Crops ›› 2020, Vol. 36 ›› Issue (4): 45-52.doi: 10.16035/j.issn.1001-7283.2020.04.007

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Study on the Evaluation of Stay-Green Traits of Wheat and Its Correlation with Yield-Related Traits under Different Water Conditions

Yang Bin1,2(), Yan Xue1(), Wen Hongwei2,3, Wang Shuguang1, Lu Lahu2, Fan Hua1, Jing Ruilian4, Sun Daizhen1()   

  1. 1College of Agronomy, Shanxi Agricultural University, Taigu 030801, Shanxi, China
    2Institute of Wheat, Shanxi Agricultural University, Linfen 041000, Shanxi, China
    3Shanxi Province Key Laboratory of Organic Dry Farming, Taiyuan 030000, Shanxi, China
    4Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2019-11-20 Revised:2019-12-03 Online:2020-08-15 Published:2020-08-11
  • Contact: Sun Daizhen E-mail:sxxmsyb83@126.com;yanxue092@163.com;sdz64@126.com

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Abstract:

To provide support for rapidly selecting stay-green lines and for accelerating drought-resistant and high photosynthetic efficiency breeding processes, phenotypic indicators suitable for evaluating stay-green traits should be found out under multiple environmental conditions. In this study, the SPAD value, green leaf area duration (GLAD) and the change of senescence parameters under different water conditions of flag leaves in different periods of grain-filling were analyzed using recombinant inbred lines (RIL) population of 306 lines (Hanxuan 10×Lumai 14), and the correlations between them and yield-related traits were studied. The results showed that the changes of SPAD value and GLAD in RIL population were dynamic and complex processes during the whole grain-filling period, while the senescence-related parameters could not reflect the dynamic process. Under the two water conditions, SPAD value at the late filling-grain stage (20, 25 and 30 days after anthesis) was significant or highly significant correlation with grain width, grain thickness, 1000-grain weight and yield per plant. Under irrigation conditions, the GLAD 10, 13, 16, 19 and 22 days after anthesis was highly significantly correlated with the yield per plant. However, the GLAD was not significantly correlated with yield per plant under drought stress. Under different environmental conditions, compared with GLAD, the SPAD value of flag leaf has a better correlation with senescence parameters and yield-related traits in the late grain-filling stage, and the measurement of the SPAD value is relatively simple, which is more suitable for selecting stay-green lines.

Key words: Wheat, Stay-green trait, Yield, Correlation analysis

Table 1

SPAD value, green leaf area duration and senescence related parameters of flag leaves during different grain filling stages under two water conditions"

