Crops ›› 2019, Vol. 35 ›› Issue (2): 99-102.doi: 10.16035/j.issn.1001-7283.2019.02.015

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Karyotype Analysis of Chromosome of Sunflower Longkuiza10

Haiyan Ding1,Chunlin Wang1,Yan Wu1,Guoling Ren1,Zuhuan Zhai1,Jun Ma2   

  1. 1 School of Biological Engineering, Daqing Normal University, Daqing 163712, Heilongjiang, China
    2 Industrial Crops Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150080, Heilongjiang, China
  • Received:2018-10-26 Revised:2019-02-18 Online:2019-04-15 Published:2019-04-12
  • Contact: Jun Ma

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Abstract:

In order to analyze the chromosome karyotype and chromosome production techniques of sunflower Longkuiza10, the effects of low temperature pretreatment of sunflower root tip cells production was studied by using sunflower Longkuiza10 as the experimental material. Photoshop and Excel software were used to assist in the analysis of chromosome images. The results showed that the low temperature pretreatment of sunflower root tip cells could obtain more ideal chromosome production for karyotype analysis, and the chromosome number of sunflower Longkuiza10 was 2n=34, and the karyotype formula was 2n=34=30m+2sm+2M.

Key words: Sunflower, Chromosome, Karyotype analysis, Longkuiza10

Fig.1

Model diagram of karyotype of sunflower Longkuiza 10"

Table 1

Karyotype parameters of sunflower Longkuiza10"

编号
Number		 相对长度Relative length (%) 臂比（长臂/短臂）
Ratio (Long arm/Short arm)		 类型
Type
长臂Long arm 短臂Short arm 总长Full length
1 4.4±0.12 3.0±0.16 7.4±0.25 1.47±0.06 m
2 4.4±0.24 2.6±0.14 7.0±0.35 1.69±0.09 m
3 4.0±0.10 2.8±0.16 6.8±0.21 1.43±0.08 m
4 3.8±0.16 2.8±0.10 6.6±0.19 1.36±0.08 m
5 4.4±0.07 2.0±0.07 6.4±0.10 2.20±0.08 sm
6 3.2±0.12 3.2±0.07 6.4±0.14 1.00±0.04 M
7 3.6±0.07 2.4±0.10 6.0±0.07 1.50±0.09 m
8 3.2±0.16 2.8±0.07 6.0±0.21 1.14±0.04 m
9 3.6±0.16 2.2±0.10 5.8±0.10 1.64±0.14 m
10 3.2±0.14 2.6±0.07 5.8±0.12 1.23±0.08 m
11 3.6±0.16 2.0±0.07 5.6±0.20 1.80±0.08 m
12 3.0±0.07 2.4±0.10 5.4±0.07 1.25±0.08 m
13 2.8±0.16 2.6±0.16 5.4±0.24 1.08±0.08 m
14 2.8±0.12 2.4±0.19 5.2±0.26 1.17±0.09 m
15 2.8±0.07 2.4±0.07 5.2±0.12 1.17±0.03 m
16 2.6±0.14 2.0±0.07 4.6±0.19 1.30±0.06 m
17 2.4±0.16 2.0±0.07 4.4±0.20 1.20±0.07 m
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