 937.1±22a
N2 1 505.2±96d 2 653.5±110b 2 028.2±138c

Fig.6

Effects of maize straw with nitrogen fertilizer on soil respiration rate"

Table 3

Correlation analysis of soil enzyme activity, microbial biomass carbon content and total CO2"

相关系数Correlation coefficient CO2总量Total CO2
15d 30d
脲酶活性Urease activity 0.67* 0.64*
蔗糖酶活性Invertase activity 0.84** 0.81**
PPO活性Polyphenol oxidase activity -0.19 -0.26
CAT活性Catalase activity 0.84** 0.75*
微生物量碳含量Microbial biomass carbon content 0.81** 0.68*
[1] 郑立臣, 解宏图, 李维福 , 等. 外源添加物对秸秆还田土壤中无机氮转化影响. 辽宁工程技术大学学报, 2007,11(26):275-277.
[2] 张电学, 韩志卿, 李东坡 , 等. 不同促腐条件下玉米秸秆直接还田的生物学效应研究. 植物营养与肥料学报, 2005,1(6):742-749.
[3] Thormann M N, Currah R S, Bayley S E . Succession of microfungal assemblages in decomposing peatland plants. Plant and Soil, 2003,250(2):323-333.
doi: 10.1023/A:1022845604385
[4] Eskelinen A, Stark S, Mnnist M . Links between plant community composition,soil organic matter quality and microbial communities in contrasting tundra habitats. Oecologia, 2009,161(1):113-123.
doi: 10.1007/s00442-009-1362-5
[5] Nicolardot B, Recous S, Mary B . Simulation of C and N mineralisation during crop residue decomposition: A simple dynamic model based on the C: N ratio of the residues. Plant and Soil, 2001,228(1):83-103.
doi: 10.1023/A:1004813801728
[6] Van Veen J A, Kuikman P J . Soil structural aspects of decomposition of organic matter by micro-organisms. Biogeochemistry, 1990,11(3):213-233.
doi: 10.1007/BF00004497
[7] 于君宝, 刘景双, 王金达 , 等. 不同开垦年限黑土有机碳变化规律. 水土保持学报, 2004,18(1):27-30.
[8] 迟德龙, 刘波 . 谈黑龙江玉米秸秆综合利用. 农机使用与维修, 2015,7(4):27-28.
[9] 方华军, 杨学明, 张晓平 . 东北黑土有机碳储量及其对大气CO2的贡献. 水土保持学报, 2003,17(3):9-12,20.
[10] 董守坤, 刘丽君, 马秀峰 , 等. 秸秆还田对白浆土养分含量的影响.作物杂志, 2011(1):53-55.
[11] 柳敏, 张璐, 宇万太 , 等. 有机物料中有机碳和有机氮的分解进程及分解残留率. 应用生态学报, 2007,18(11):2503-2506.
[12] 关松荫 . 土壤酶及其研究法.北京: 农业出版社, 1986.
[13] 许光辉, 郑洪元 . 土壤微生物分析方法手册.北京: 农业出版社, 1986.
[14] 路文涛, 贾志宽, 张鹏 , 等. 秸秆还田对宁南旱作农田土壤活性有机碳及酶活性的影响. 农业环境科学学报, 2011,30(3):522-528.
[15] 马宁宁, 李天来, 武春成 , 等. 长期施肥对设施菜田土壤酶活性及土壤理化性状的影响. 应用生态学报, 2010,21(7):1766-1771.
[16] 王曙光, 林先贵, 尹睿 , 等. 接种AM真菌对PAEs污染土壤中微生物和酶活性的影响. 生态学杂志, 2004,23(1):48-51.
[17] 陈华癸, 樊庆笙 . 微生物学.北京:农业出版社, 1980.
[18] 王平, 马忠明, 包兴国 , 等. 长期不同施肥方式对小麦/玉米间作土壤蔗糖酶活性的影响. 中国现代化研究, 2009,30(5):611-614.
[19] He Z L, Yang X E, Baligar V C , et al. Microbiological and biochemical indexing systems for assessing acid soil quality. Advances in Agronomy, 2003,78:89-138.
doi: 10.1016/S0065-2113(02)78003-6
[20] Emmerling C, Schloter M, Hartmann A , et al. Functional diversity of soils organisms a review of recent research activities in Germany. Journal of Plant Nutrition and Soil Science, 2002,165:408-420.
doi: 10.1002/1522-2624(200208)165:4<408::AID-JPLN408>3.0.CO;2-3
[21] 孙轶, 魏晶, 吴钢 , 等. 长白山高山冻原土壤呼吸及其影响因子分析. 生态学杂志, 2005,24(6):603-606.
[22] Bandick A K, Dick R P . Field management effects on soil enzyme activities. Soil Biology & Biochemistry, 1999,31:1471-1479.
[23] 武际, 郭熙盛, 鲁剑巍 , 等. 不同水稻栽培模式下小麦秸秆腐解特征及对土壤生物学特性和养分状况的影响. 生态学报, 2013,33(2):565-575.
doi: 10.5846/stxb201111201769
[24] 邱莉萍, 刘军, 和文祥 , 等. 长期培肥对土壤酶活性的影响. 干旱地区农业研究, 2003,21(4):44-47.
[25] 耿玉清, 白翠霞, 赵铁蕊 , 等. 北京八达岭地区土壤酶活性及其与土壤肥力的关系. 北京林业大学学报, 2006,28(5):7-11.
[26] 熊明彪, 田应兵, 雷孝章 , 等. 小麦生长期内土壤养分与土壤酶活性变化及其相关性研究. 水土保持学报, 2003,17(4):27-30.
[27] 马欣, 周连仁, 王晓巍 , 等. 秸秆对根区土壤酶活性、无机氮及呼吸量的影响.中国土壤与肥料, 2012(4):27-33.
[28] 强学彩 . 秸秆还田量的农业生态效应研究. 北京:中国农业大学, 2003.
[29] Schnure J, Rosswall T . Mineralization of nitrogen from 15N labeled fungi,soil microbial biomass and roots and its uptake by barely plant. Plant and Soil, 1987,102:71-78.
doi: 10.1007/BF02370903
[30] 周文新, 陈冬林, 卜毓坚 , 等. 稻草还田对土壤微生物群落功能多样性的影响. 环境科学学报, 2008,28(2):326-330.
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