Crops ›› 2017, Vol. 33 ›› Issue (3): 166-170.doi: 10.16035/j.issn.1001-7283.2017.03.030

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Influence of Environmental Factors on Characteristics of Seed Germination of Badong Angelica pubescens Maxim.

Zhou Chenghe1,Guo Xiaoliang2,Xie Lingling3,Guo Jie2   

  1. 1 Enshi Polytechnic,Enshi 445000,Hubei,China
    2 Institute of Chinese Herbal Medicines,Hubei Academy of Agricultural Sciences/Hubei Innovation Center of Agricultural Science and Technology,Enshi 445000,Hubei,China
    3 Enshi Academy of Agricultural Sciences,Enshi 445000,Hubei,China
  • Received:2017-01-04 Revised:2017-03-17 Online:2017-06-15 Published:2018-08-26

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Abstract:

To explore the germination method of Badong Angelica pubescens Maxim., seeds of Badong Angelica pubescens Maxim. were used to study the influence of temperature, light length, germination bed matrix, soaking method and hormonal factors on germination percentage. Results showed that the germination percentage were significantly influenced by temperature, light length, soaking method and hormonal factors. The optimum germination temperature was 20-25℃. The optimum illumination time was 12h. The optimum seed soaking condition was soaking in the aqueous solution containing 30mg/L IAA and 30mg/L NAA of 20℃ for 12h. The influence of germination substrate on seed germination rate was not significant. This study effectively improved the germination rate of seeds so the amount of seeds would be reduced greatly when seedling which lowered the seedling capitals.

Key words: Badong Angelica pubescens Maxim., Seeds, Germination rate, Environment condition

Table 1

Factors and levels of immersion test"

水平
Level		 75%酒精前处理时间(min)
Time of disinfection
by 75% ethanol		 浸种时间(h)
Soak period		 浸种温度(℃)
Soaking
temperature
1 0 4 20
2 15 8 35
3 30 12 50

Table 2

Factors and levels of hormone treatment mg/L"

水平Level GA 6-BA IAA NAA
1 0 0 0 0
2 300 30 30 30
3 600 60 60 60

Table 3

The germination rate under different germination temperature %"

温度(℃)
Temperature		 重复1
Repeat 1		 重复2
Repeat 2		 重复3
Repeat 3		 均值
Mean
25 17.33 20.67 20.00 19.33Aa
20 23.67 22.00 19.33 21.67Aa
15 10.67 10.00 12.67 11.11Bb

Table 4

The difference of germination rate under different light conditions %"

光照(h/d)
Illumination		 重复1
Repeat 1		 重复2
Repeat 2		 重复3
Repeat 3		 均值
Mean
8
 15.33 14.67 12.00 14.00Ab
12 17.33 17.67 18.67 17.89Aa
16 14.00 17.67 16.33 16.00Aab

Table 5

The difference of germination rate under different germination medium %"

发芽基质
Germination medium		 重复1
Repeat 1		 重复2
Repeat 2		 重复3
Repeat 3		 均值
Mean
滤纸Filter paper 20.00 22.00 18.00 20.00Aa
标准砂Standard sand 23.33 20.67 25.33 22.00Aa
膨胀珍珠岩Expanded perlite 17.33 22.00 22.00 20.44Aa

Table 6

The germination rate under different soaking treatment"

处理编号
Processing number		 75%酒精前处理时间(min)
Time of disinfection by 75% ethanol		 浸种时间(h)
Soak period		 浸种温度(℃)
Soaking temperature		 萌发率Germination rate (%)
1 2 3
1 0 4 20 30.00 34.00 34.00
2 0 8 35 22.00 26.00 30.00
3 0 12 50 26.00 32.00 36.00
4 15 4 35 16.00 16.00 20.00
5 15 8 50 20.00 18.00 24.00
6 15 12 20 20.00 20.00 28.00
7 30 4 50 12.00 20.00 18.00
8 30 8 20 24.00 26.00 32.00
9 30 12 35 24.00 32.00 32.00

Table 7

The range analysis of germination rate under different soaking treatment %"

因素Factors 75%酒精前处理时间
Time of disinfection
by 75% ethanol		 浸种时间
Soak period		 浸种温度
Soaking
temperature
水平1均值
Mean of level 1		 30.00
 22.22
 27.56
水平2均值
Mean of level 2		 20.22
 24.67
 24.22
水平3均值
Mean of level 3		 24.44
 27.78
 22.89
极差
Extreme gap		 9.78
 5.56
 4.67

Table 8

The analysis of variance of germination rate under different soaking treatment"

源Source III型平方和
Type III sum of squares		 df 均方
Mean squares		 F Sig.
校正模型
Correction model		 876.444
 8
 109.556
 8.566
 0.000
截距Intercept 16 725.333 1 16 725.333 1 307.676 0.000
75%酒精前处理时间
Time of disinfection by 75% ethanol		 432.889

 2

 216.444

 16.923

 0.000
浸种时间Soak period 139.556 2 69.778 5.456 0.014
浸种温度
Soaking temperature		 104.000
 2
 52.0001
 4.066
 0.035
误差Error 230.222 18 12.790
总计Aggregate 17 832.000 27
校正的总计
Correction aggregate		 1 106.670
 26

Table 9

The germination rate under different hormone treatment"

处理编号
Processing
number		 GA
(mg/L)		 6-BA
(mg/L)		 IAA
(mg/L)		 NAA
(mg/L)		 萌发率(%)
Germination rate
1 2 3
1 0 0 0 0 30.00 34.00 26.00
2 0 30 30 30 46.00 32.00 46.00
3 0 60 60 60 10.00 8.00 6.00
4 300 0 30 60 30.00 22.00 16.00
5 300 30 60 0 6.00 8.00 8.00
6 300 60 0 30 4.00 0.00 2.00
7 600 0 60 30 36.00 16.00 22.00
8 600 30 0 60 8.00 6.00 4.00
9 600 60 30 0 0.00 0.00 0.00

Table 10

The range analysis of germination rate under different hormone treatment %"

因素Factors GA 6-BA IAA NAA
水平1均值Mean of level 1 26.44 25.78 12.67 12.44
水平2均值Mean of level 2 10.67 18.22 21.33 22.67
水平3均值Mean of level 3 10.22 3.30 13.33 12.22
极差Extreme gap 16.22 22.48 8.66 10.45

Table 11

The analysis of variance of germination rate under different hormone treatment"

源Source III型平方和
Type III sum
of squares		 df 均方
Mean squares		 F Sig.
校正模型
Corrected model		 5 075.556
 10
 507.556
 22.121
 0.000
截距Intercept 6 721.333 1 6 721.333 292.939 0.000
GA 1 536.889 2 768.444 33.492 0.000
6-BA 2 347.556 2 1 173.778 51.157 0.000
IAA 418.667 2 209.333 9.123 0.002
NAA 640.889 2 320.444 13.966 0.000
误差Error 367.111 16 22.944
总计Aggregate 12 164.000 27
校正的总计
Corrected aggregate		 5 442.667
 26
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