Crops ›› 2018, Vol. 34 ›› Issue (6): 76-82.doi: 10.16035/j.issn.1001-7283.2018.06.012

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Effects of Abscisic Acid on Growth and Photosynthetic Characteristics of Maize Seedlings under Low Temperature Stress

Tian Lixin1,Yang Ye1,Zuo Shiyu1,Liu Xuan1,Wei Shi1,Sun Lei2,Li Jing1   

  1. 1 College of Agronomy, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
    2 Institute of Soil Fertilizer and Environment Resources, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
  • Received:2018-06-04 Revised:2018-09-05 Online:2018-12-15 Published:2018-12-06

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Abstract:

Abscisic acid (ABA) is a plant hormone widely used in the physiological and biochemical processes of plants. The effects of ABA on photosynthetic characteristics of maize seedlings under low temperature stress were explored, which provided a theoretical basis for elucidating the defense and protection mechanism of photosynthetic structure of maize seedling leaves under low temperature stress. A pot experiment of maize seedlings (variety Jiulong 5) was conducted to investigate the changes of growth and photosynthetic characteristics of maize seedlings under different concentrations of abscisic acid at 0, 2, 4, 6 and 8 days after low temperature stress. The results showed that, the normal growth of maize seedlings was inhibited significantly under chilling stress. All the decreases in SPAD value, Pn, Tr, Gs, Fv/Fm, Fv/Fo and the increases in Fo reflected the inhibitory effects of chilling stress. Exogenous appropriate concentration of ABA led to a marked increase in Pn, Tr, Gs, Fv/Fm, Fv/Fo and the reduction in Fo of maize seedlings. Therefore, exogenous appropriate concentration of ABA could promote the photosynthesis, PSⅡ reactivity and substance accumulation, as well as enhance the chilling resistance of maize seedlings. Meanwhile, exogenous ABA has a certain concentration effect, that is, “low promotion and high inhibition”. The concentration of 15mg/L ABA in the designed concentration range was the most effective in alleviating the photosynthesis inhibition of chilling stress.

Key words: Maize, Abscisic acid, Chilling stress, Photosynthetic characteristics

Fig.1

Effects of exogenous ABA on the SPAD in the leaves of maize seedlings under chilling stress CK: treatment of clear water and room temperature; C0: treatment of clear water and low temperature; C5: treatment of low temperature and 5mg/L ABA; C15: treatment of low temperature and 15mg/L ABA; C25: treatment of low temperature and 25mg/L ABA; C35: treatment of low temperature and 35mg/L ABA. Different letters above the bar mean significant difference between them (P<0.05). The same below"

Fig.2

Effects of exogenous ABA on the Pn in the leaves of maize seedlings under chilling stress"

Fig.3

Effects of exogenous ABA on the Tr in the leaves of maize seedlings under chilling stress"

Fig.4

Effects of exogenous ABA on the Gs in the leaves of maize seedlings under chilling stress"

Fig.5

Effects of exogenous ABA on the Fo in the leaves of maize seedlings under chilling stress"

Fig.6

Effects of exogenous ABA on the Fv/Fm in the leaves of maize seedlings under chilling stress"

Fig.7

Effects of exogenous ABA on the Fv/Fo in the leaves of maize seedlings under chilling stress"

"

处理
Treatment		 低温胁迫天数Days of chilling stress (d)
0 2 4 6 8
CK 0.35±0.03ab 0.45±0.02ab 0.50±0.04a 0.58±0.03a 0.83±0.03a
C0 0.32±0.03b 0.36±0.09b 0.42±0.01b 0.49±0.02b 0.72±0.02b
C5 0.34±0.03ab 0.41±0.02ab 0.45±0.04ab 0.56±0.02a 0.76±0.05ab
C15 0.38±0.02a 0.45±0.03a 0.48±0.03a 0.57±0.02a 0.82±0.03a
C25 0.34±0.05ab 0.44±0.03a 0.47±0.03ab 0.51±0.03ab 0.77±0.07ab
C35 0.31±0.03b 0.39±0.02ab 0.43±0.02b 0.55±0.02a 0.72±0.02b

Table 2

The correlation analysis between different characters"

相关系数Correlation coefficient SPAD Pn Tr Gs Fo Fv/Fm Fv/Fo 叶片干重Leaf dry weight
SPAD 1
Pn 0.52* 1
Tr 0.64* 0.50 1
Gs 0.60* 0.51* 0.63* 1
Fo -0.65* -0.65* -0.66** -0.62* 1
Fv/Fm 0.76* 0.63* 0.79** 0.69* -0.78** 1
Fv/Fo 0.49* 0.62** 0.57* 0.53* -0.65* 0.70** 1
叶片干重Leaf dry weight -0.37* -0.48* -0.44 -0.43 0.49* -0.46* -0.42 1
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