Crops ›› 2019, Vol. 35 ›› Issue (6): 187-194.doi: 10.16035/j.issn.1001-7283.2019.06.030

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Effects of Salt Stress on Seeds Germination of Astragalus membranaceus

Ge Zhenmei,Liu Zhiguo,Zhao Lu,Zhang Xiaoyu,Liu Guixia   

  1. College of Life Science, Hebei University, Baoding 071002, Hebei, China
  • Received:2019-06-13 Revised:2019-09-08 Online:2019-12-15 Published:2019-12-11
  • Contact: Guixia Liu

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Abstract:

In order to protect and develop Astragalus membranaceus and improve saline-alkali land, the effects of 3 kinds of salt treatments (NaCl, Na2CO3 and compound salt), the germination rate, germination potential, antioxidant enzyme activity, malondialdehyde (MDA) content, soluble protein content and relative conductivity were analyzed. The results showed that, different salt stress on A. membranaceus seeds has different effects. With the increase of concentration of salt, the germination potential and germination rate of A.membranaceus seed decreased gradually. Low concentration of NaCl and compound salt promoted the activity of antioxidant enzymes, and the inhibition of Na2CO3 against oxidase activity increased with the increase of Na2CO3 concentration. MDA content and conductivity were increased in each treatment. NaCl and compound salt reduced the content of soluble protein in A. membranaceus seeds. The content of soluble protein in Na2CO3 treatment increased with the increase of Na2CO3 concentration. The results showed that the stress effect from strong to weak is Na2CO3 , NaCl, compound salt at the same concentration.

Key words: Astragalus membranaceus, Salt stress, Seed germination

Fig.1

Effects of salt stress on seed germination rate of A. membranaceus Different capital letters indicate the significant difference among different concentration treatments of the same salinity (P<0.05). Different small letters indicate the significant difference among different salinity stress of the same salt concentration (P<0.05). The same below"

Table 1

Effects of salt stress on seed germination of A. membranaceus"

盐分
Salinity		 浓度(%)
Concentration		 发芽势(%)
Germination potential		 发芽指数
Germination index		 萌发活力指数
Germination vitality index
CK 72.0±1.15Ba 28.0±1.46Aa 35.5±2.33Aa
0.3 62.0±1.15Bb 23.8±0.68Ba 32.2±3.06Aa
Na2CO3 0.6 48.7±0.67Cc 18.3±1.77Ca 25.6±5.17ABa
0.9 37.3±2.40Db 12.9±0.90Dc 14.9±1.97Cb
0.3 81.3±1.76Aa 26.0±0.70Aa 35.1±1.00Aa
NaCl 0.6 54.7±0.67Cb 20.3±0.50Ba 28.3±3.80Aa
0.9 44.0±1.15Da 15.8±1.71Cb 20.2±1.83Ba
0.3 65.3±1.33Bb 24.6±0.82ABa 34.3±0.39Aa
复盐Compound salt 0.6 58.7±0.67Ca 20.2±1.67BCa 32.8±1.88Aa
0.9 46.7±1.33Da 18.3±2.13Ca 16.1±1.47Bb

Table 2

Harmfulness and salt tolerance of A. membranaceus seeds under salt stress"

盐分
Salinity		 浓度(%)
Concentration		 耐盐指数
Salt tolerance index		 盐碱害指数(%)
Saline-alkali damage index		 耐盐碱程度
Saline-alkali tolerance		 耐盐碱等级
Saline-alkali tolerance grade
0.3 41.97 14.00±0.02Ca 高耐 1
Na2CO3 0.6 32.84 32.33±0.01Ba 耐 2
0.9 25.14 48.33±0.03Aa 中耐 3
0.3 55.16 -13.00±0.03Cb 高耐 1
NaCl 0.6 37.15 24.00±0.01Bb 耐 2
0.9 29.91 39.00±0.02Ab 耐 2
0.3 44.37 9.33±0.02Ca 高耐 1
复盐Compound salt 0.6 39.83 18.33±0.01Bc 高耐 1
0.9 31.58 35.00±0.02Ab 耐 2

Table 3

Effects of salt stress on seed radicle length, 100-grain fresh weight and SPAD value of A. membranaceus"

盐分Salinity 浓度Concentration (%) 胚根长Radicle length (cm) 百粒鲜重100-grain fresh weight (g) SPAD值SPAD value
CK 2.6±0.05Aa 1.42±0.11Aa 27.94±1.91Aa
0.3 1.9±0.07Bb 1.24±0.08ABa 21.19±3.24Ba
Na2CO3 0.6 1.2±0.17Cc 1.22±0.02ABa 13.24±1.54Ca
0.9 0.8±0.08Cd 1.02±0.10Bb 8.12±0.96Db
0.3 2.9±0.05Aa 1.35±0.07Aa 21.66±1.67Ba
NaCl 0.6 2.1±0.10Ba 1.27±0.01Aa 18.15±2.92BCa
0.9 1.7±0.05Ca 1.24±0.05Aa 15.19±1.64Ca
0.3 2.4±0.05Ab 1.37±0.04ABa 23.55±1.53Aa
复盐Compound salt 0.6 2.2±0.09Ba 1.26±0.04ABa 16.85±1.41Ba
0.9 1.4±0.04Cb 1.18±0.04Bab 10.58±1.32Cb

Fig.2

Effects of salt stress on the SOD activity of A. membranaceus seeds"

Fig.3

Effects of salt stress on the POD activity of A. membranaceus seeds"

Fig.4

Effects of salt stress on the CAT activity of A. membranaceus seeds"

Fig.5

Effects of salt stress on the MDA content of A. membranaceus seeds"

Fig.6

Effects of salt stress on soluble protein content of A. membranaceus seeds"

Fig.7

Effects of salt stress on relative conductivity of A. membranaceus seeds"

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