Crops ›› 2020, Vol. 36 ›› Issue (2): 147-155.doi: 10.16035/j.issn.1001-7283.2020.02.022

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Network Analysis of Physiological and Biochemical Indexes of Salt Tolerance in Oats

Lai Dili1,2,Fan Yu1,2,Zhu Honglin2,He Feng2,Liang Yong2,Xu Xinran2,Wen Jie2,Wang Junzhen3,Yan Jun2,Cheng Jianping1()   

  1. 1 Institute of Triticeae Crops, Guizhou University, Guiyang 550025,Guizhou, China
    2 School of Pharmacy and Bioengineering, Chengdu University/Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural Affairs, Chengdu 610106, Sichuan, China
    3 Xichang Institute of Agricultural Sciences, Liangshan 615000, Sichuan, China
  • Received:2019-09-27 Revised:2019-11-23 Online:2020-04-15 Published:2020-04-13
  • Contact: Jianping Cheng E-mail:chengjianping63@qq.com

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Abstract:

In order to explore the relationship between salt tolerance and physiological, biochemical indexes of oat seedlings under salt stress, six oat varieties with salt stress (0, 1, 2, 4, 6, 8g/kg NaCl) were treated by sand culture. The growth parameters and physiological, biochemical indicators of oat seedlings were measured on 7th, 14th and 21th day. The data were analyzed by single factor variance, correlation network and membership function. The results indicated that there were significant positive correlation among salt tolerance indexes. There were significant positive correlation between peroxidase activity, chlorophyll content and salt tolerance index. There were significant negative correlation between malondialdehyde content, soluble sugar content, proline content and salt tolerance index. There were significant negative correlation between salt tolerance index, peroxidase, chlorophyll content and 1000-seed weight.

Key words: Oat seedling, Salt tolerance index, Physiological and biochemical index, Correlation

Table 1

Basic situation of oat materials"

品种
Variety		 皮/裸燕麦
Skin/Naked oat		 千粒重(g)
1000-grain weight		 来源
Source
TO31 皮燕麦 25.6661 美国
TO48 皮燕麦 27.4195 美国
TO53 皮燕麦 23.4555 美国
BY6 裸燕麦 22.0431 中国
BY9 裸燕麦 16.7430 中国
JZY4 裸燕麦 19.7903 中国

Fig.1

Relative growth indexes of seedlings of six oat varieties under different salt concentrations and growth time"

Table 2

Effects of varieties, growth time and salt stress levels on salt tolerance indexes of oat seedlings"

耐盐指标
Salt tolerance index		 变异来源
Source of variation		 自由度
Degree of freedom		 均方值
Mean-square value		 F值
F value		 P值
P value
相对存活率 品种 5 1.195 413.521 0.001
Relative survival rate 生长时间 2 0.102 35.406 0.001
盐浓度 4 4.479 1 549.432 0.001
品种×生长时间 10 0.098 33.860 0.001
品种×盐浓度 20 0.124 43.006 0.001
生长时间×盐浓度 8 0.050 17.392 0.001
品种×生长时间×盐浓度 40 0.022 7.429 0.001
相对根数 品种 5 0.168 35.195 0.001
Relative root number 生长时间 2 1.829 383.281 0.001
盐浓度 4 0.506 106.012 0.001
品种×生长时间 10 0.218 45.622 0.001
品种×盐浓度 20 0.062 12.935 0.001
生长时间×盐浓度 8 0.186 38.880 0.001
品种×生长时间×盐浓度 40 0.035 7.308 0.001
相对苗高 品种 5 0.379 364.308 0.001
Relative seedling height 生长时间 2 0.269 258.865 0.001
盐浓度 4 6.169 5 931.928 0.001
品种×生长时间 10 0.074 70.766 0.001
品种×盐浓度 20 0.093 89.486 0.001
生长时间×盐浓度 8 0.043 40.904 0.001
品种×生长时间×盐浓度 40 0.009 8.895 0.001
相对根长 品种 5 0.088 57.897 0.001
Relative root length 生长时间 2 0.006 4.057 0.019
盐浓度 4 5.684 3 727.296 0.001
品种×生长时间 10 0.025 16.090 0.001
品种×盐浓度 20 0.044 28.765 0.001
生长时间×盐浓度 8 0.083 54.368 0.001
品种×生长时间×盐浓度 40 0.007 4.828 0.001

Fig.2

Physiological and biochemical indexes of six oat varieties seedlings under different salt concentrations and growth time"

Fig.3

Correlation network among salt tolerance indexes, physiological and biochemical indexes and 1000-seed weight of oat seedlings All correlations were significant levels (P<0.05). Ellipse, octagon and diamond represent the salt tolerance index, the biochemical index and the 1000-grain weight of seeds respectively. The edges between nodes indicate positive and negative correlations by solid and dotted lines, respectively. RRN is relative root number, RSH is relative seedling height, RSR is relative survival rate, RRL is relative root length, TGW is 1000-grain weight"

Table 3

Subordinate function value of each variety"

品种
Variety		 隶属函数值Subordinate function value 平均值
Mean
相对存活率
Relative
survival rate		 相对根数
Relative
root number		 相对苗高
Relative
seedling height		 相对根长
Relative
root length		 SPAD值
SPAD
value		 POD活性
POD
activity		 Pro含量
Pro
content		 MDA含量
MDA
content		 SS含量
Soluble sugar
content
TO31 0.5324 0.5026 0.4937 0.3643 0.5855 0.3062 0.8493 0.8325 0.7343 0.5779
TO48 0.3440 0.4356 0.3463 0.2579 0.3406 0.2571 0.7767 0.7309 0.7240 0.4681
TO53 0.6535 0.4984 0.4725 0.3419 0.6506 0.4509 0.6563 0.6759 0.6555 0.5617
BY6 0.6366 0.5643 0.5165 0.2482 0.6758 0.5610 0.7057 0.7770 0.7236 0.6010
BY9 0.6756 0.5360 0.5239 0.2787 0.6232 0.3599 0.7465 0.7304 0.7620 0.5818
JZY4 0.5578 0.4660 0.4239 0.2880 0.5705 0.4959 0.8227 0.7140 0.6218 0.5512
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