Crops ›› 2020, Vol. 36 ›› Issue (4): 84-90.doi: 10.16035/j.issn.1001-7283.2020.04.012

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Isolation and Expression Analysis of Transcription Factor Gene TaMYB70 in Wheat

Xu Yuanyuan1(), Zhao Peng2, Hong Quanchun1, Zhu Xiaoqin1, Pei Dongli1()   

  1. 1School of Biology and Food Sciences, Shangqiu Normal University/Henan Provincial Key University Laboratory of Plant-Microbe Interactions, Shangqiu 476000, Henan, China
    2School of Humanities, Shangqiu Normal University, Shangqiu 476000, Henan, China
  • Received:2019-12-16 Revised:2020-03-03 Online:2020-08-15 Published:2020-08-11
  • Contact: Pei Dongli E-mail:xuyy1108@163.com;peidongli@126.com

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Abstract:

To excavate the function of MYB transcription factor TaMYB70 in wheat, TaMYB70 gene was isolated using homology cloning strategy. The expression patterns of TaMYB70 under different stresses were examined by quantitative real-time PCR (qRT-PCR). The results showed that the TaMYB70 partial cDNA was 1 272bp and contained an open reading frame of 1 108bp, encoding 335 amino acids. TaMYB70 protein contained two Myb-type HTH DNA binding domains which were helix-turn-helix motifs. The homologous sequence alignment indicated that the TaMYB70 had higher homology with MYB44 from other plants, such as Aegilops tauschii and Brachypodium distachyum (L.). TaMYB70 belongs to the same branch as the 22nd members of the arabidopsis R2R3-MYB transcription factors. The expression of TaMYB70 was upregulated under ABA treatment, and downregulated under PEG and NaCl stresses, and which may be involved in stress response of wheat.

Key words: Wheat, TaMYB70, Transcription factor, R2R3-MYB, Expression analysis

Table 1

The primers used in this study for qRT-PCR"

引物名
Primer name		 引物序列(5′-3′)
Primer sequence (5′-3′)		 产物长度
Length of PCR
product (bp)
ACTIN-F TCTGTCCTTGTATGCCAGCG 195
ACTIN-R GCGGTTGTTGTGAGGGAGTA
MYB-YG-F GGTCCCTCATCAGCAAGTCC 213
MYB-YG-R TCCAGTGGTTCTTGATGGCG

Fig.1

The amplification results of wheat TaMYB70 gene"

Fig.2

Functional domain prediction of wheat TaMYB70 protein"

Fig.3

Three-dimensional structure model of wheat TaMYB70 protein"

Fig.4

Multiple sequence alignments of wheat TaMYB70 protein"

Fig.5

Sequence analysis of wheat TaMYB70 protein domain"

Fig.6

Phylogenetic tree of TaMYB70 and the MYB transcription factors in Arabidopsis"

Fig.7

Expression characteristics of wheat TaMYB70 gene under abiotic stress"

[1] Stracke R, Werber M, Weisshaar B . The R2R3-MYB gene family in Arabidopsis thaliana. Current Opinion in Plant Biology, 2001,4(5):447-456.
[2] 陈俊, 王宗阳 . 植物MYB类转录因子研究进展. 植物生理学与分子生物学学报, 2002,28(2):81-88.
[3] Paz-Ares J, Ghosal D, Wienand U , et al. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators. The EMBO Journal, 1987,6(12):3553-3558.
[4] Feller A, Machemer K, Braun E L , et al. Evolutionary and comparative analysis of MYB and bHLH plant transcription factors. The Plant Journal, 2011,66(1):94-116.
[5] Dubos C, Stracke R, Grotewold E , et al. MYB transcription factors in Arabidopsis. Trends in Plant Science, 2010,15(10):573-581.
[6] 樊锦涛, 蒋琛茜, 邢继红 , 等. 拟南芥R2R3-MYB家族第22亚族的结构与功能. 遗传, 2014,36(10):985-994.
[7] Cheong Y H, Chang H S, Gupta R , et al. Transcriptional profiling reveals novel interactions between wounding,pathogen,abiotic stress,and hormonal responses in Arabidopsis. Plant Physiology, 2002,129(2):661-677.
[8] Fowler S, Thomashow M F . Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. The Plant Cell, 2002,14(8):1675-1690.
[9] Zhao Y, Xing L, Wang X G , et al. The ABA receptor PYL8 promotes lateral root growth by enhancing MYB77-dependent transcription of auxin-responsive genes. Science Signaling, 2014,7(328):ra53.
[10] Jung C K, Seo J S, Han S W , et al. Overexpression of AtMYB44 enhances stomatal closure to confer abiotic ctress tolerance in transgenic Arabidopsis. Plant Physiology, 2008,146(2):623-635.
[11] Li D K, Li Y, Zhang L , et al. Arabidopsis ABA receptor RCAR1/PYL9 interacts with an R2R3-Type MYB transcription factor,AtMYB44. International Journal of Molecular Sciences, 2014,15(5):8473-8490.
[12] Persak H, Pitzschke A . Dominant repression by Arabidopsis transcription factor MYB44 causes oxidative damage and hypersensitivity to abiotic stress. International Journal of Molecular Sciences, 2014,15(2):2517-2537.
[13] Shamloo-Dashtpagerdi R, Razi H, Ebrahimie E , et al. Molecular characterization of Brassica napus stress related transcription factors,BnMYB44 and BnVIP1,selected based on comparative analysis of Arabidopsis thaliana and Eutrema salsugineum transcriptomes. Molecular Biology Reports, 2018,45(5):1111-1124.
[14] Liu C Y, Xie T, Chen C J , et al. Genome-wide organization and expression profiling of the R2R3-MYB transcription factor family in pineapple (Ananas comosus). BMC Genomics, 2017,18(1):503-519.
[15] Yang Y, Zhang L B, Chen P , et al. UV-B photoreceptor UVR8 interacts with MYB73/MYB77 to regulate auxin responses and lateral root development. The EMBO Journal, 2019,39(2):1-15.
[16] He J, Liu Y Q, Yuan D Y , et al. An R2R3 MYB transcription factor confers brown planthopper resistance by regulating the phenylalanine ammonia-lyase pathway in rice. Proceedings of the National Academy of Sciences of the United States of America, 2020,117(1):271-277.
[17] 赵哲, 李德款, 袁德志 , 等. AtMYB44与ABI1竞争性结合ABA受体RCAR1的研究. 四川大学学报(自然科学版), 2015,52(3):663-667.
[18] 樊锦涛 . 拟南芥AtMYB73响应干旱机制初探. 保定:河北农业大学, 2015.
[19] Kim J H, Nguyen N H, Jeong C Y , et al. Loss of the R2R3 MYB,AtMyb73,causes hyper-induction of the SOS1 and SOS3 genes in response to high salinity in Arabidopsis. Journal of Plant Physiology, 2013,170(16):1461-1465.
[20] 庞茜, 赵亚婷, 樊锦涛 , 等. AtMYB73基因正调控拟南芥对盐胁迫的响应. 河北农业大学学报, 2017,40(5):48-47,59.
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