河北省小麦品质相关基因的KASP标记检测

doi:10.16035/j.issn.1001-7283.2020.04.009

Abstract

Abstract:

To accelerate wheat quality improvement, 153 wheat varieties of Hebei Province since 1970s were analyzed using the high-throughput KASP markers. These functional markers included the detection of 1RS/1BL and 1RS/1AL translocation lines, high-molecular-weight glutenin subunits, grain hardness, gene related to grain protein content and amylose synthesis, and grain color. The results showed that the translocation lines of 1RS/1BL and 1RS/1AL accounted for 43.14% and 9.15% respectively. Glu-Ax1 and Ax2* subunits accounted for 39.22% and 11.76% respectively at Glu-A1 locus; Bx7OE subunits accounted for 1.96% at Glu-B1 locus, 1Dx5+1Dy10 subunits accounted for 13.07% at Glu-D1 locus. Three markers were used to detect the hardness genes of wheat grain, and allelic variations Pina-D1b, Pinb-D1b and Pinb-B2b accounted for 5.88%, 70.59%, and 35.95% respectively. No favorable allelic variations were detected in Gpc-B1 and Wx-B1, which were related to increasing grain protein content and amylose synthesis, respectively. Ten genes, Ppo-A1, Ppo-D1, Psy-A1, Psy-B1, Psy-D1/Sr25, Zds-A1, Lox-B1, TaLyc-B1, TaPds-B1 and TaPod-A1 related to grain quality color were also detected and the proportion of favourable alleles were 69.93%, 7.19%, 27.45%, 9.15%, 100.00%, 15.03%, 75.82%, 71.90%, 25.49% and 25.49%, respectively. The favorable allele variation of Ppo-D1 locus showed a decreasing trend year by year. KASP markers can detect favorable allele of wheat quality-related genes effectively, which has a good application prospect in wheat quality improvement in Hebei Province.

Key words: Wheat, KASP markers, Quality

Shan Zilong, Ban Jinfu, Zhao Yankun, Cao Qiao, Tian Guoying, He Mingqi, Gao Zhenxian. Detection of Quality-Related Genes in the Wheat Varieties Authorized in Hebei Province by KASP Markers[J].Crops, 2020, 36(4): 64-71.

Figures/Tables 4

Table 1

Names of the 153 test variety"