性状
Trait		 亲本Parent t值
t value		 RIL群体RIL population
旱选10号
Hanxuan 10		 鲁麦14
Lumai 14		 平均值
Mean		 标准差
Standard
deviation		 偏度
Skewness		 峰度
Kurtosis		 最小值
Minimum		 最大值
Maximum		 变异系数
Coefficient of
variation (%)
SPAD-S1 56.97/56.37 61.51/59.67 4.39*/5.99* 57.26/56.39 2.44/2.33 -0.12/-0.05 -0.08/-0.26 49.42/50.16 64.34/62.66 4.27/4.13
SPAD-S2 55.38/54.18 59.93/56.87 5.75*/3.23ns 56.24/55.44 2.53/2.56 0.02/-0.22 -0.12/0.16 48.82/45.97 64.12/61.16 4.42/4.62
SPAD-S3 53.88/51.97 59.34/52.06 9.09*/0.12ns 55.10/52.71 3.22/3.27 -0.78/-0.52 2.11/0.68 35.90/39.58 61.84/59.92 5.85/6.21
SPAD-S4 52.74/49.34 55.79/42.46 5.73*/20.05** 50.97/46.88 6.44/8.44 -1.19/-1.61 2.71/2.97 1.48/3.58 60.55/57.92 12.64/18.01
SPAD-S5 42.98/25.95 41.43/10.12 0.35ns/7.56* 34.61/18.94 9.30/12.30 -0.65/0.35 -0.03/-0.65 0.00/0.00 55.82/49.32 35.53/64.95
SPAD-S6 15.97/3.25 12.34/1.74 1.72ns/8.13* 12.96/3.97 2.12/2.87 0.85/0.70 1.81/1.20 0.00/0.00 42.55/20.38 43.58/76.82
SPAD-S7 3.71/0.00 2.11/0.00 4.82*/- 2.04/- 0.12/- 1.20/- 1.75/- 0.00/0.00 6.22/0.00 95.95/-
GLAD-D1 8.15/8.13 8.73/7.75 5.00*/3.99ns 8.53/8.32 0.34/0.43 -0.79/-0.93 0.88/1.40 7.00/6.67 9.00/9.00 3.98/5.17
GLAD-D2 8.00/7.92 8.60/6.58 5.20*/6.70* 8.30/8.03 0.49/0.59 -1.10/-1.83 1.13/1.90 4.25/4.10 9.00/9.00 5.87/7.33
GLAD-D3 7.93/6.65 7.93/5.14 1.43ns/5.60* 7.86/7.12 0.84/1.35 -0.65/-0.81 0.55/0.59 2.37/2.40 9.00/8.67 10.68/18.88
GLAD-D4 7.40/5.00 6.53/2.40 6.21*/9.37* 6.93/5.29 1.29/1.89 -1.63/-0.93 1.72/1.37 1.05/0.22 8.80/8.00 18.67/35.75
GLAD-D5 4.50/0.62 3.53/0.00 3.35ns/- 3.93/1.55 1.03/1.60 -0.15/1.19 -0.84/1.12 0.00/0.00 8.00/7.40 51.57/43.09
GLAD-D6 1.32/0.00 0.40/0.00 6.72*/- 1.02/0.22 1.35/0.64 1.73/1.80 2.85/1.88 0.00/0.00 6.25/4.20 62.52/85.77
GLAD-D7 0.30/0.00 0.00/0.00 -/- 0.10/0.03 0.35/0.19 4.34/1.47 2.01/1.62 0.00/0.00 2.75/2.40 77.30/87.16
MRS (%/d) 15.13/13.97 13.21/15.86 5.39*/9.97** 16.15/16.34 4.60/5.22 -0.26/-0.30 -0.40/-0.35 6.30/5.57 37.10/33.63 28.70/31.94
TMRS (d) 22.86/20.12 22.20/18.86 0.53ns/5.32* 22.69/20.65 1.67/1.67 -0.25/-0.63 1.10/2.94 16.14/13.26 26.73/25.85 7.37/8.10
PGMS (%) 29.80/29.61 32.91/30.19 5.15*/0.93ns 31.33/31.08 1.63/2.30 2.41/1.64 1.95/0.87 26.87/25.50 45.52/42.53 5.21/7.41
75%G 20.88/17.75 19.45/16.20 1.63ns/2.45ns 20.04/17.96 1.81/2.08 -1.13/-1.68 0.89/0.92 8.85/5.80 24.67/23.21 9.04/11.60
50%G 22.28/19.52 21.91/17.84 0.84ns/5.17* 21.91/19.85 1.66/1.72 -0.43/-1.04 0.91/1.07 15.19/11.06 26.70/25.07 7.56/8.68
25%G 23.60/20.92 23.48/19.14 0.33ns/4.76* 23.39/21.35 1.73/1.69 -0.13/-0.24 0.68/1.26 16.92/14.68 28.46/27.05 7.38/7.90
Ts (d) 18.15/14.95 12.71/12.97 4.96*/6.25* 16.37/14.23 2.65/3.31 -2.10/-1.98 1.08/1.78 8.26/2.03 22.11/19.86 16.23/19.86
To (d) 29.60/24.47 25.22/20.91 4.80*/9.65* 25.03/23.02 1.98/1.92 0.05/0.53 0.19/2.43 18.75/16.17 30.80/29.38 7.92/9.35

Table 2

Distribution characteristics of yield-related traits under different two water conditions"

性状
Trait		 亲本Parent t值
t value		 RIL群体RIL population
旱选10号
Hanxuan 10		 鲁麦14
Lumai 14		 平均值
Mean		 标准差
Standard
deviation		 偏度
Skewness		 峰度
Kurtosis		 最小值
Minimum		 最大值Maximum 变异系数Coefficient of
variation (%)
千粒重
1000-grain weight (g)		 38.54/37.52 39.12/34.02 2.83ns/5.39* 39.33/37.36 4.51/4.13 -0.49/0.17 1.31/0.03 26.28/25.22 48.80/50.40 11.47/11.57
单株产量
Yield per plant (g)		 5.20/3.99 4.93/1.92 3.59ns/6.45* 4.31/2.94 1.58/0.97 0.49/0.54 0.01/0.02 1.24/0.72 9.11/5.59 36.78/33.13
单株穗数
Spike number per plant		 4.16/3.13 3.24/1.94 2.60ns/4.78* 3.36/2.63 0.96/0.77 0.60/1.23 0.91/0.46 1.50/1.35 7.25/6.98 40.62/47.23
穗粒数
Grain number per spike		 43.40/41.24 33.02/32.99 5.75*/5.10* 40.33/36.35 6.22/6.08 0.17/-0.06 0.32/0.29 24.39/16.67 56.60/51.47 15.43/16.71
粒长Grain length (mm) 6.55/6.29 6.68/6.26 0.52ns/1.88ns 6.74/6.59 0.32/0.32 0.00/-0.03 0.00/0.13 5.89/5.59 7.73/7.70 4.76/4.89
粒宽Grain width (mm) 3.48/3.35 3.07/3.03 7.35*/6.36* 3.30/3.19 0.18/0.18 0.50/-0.54 0.97/2.22 2.76/2.24 4.01/3.74 5.53/5.61
粒厚
Grain thickness (mm)		 3.14/2.95 3.03/2.91 4.35*/4.84* 3.11/2.99 0.19/0.20 0.34/0.03 0.00/0.00 2.65/2.48 3.69/3.57 5.99/6.58

