Crops ›› 2020, Vol. 36 ›› Issue (3): 53-59.doi: 10.16035/j.issn.1001-7283.2020.03.009

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The Difference of Seed Germination in Different Cotton Genotypes in Response to Copper Stress

Yuan Changkai, Luo Haihua, Chen Gong, Gao Xin, Peng Jinjian, Xiang Chunling, Yin Mengyao, Wang Peipei, Xu Lanlan, Tang Feiyu()   

  1. College of Agronomy, Jiangxi Agricultural University/Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Nanchang 330045, Jiangxi, China
  • Received:2019-11-11 Revised:2020-03-01 Online:2020-06-15 Published:2020-06-10
  • Contact: Feiyu Tang E-mail:fytangcau@163.com

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Abstract:

It is of great significance for increasing the usage of cultivated land and the income of cotton producers to grow cotton in the copper-polluted soil, but the effect of copper stress on the germination of cotton seeds remains unclear. Seven cotton varieties (lines) were evaluated to a seed germination experiment where seeds were incubated in the solution with four different Cu2+ concentration (0, 10, 100 and 2 000μmol/L). The germination rate, seedling fresh weight, root length and shoot length were recorded on ten days after seed incubation. The tolerance index and subordinate function analyses were applied to assess the difference in tolerance capacity to copper toxicity among seven cotton genotypes. The results showed that seedling fresh weight, root length and shoot length decreased with the increasing of Cu2+ concentration over the range of 10 to 2 000μmol/L. The reduction of root length due to copper stress was greater than that of shoot length. The tolerance ability to copper stress of seed germination could be well distinguished at low-middle ion levels among these genotypes. Among the seven cotton varieties (lines), Ekangmian 10 ranked first and SGK321 at the end in the terms of tolerance index and subordinate function value. It has been concluded that Ekangmian 10 is a copper resistant genotype and SGK321 is a sensitive genotype to copper stress.

Key words: Cotton (Gossypium hirsutum L.), Copper stress, Seed germination, Tolerance index, Subordinate function

Table 1

Effects of copper stress on seed germination percentage in different cotton genotypes %"

Cu2+浓度
Cu2+ concentration
(μmol/L)		 2870 鲁7619
Lu 7619		 鲁301
Lu 301		 A409 CN01 鄂抗棉10号Ekangmian 10 SGK321
GP IGP GP IGP GP IGP GP IGP GP IGP GP IGP GP IGP
0 55a - 76a - 35a - 83a - 45b - 69a - 61b -
10 49a 11.36 73a 4.69 40a -14.29 81a 1.52 35b 22.22 40b 41.82 63b -2.04
100 46a 15.91 83a -8.33 50a -42.86 83a 0.00 45b 0.00 48b 29.70 63b -3.40
2 000 58a -4.55 83a -8.33 40a -14.29 74a 10.61 71a -58.33 51b 25.45 74a -20.41

Table 2

Effects of copper stress on the seedling fresh weight in different cotton genotypes g"

Cu2+浓度
Cu2+ concentration (μmol/L)		 2870 鲁7619
Lu 7619		 鲁301
Lu 301		 A409 CN01 鄂抗棉10号
Ekangmian 10		 SGK321
0 0.6342a 0.6258a 0.7158a 0.6153a 0.5897a 0.6298a 0.6220a
10 0.5452b 0.4675b 0.5722b 0.5805a 0.5665a 0.5760a 0.5035b
100 0.4454c 0.4330b 0.4267c 0.4357b 0.3682b 0.4711b 0.3787c
2 000 0.2819d 0.2802c 0.2226d 0.2651c 0.2641c 0.2953c 0.2577d

Table 3

Effects of copper stress on the root length of seedling in different cotton genotypes"

Cu2+浓度
Cu2+ concentration
(μmol/L)		 2870 鲁7619
Lu 7619		 鲁301
Lu 301		 A409 CN01 鄂抗棉10号
Ekangmian 10		 SGK321
RL
(cm)		 IPRL
(%)		 RL
(cm)		 IPRL
(%)		 RL
(cm)		 IPRL
(%)		 RL
(cm)		 IPRL
(%)		 RL
(cm)		 IPRL
(%)		 RL
(cm)		 IPRL
(%)		 RL
(cm)		 IPRL
(%)
0 7.34a - 8.04a - 8.53a - 7.80a - 6.97a - 7.08a - 10.83a -
10 3.50b 52.3 6.37b 20.8 3.41b 60.1 4.20b 46.1 5.63b 19.3 5.98b 15.5 3.30b 69.5
100 1.90c 74.1 3.99c 50.4 1.95c 77.2 2.00c 74.4 3.83c 45.0 4.17c 41.1 2.09c 80.7
2 000 0.85d 88.4 0.74d 90.8 0.57d 93.3 0.98d 87.4 0.81d 88.4 0.75d 89.4 1.02d 90.5

