Crops ›› 2020, Vol. 36 ›› Issue (3): 47-52.doi: 10.16035/j.issn.1001-7283.2020.03.008

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Drought-Resistance Identification and Evaluation of 152 Sunflower Recombinant Inbred Lines (RILs) at Seedling Stage

Zhou Zichao, Hou Jianhua(), Zhen Zilong, Shi Huimin   

  1. College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010010, Inner Mongolia, China
  • Received:2020-01-17 Revised:2020-04-08 Online:2020-06-15 Published:2020-06-10
  • Contact: Jianhua Hou E-mail:houjh68@163.com

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Abstract:

In this experiment, 152 oil sunflower recombination inbred lines were used as test materials in greenhouse pot experiment, to study the effects of drought stress on their seedling phenotype, physiological and biochemical characteristics. The drought-resistance coefficient, comprehensive drought resistance (D-value), correlation analysis, linear regression analysis and cluster analysis were used for the calculation and analysis of data to select the simple and effective identification indexes and drought resistance germplasms of sunflower at seedling stage. The results showed that the drought-resistance coefficient from high to low is in order of root-shoot ratio, root surface area, root length, root volume, electrical conductivity, SPAD value, plant height, relative water content of leaves and leaf area. Linear regression analysis and correlation analysis of drought resistance D-value showed that the nine indicators were significantly correlated with drought-resistance coefficients at seedling stage out of which the root length and root surface area are more significantly related to drought-resistance coefficients. D-value clustering grouped the tested inbred line materials into 4 categories, including 2 strong drought-tolerant types, 2 drought tolerant types, 83 weak drought-tolerant types and 65 non-drought-tolerant types. Among them, the D-values of the comprehensive drought-resistant ability of strain 149 and strain 10 were significantly higher than that of other strains.

Key words: Sunflower, Seedling stage, Recombinant inbred line, Drought resistance

Table 1

Soil water content and watering amount"

胁迫天数
Days of
stressing (d)		 土壤含水量
Soil moisture content (%)		 浇水量
Watering amount (mL)
对照
Control		 干旱胁迫
Drought stress		 对照
Control		 干旱胁迫
Drought stress
3 18.5 8.5 120 60
6 18.0 7.5 160 120
9 17.0 8.0 200 160
12 16.5 7.8 320 200
15 19.0 8.8 400 320

Table 2

Descriptive statistics of drought-resistance indicators at seedling stage"

指标
Indicator		 均值Mean 变异幅度Variability 变异系数
Coefficient of variation (%)		 广义遗传力
Generalized heritability (%)		 抗旱系数
Drought-resistance coefficient (%)
对照
Control		 干旱胁迫
Drought stress		 对照
Control		 干旱胁迫
Drought stress		 对照
Control		 干旱胁迫
Drought stress
电导率Electrical conductivity (μS/cm) 0.66 0.81 0.69 0.70 20.68 14.34 90.93 122.7
根表面积Root surface area (cm2) 3.27 5.00 7.84 10.47 36.66 32.05 61.73 152.9
根冠比Root-shoot ratio 0.09 0.24 0.33 0.61 59.60 42.58 15.52 266.7
根长Root length (cm) 21.12 27.66 53.84 56.97 35.86 39.51 46.09 131.0
根体积Root volume (cm3) 0.12 0.15 0.75 0.38 61.66 38.81 80.00 125.0
SPAD值SPAD value 33.34 30.25 32.07 27.10 15.90 12.32 79.61 90.7
叶面积Leaf area (cm2) 13.15 4.39 6.87 7.85 40.81 30.37 53.18 33.4
叶片相对含水量
Relative water content of leaves (%)		 0.87 0.60 0.30 0.55 7.70 17.01 30.77 68.9
株高Plant height (cm) 16.95 14.32 23.17 31.67 18.60 26.43 83.73 84.3

Table 3

Correlation analysis of drought-resistance coefficients of all indicators at seedling stage"

指标
Indicator		 电导率
Electrical
conductivity		 根表面积
Root surface
area		 根冠比
Root-shoot
ratio		 根长
Root
length		 根体积
Root
volume		 SPAD值
SPAD
value		 叶面积
Leaf
area		 叶片相对含水量
Relative water
content of leaves
根表面积Root surface area -0.258**
根冠比Root-shoot ratio -0.180* -0.198*
根长Root length -0.197* -0.185* -0.235**
根体积Root volume -0.186* -0.194* -0.120 0.239**
SPAD值SPAD value -0.245** -0.323** -0.272** 0.268** -0.072
叶面积Leaf area -0.180* -0.178* -0.230** 0.008 -0.419** -0.295**
叶片相对含水量Relative water content of leaves -0.237** -0.240** -0.323** 0.291** -0.376** -0.343** 0.244**
株高Plant height -0.038 -0.249** -0.159* 0.258** -0.320** -0.283** 0.296** -0.048

Table 4

The linear regression models with drought-resistance coefficient as dependent variable and the comprehensive drought-resistance subordinate function value as predictive variable"

回归变量Regression variable 回归模型Regression model R2
电导率Electrical conductivity y=0.3771x-0.0220 0.9993
根表面积Root surface area y=0.2708x-0.2707 0.9995
根冠比Root-shoot ratio y=0.0655x-0.0037 0.9995
根长Root length y=0.3795x-0.2501 0.9996
根体积Root volume y=0.1800x-0.0176 0.9989
SPAD值SPAD value y=0.9363x-0.2073 0.9991
叶面积Leaf area y=0.8528x-0.0510 0.9991
叶片相对含水量
Relative water content of leaves		 y=1.2755x-0.5599 0.9992
株高Plant height y=0.4151x-0.2117 0.9987

Table 5

Drought-resistance comprehensive evaluation of 152 sunflower materials"

样品编号
Sample number		 综合抗旱能力D值
D value of drought-resistance
comprehensive evaluation		 D值排序
D-value
order
10 0.48 1
149 0.44 2
75、138 0.39 3
120 0.35 4
126、95、110、123 0.33 5
16、77、97、109 0.32 6
6、100、143 0.31 7
1、4、5、59、116、129、151 0.30 8
48、72、85、92、96、107、146 0.29 9
7、8、17、23、38、46、49、68、73、76、102、104、119、135 0.28 10
3、9、18、24、29、37、44、51、54、56、64、69、82、114、130、133、136、145 0.27 11
2、15、22、25、27、45、61、67、106、111、124、131 0.26 12
33、50、62、70、99、103、105、113、117、122、128、139、140 0.25 13
39、42、66、71、74、84、86、94、112、115、132、150 0.24 14
19、28、31、35、47、52、53、55、58、78、79、89、93、101、108、125、127、147 0.23 15
11、12、32、137、141、142、144 0.22 16
34、40、57、63、65、87、90、91、98、118、121、134 0.21 17
14、21、30、36、43、80、88、152 0.20 18
13、26、41、60、83 0.19 19
81、148 0.18 20
20 0.17 21

Fig.1

Cluster analysis 152 sunflower inbred lines based on D-value of drought-resistance comprehensive evaluation"

Table 6

Correlation analysis of drought-resistance coefficients and D-value of drought-resistance comprehensive evaluation of 9 indicators"

株高
Plant height		 叶片相对含水量
Relative water content of leaves		 叶面积
Leaf area		 根冠比
Root-shoot ratio		 电导率
Electrical conductivity		 根长
Root length		 根表面积
Root surface area		 根体积
Root volume		 SPAD值
SPAD value
0.256** 0.374** 0.462** 0.247** 0.183* 0.578** 0.403** 0.429** 0.169*
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