Table 3

Correlation coefficients of the SPAD value and yield related traits under two water conditions"

性状Trait SPAD-S1 SPAD-S2 SPAD-S3 SPAD-S4 SPAD-S5 SPAD-S6 SPAD-S7
粒长Grain length -0.06/0.01 -0.07/0.03 -0.01/0.04 -0.07/0.07 0.09/0.04 -0.02/-0.02 -0.06/0.04
粒宽Grain width -0.04/0.09 -0.03/0.07 -0.03/0.07 -0.09/0.05 0.21**/0.20** -0.26**/0.13* -0.13*/0.22**
粒厚Grain thickness -0.02/0.04 -0.05/0.02 -0.03/0.02 -0.04/-0.05 0.06/0.19** -0.24**/0.18** -0.10/0.21**
千粒重
1000-grain weight		 -0.04/-0.07 -0.03/-0.05 -0.05/0.09 -0.12*/0.07 0.15**/0.22** -0.12*/0.18** -0.17**/0.19**
单株产量
Yield per plant		 -0.05/0.11 -0.08/0.15* -0.17**/0.15* -0.27**/0.15** 0.27**/0.17** -0.23**/0.13* -0.20**/0.15**
单株穗数
Spike number per plant		 -0.04/0.11 -0.06/0.12* -0.13*/0.12* -0.19**/0.08 0.18**/0.01 -0.07/-0.10 -0.02/-0.06
穗粒数
Grain number per spike		 -0.14*/0.19** -0.16**/0.23** -0.22**/0.20** -0.27**/0.21** 0.26**/0.17** -0.04/0.10 -0.03/0.07

Table 4

Correlation coefficients of the green leaf area duration and yield traits under two water conditions"

性状Trait GLAD-D1 GLAD-D2 GLAD-D3 GLAD-D4 GLAD-D5 GLAD-D6 GLAD-D7
粒长Grain length -0.16**/0.17** 0.14*/0.12* 0.12*/0.07 0.11*/0.03 0.05/0.03 -0.01/0.03 -0.09/0.02
粒宽Grain width -0.03/0.05 0.05/-0.01 0.05/0.03 0.12*/0.13* 0.30**/0.18** -0.29**/0.14* -0.16**/0.04
粒厚Grain thickness -0.05/0.07 0.01/-0.01 0.03/0.06 0.05/0.04 0.12*/0.15* -0.23**/0.19** -0.16**/0.08
千粒重
1000-grain weight		 -0.07/0.21** 0.07/0.16** 0.16**/0.15** 0.17**/0.13* 0.20**/0.14* -0.11/0.08 -0.05/0.05
单株产量
Yield per plant		 -0.15**/0.11 0.16**/0.09 0.22**/0.04 0.20**/0.06 0.19**/0.07 -0.09/0.07 -0.04/0.10
单株穗数
Spike number per plant		 -0.11*/0.04 0.10/0.02 0.15*/-0.02 0.12*/-0.02 0.09/-0.06 -0.08/-0.12* -0.08/-0.14*
穗粒数
Grain number per spike		 -0.18**/0.08 0.23**/0.10 0.23**/0.04 0.19**/0.07 0.16**/0.10 -0.02/0.14* -0.06/0.02

Table 5

Correlation coefficients of the senescence related parameters and yield traits under two water conditions"

性状Trait MRS TMRS PGMS Ts To 75%G 25%G 50%G
粒长Grain length -0.09/-0.09 0.05/0.02 -0.05/0.08 -0.11/0.06 -0.00/-0.06 0.09/0.03 0.03/-0.03 0.06/0.00
粒宽Grain width -0.12*/0.12* 0.28**/0.15** -0.08/0.06 -0.03/-0.03 -0.30**/0.20** 0.17**/0.05 0.29**/0.17** 0.25**/0.12*
粒厚Grain thickness -0.08/0.10 0.14*/0.08 -0.13*/0.13* -0.06/-0.10 -0.19**/0.16** 0.04/-0.03 0.16**/0.11 0.11/0.04
千粒重
1000-grain weight		 -0.04/-0.07 0.19**/0.11 -0.04/0.18** -0.16**/0.01 -0.15*/0.13* 0.20**/0.06 0.18**/0.12* 0.20**/0.10
单株产量
Yield per plant		 -0.21**/-0.20** 0.15**/-0.04 -0.07/-0.02 -0.29**/0.13* -0.03/-0.13* 0.26**/0.07 0.11/-0.07 0.19**/0.00
单株穗数
Spike number per plant		 -0.19**/-0.09 0.06/-0.07 -0.08/0.01 -0.21**/0.05 -0.04/-0.11 0.16**/0.07 0.02/-0.09 0.09/-0.05
穗粒数
Grain number per spike		 -0.16**/-0.15* 0.12*/-0.01 -0.02/-0.08 -0.21**/0.13* -0.03/-0.10 0.19**/0.08 0.09/-0.04 0.14*/0.02
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