Table 4

Effects of copper stress on the shoot length of seedling in different cotton genotypes"

Cu2+浓度
Cu2+ concentration
(μmol/L)		 2870 鲁7619
Lu 7619		 鲁301
Lu 301		 A409 CN01 鄂抗棉10号
Ekangmian 10		 SGK321
SL
(cm)		 IPSL
(%)		 SL
(cm)		 IPSL
(%)		 SL
(cm)		 IPSL
(%)		 SL
(cm)		 IPSL
(%)		 SL
(cm)		 IPSL
(%)		 SL
(cm)		 IPSL
(%)		 SL
(cm)		 IPSL
(%)
0 7.45a - 7.90a - 8.70a - 8.63a - 7.78a - 7.37a - 8.16a -
10 6.38b 14.4 6.68b 15.5 7.75b 10.8 7.79b 9.7 6.98b 10.2 7.34a 0.4 7.13b 12.5
100 5.44c 26.7 5.75c 27.2 5.68c 34.7 6.38c 18.1 4.95c 36.3 6.12b 16.9 5.37c 34.1
2 000 2.20d 70.5 3.19d 59.6 2.08d 76.0 2.14d 72.4 2.41d 69.0 2.52c 65.8 2.18d 73.3

Table 5

Tolerance indexes of different cotton genotypes at different Cu2+ levels"

基因型Genotype 10μmol/L 100μmol/L 2 000μmol/L
幼苗鲜重Seedling
fresh weight		 根长
Root
length		 芽长
Shoot
length		 平均
Mean		 排序
Rank		 幼苗鲜重
Seedling
fresh weight		 根长
Root
length		 芽长
Shoot
length		 平均
Mean		 排序
Rank		 幼苗鲜重
Seedling
fresh weight		 根长
Root
length		 芽长
Shoot
length		 平均
Mean		 排序
Rank
A409 0.94 0.54 1.07 0.851 3 0.71 0.26 0.82 0.595 4 0.48 0.13 0.28 0.293 3
CN01 0.96 0.81 0.90 0.890 2 0.63 0.55 0.64 0.604 3 0.45 0.12 0.31 0.292 4
鄂抗棉10号
Ekangmian 10		 0.92 0.84 1.00 0.921 1 0.76 0.59 0.83 0.725 1 0.47 0.11 0.34 0.307 2
鲁301 Lu 301 0.80 0.40 0.89 0.697 6 0.60 0.23 0.65 0.493 6 0.31 0.07 0.24 0.206 7
2870 0.86 0.48 0.86 0.731 5 0.70 0.26 0.73 0.565 5 0.44 0.12 0.30 0.285 5
SGK321 0.81 0.30 0.88 0.663 7 0.61 0.19 0.66 0.487 7 0.41 0.09 0.27 0.259 6
鲁7619
Lu 7619		 0.74 0.79 0.85 0.794 4 0.69 0.50 0.73 0.639 2 0.45 0.09 0.40 0.315 1

Table 6

Subordinate function values of different cotton genotypes at different Cu2+ levels"

基因型
Genotype		 10μmol/L 100μmol/L 2 000μmol/L
幼苗鲜重
Seedling
fresh weight		 根长
Root
length		 芽长
Shoot
length		 平均
Mean		 排序
Rank		 幼苗鲜重
Seedling
fresh weight		 根长
Root
length		 芽长
Shoot
length		 平均
Mean		 排序
Rank		 幼苗鲜重
Seedling
fresh weight		 根长
Root
length		 芽长
Shoot
length		 平均
Mean		 排序
Rank
A409 1.00 0.29 1.00 0.765 2 0.65 0.04 1.00 0.565 3 0.98 0.91 0.06 0.649 2
CN01 0.88 0.76 0.31 0.648 3 0.00 0.85 0.00 0.284 6 0.57 0.53 0.30 0.464 6
鄂抗棉10号
Ekangmian 10		 0.96 0.87 0.49 0.775 1 1.00 1.00 0.82 0.941 1 1.00 0.39 0.39 0.594 3
鲁301 Lu 301 0.93 0.03 0.71 0.556 4 0.57 0.02 0.51 0.368 5 0.00 0.00 0.00 0.000 7
2870 0.69 0.06 0.00 0.252 6 0.74 0.00 0.36 0.369 4 0.82 0.62 0.11 0.515 5
SGK321 0.33 0.00 0.39 0.238 7 0.10 0.08 0.29 0.159 7 0.48 1.00 0.08 0.522 4
鲁7619
Lu 7619		 0.00 1.00 0.15 0.384 5 0.63 0.92 0.56 0.703 2 0.79 0.38 1.00 0.723 1
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