Crops ›› 2018, Vol. 34 ›› Issue (4): 167-174.doi: 10.16035/j.issn.1001-7283.2018.04.028

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Effects of Salt Stress on Seed Germination and Physiological Characteristics of Apocynum venetum

Fang Jingwen,Wu Yan,Liu Zhihua   

  1. College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010019, Inner Mongolia, China
  • Received:2017-12-11 Revised:2018-03-07 Online:2018-08-20 Published:2018-08-23

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Abstract:

The effects of different salt levels [0 (CK), 100, 200, 300 and 400mmol/L NaCl] on physiological characteristics of seed germination of Apocynum venetum were investigated, taking Apocynum venetum seed from Bameng, Baotou, Tongliao, regions, in Inner Mongolia as test material. Salt resistance was comprehensively evaluated with salt damage index, salt damage level and subordinative function at this stage to investigate the response of varieties to salt stress. The results showed: The seed germination rate, germination index and vigor index were all significantly reduced when the salt concentration was in 0-400mmol/L NaCl; the content of malondialdehyde (MDA)in Bameng, Baotou and Tongliao areas showed a upward trend with the increase of salt concentration. However, the contents of supemxide dismutase (SOD), peroxidase (POD), proline (Pro) and soluble sugar of all three regions showed a upward trend at first and downward trend later with the increase of salt concentration evenly. At the same time, NaCl concentration less than 200mmol/L had a little influence on seed germination of Apocynum venetum. Salt resistance of Apocynum venetum seeds from 3 areas were evaluated by salt damage index, salt damage level and adapting subordinate function method in Fuzzy Mathematics. Ability of salt-resistance was: Bameng Apocynum venetum > Baotou Apocynum venetum > Tongliao Apocynum venetum.

Key words: Salt stress, Apocynum venetum, Physiological index, Subordinate function

Table 1

The location of study area and ecological factor"

地区
Area		 经度、纬度
Longitude and latitude		 平均温度(℃)
Average temperature		 降雨量(mm)
Rainfall		 气候类型
Climate type		 土壤特点
Soil characteristic
内蒙古农业大学农场
Inner Mongolia Agricultural
University farm		 E 111°42′
N 45°5′
 6.6

 400

 典型大陆性气候

 土壤为沙壤质暗栗钙土,pH 7.8~8.2,肥力适中,具有良好灌溉条件
巴盟Bameng
 E 106°9′
N 40°9′		 7.6
 147
 暖温带大陆性气候
 草甸土为主,表土层最高含盐量可达3.78%,盐碱化、沙化严重
包头Baotou
 E 109°52′
N 41°20′		 6.4
 310
 半干旱中温带
大陆性季风气候		 栗钙土、草甸土等易形成深厚的钙积层,工业污染重导致土壤盐碱化
通辽Tongliao
 E 119°14′
N 43°50′		 5.8
 400
 温带半干旱
大陆性气候		 土壤质地为壤质土、粉沙土,二者毛管性能强,使土壤积累盐分快而多

Table 2

Effects of salt stress on germination rate of Apocynum venetum seeds from different areas %"

NaCl浓度(mmol/L)
NaCl concentration		 包头
Baotou		 通辽
Tongliao		 巴盟
Bameng
CK 99.33±1.15a 98.67±1.15a 98.00±3.46a
100 99.33±1.15a 98.67±2.31a 97.33±1.15ab
200 97.33±1.15a 96.67±4.16a 96.67±1.15ab
300 92.00±2.00b 85.33±1.15b 92.67±3.06b
400 42.67±1.15c 37.33±2.31c 48.67±3.06c

Table 3

Effects of salt stress on germination index and vigor index of Apocynum venetum seeds from different areas"

NaCl浓度
(mmol/L)
NaCl concentration		 包头Baotou 通辽Tongliao 巴盟Bameng
发芽指数
Germination index		 活力指数
Vigor index		 发芽指数
Germination index		 活力指数
Vigor index		 发芽指数
Germination index		 活力指数
Vigor index
CK 29.06±0.63a 27.59±0.60a 26.89±0.59a 28.38±0.62a 26.48±0.77a 20.89±0.61a
100 23.22±0.48b 16.88±0.35b 23.44±1.13b 19.79±0.95b 23.11±0.38b 14.31±0.24b
200 20.37±0.38c 13.35±0.25c 19.76±1.12c 12.76±0.72c 22.50±0.93b 14.31±0.59b
300 13.96±0.27d 3.31±0.07d 11.98±0.48d 2.90±0.12d 13.00±0.86c 3.51±0.22c
400 4.33±0.30e 0.34±0.02e 3.97±0.28e 0.30±0.02e 5.30±0.35d 0.22±0.01d

Fig.1

Effects of salt stress on antioxidant enzyme activities of Apocynum venetum seeds from different areas"

Fig.3

Effects of salt stress on proline content and soluble sugar content of Apocynum venetum seeds from different areas"

Fig.2

Effects of salt stress on MDA content of Apocynum venetum seeds from different areas"

Table 4

Salt damage index and salt damage level of Apocynum venetum seeds from different areas"

NaCl浓度
(mmol/L)
NaCl concentration		 盐害指数(%)
Salt damage index		 盐害级别
Salt damage level
包头
Baotou		 通辽
Tongliao		 巴盟Bameng 包头
Baotou		 通辽Tongliao 巴盟Bameng
100 0.67 1.33 1.33 1级 1级 1级
200 2.00 2.67 2.00 1级 1级 1级
300 7.37 13.51 6.00 1级 1级 1级
400 57.03 62.15 50.35 3级 4级 3级

Table 5

Analysis of subordinate function values of salt tolerance indexes of the seeds of Apocynum venetum from different areas"

地区
Area		 发芽率
Germination rate		 发芽指数
Germination
index		 活力指数
Vigor
index		 SOD活性
SOD
activity		 MDA含量
MDA
content		 POD活性
POD
activity		 脯氨酸含量
Proline
content		 可溶性糖含量
Soluble sugar
content		 平均值Average value 排序
Order
巴盟Bameng 0.770 0.60 0.503 0.495 0.632 0.496 0.420 0.481 0.550 1
包头Baotou 0.767 0.56 0.440 0.488 0.656 0.479 0.360 0.349 0.512 2
通辽Tongliao 0.750 0.58 0.446 0.432 0.534 0.433 0.366 0.398 0.492 3

Table 6

Correlation analysis among parameters and salt concentration"

发芽率
Germination rate		 发芽指数
Germination index		 活力指数
Vigor index		 SOD 活性
SOD activity		 POD活性
POD activity		 MDA含量
MDA content		 脯氨酸含量
Proline content		 可溶性糖
Soluble sugar content
盐浓度
Salt concentration		 -0.790 -0.975** -0.986** 0.693 0.898* 0.967** 0.785 0.838
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