序号 Number	品种 Variety	序号 Number	品种 Variety	序号 Number	品种 Variety	序号 Number	品种 Variety
1	冀麦1号Jimai 1	31	津丰一号Jinfeng 1	61	石新733 Shixin 733	91	科农199 Kenong 199
2	冀麦2号Jimai 2	32	泰山1号Taishan 1	62	邯4589 Han 4589	92	金麦一号Jinmai 1
3	冀麦3号Jimai 3	33	泰山5号Taishan 5	63	冀5385 Ji 5385	93	藁优9908 Gaoyou 9908
4	冀麦4号Jimai 4	34	冀麦30号Jimai 30	64	科农9204 Kenong 9204	94	邯00-7086 Han 00-7086
5	冀麦5号Jimai 5	35	冀麦31号Jimai 31	65	石家庄9号Shijiazhuang 9	95	衡6599 Heng 6599
6	冀麦6号Jimai 6	36	冀麦33号Jimai 33	66	藁优9409 Gaoyou 9409	96	邯麦11号Hanmai 11
7	冀麦7号Jimai 7	37	冀麦36号Jimai 36	67	衡7228 Heng 7228	97	冀7369 Ji 7369
8	冀麦8号Jimai 8	38	冀麦37号Jimai 37	68	冀5579 Ji 5579	98	冀5265 Ji 5265
9	冀麦9号Jimai 9	39	冀麦38号Jimai 38	69	石家庄10号Shijiazhuang 10	99	永麦1号Yongmai 1
10	冀麦10号Jimai 10	40	冀麦42号Jimai 42	70	石新539 Shixin 539	100	金麦54 Jinmai 54
11	冀麦11号Jimai 11	41	衡4041 Heng 4041	71	晶白麦1号Jingbaimai 1	101	石优17号Shiyou 17
12	冀麦13号Jimai 13	42	河农326 Henong 326	72	白硬冬2号Baiyingdong 2	102	河农825 Henong 825
13	冀麦14号Jimai 14	43	河农972 Henong 972	73	藁优9415 Gaoyou 9415	103	石新616 Shixin 616
14	冀麦15号Jimai 15	44	25993	74	衡4338 Heng 4338	104	衡4399 Heng 4399
15	冀麦16号Jimai 16	45	石5093 Shi 5093	75	河农822 Henong 822	105	邢麦6号Xingmai 6
16	冀麦17号Jimai 17	46	中麦9号Zhongmai 9	76	衡观35 Hengguan 35	106	石麦18号Shimai 18
17	冀麦18号Jimai 18	47	邯4564 Han 4564	77	石麦12号Shimai 12	107	河农6049 Henong 6049
18	冀麦19号Jimai 19	48	河农85-9 Henong 85-9	78	石麦14号Shimai 14	108	冀6358 Ji 6358
19	冀麦20号Jimai 20	49	5099	79	衡5229 Heng 5229	109	藁优2018 Gaoyou 2018
20	冀麦21号Jimai 21	50	8901-11	80	NC 2号NC 2	110	邯麦12号Hanmai 12
21	冀麦22号Jimai 22	51	河农341 Henong 341	81	良星99 Liangxing 99	111	衡0628 Heng 0628
22	冀麦23号Jimai 23	52	邯5316 Han 5316	82	科农213 Kenong 213	112	石新811 Shixin 811
23	冀麦24号Jimai 24	53	梁麦2号Liangmai 2	83	师栾02-1 Shiluan 02-1	113	河农58-3 Henong 58-3
24	冀麦25号Jimai 25	54	沧核030 Canghe 030	84	冀丰703 Jifeng 703	114	石优20号Shiyou 20
25	冀麦26号Jimai 26	55	石4185 Shi 4185	85	石麦16号Shimai 16	115	河农9206 Henong 9206
26	冀麦27号Jimai 27	56	高优503 Gaoyou 503	86	石麦15号Shimai 15	116	衡136 Heng 136
27	冀麦28号Jimai 28	57	石家庄8号Shijiazhuang 8	87	石新828 Shixin 828	117	邯麦13 Hanmai 13
28	冀麦29号Jimai 29	58	邯优3475 Hanyou 3475	88	科农1093 Kenong 1093	118	金禾9123 Jinhe 9123
29	冀麦5418 Jimai 5418	59	衡95观26 Heng 95 guan 26	89	藁优9618 Gaoyou 9618	119	石麦19 Shimai 19
30	衡水6404 Hengshui 6404	60	邯6172 Han 6172	90	河农4198 Henong 4198	120	河农5290 Henong 5290
序号 Number	品种 Variety	序号 Number	品种 Variety	序号 Number	品种 Variety	序号 Number	品种 Variety
121	邯麦14 Hanmai 14	130	河农7069 Henong 7069	138	冀麦120 Jimai 120	146	科农2011 Kenong 2011
122	婴泊700 Yingbo 700	131	冀麦518 Jimai 518	139	中麦8号Zhongmai 8	147	邯农1412 Hannong 1412
123	衡4444 Heng 4444	132	藁优5766 Gaoyou 5766	140	衡科6021 Hengke 6021	148	石农086 Shinong 086
124	邢麦7号Xingmai 7	133	轮选103 Lunxuan 103	141	中信麦99 Zhongxinmai 99	149	石麦26-1 Shimai 26-1
125	农大399 Nongda 399	134	衡S29 Heng S29	142	邯生730 Hansheng 730	150	石麦26-2 Shimai 26-2
126	石新633 Shixin 633	135	石麦24 Shimai 24	143	石麦25 Shimai 25	151	石麦25 Shimai 25
127	石麦22 Shimai 22	136	科农2009 Kenong 2009	144	邯麦17 Hanmai 17	152	石麦28 Shimai 28
128	科农1006 Kenong 1006	137	藁优5218 Gaoyou 5218	145	邯麦15 Hanmai 15	153	石麦29 Shimai 29
129	冀麦585 Jimai 585

Table 1

Fig.1

Table 2

The distribution of KASP markers for quality-related genes in Hebei wheat varieties"

性状 Trait	优异等位变异 Favourable allele	含优异等位变异品种编号 Number of varieties with favourable allele	含杂合位点品种编号 Number of varieties with heterozygous locus
黑麦易位系 Rye translocation line	1RS/1BL	10、16~18、24~26、34、36、39、41~48、52、53、55、57、59、60、62、64、68、70、74~77、79~81、84~88、92~94、97、100、101、106、107、111、114、115、117、119、121、123、124、 127~129、134、138、140、142、145、146、152
	1RS/1AL	19、23、35、64、69、72、74、82、84、87、89、104、109、118	33、37、54、56、63、95、101、113、119、134
高分子量谷蛋白亚基 High molecular weight glutenin subunits	Glu-Ax1	2、3、5、8、9、10、12、13、18、21、22、25、27、28、30、34、36~39、42、43、48、51、57、59、62、64、69、74、76、77、84、86、87、95、97、103、106、112、117~119、121、124、 127、128、130、131、133、135、136、139、141、143、144、 147、148、151、153
	Glu-Ax2*	4、6、15、16、20、31~33、35、41、45、47、58、68、75、85、90、111
	Bx7OE	5、32、95
	1Dx5+1Dy10	36、49、50、66、73、79、83、87、89、91、98、103~105、107、 122、125、126、132、137
籽粒硬度 Kernel hardness	Pina-D1b	50、73、79、83、98、103、122、126、132	56、90、96、137
籽粒硬度 Kernel hardness	Pinb-D1b	3、4、6、8、10、11、13、15~17、19、20、22~25、28、30、31、37~39、41、44~47、49、51~53、55、57~61、63、64、66、68~ 72、74~76、78、80~82、84~89、91~95、97、99、100、102、104~ 118、120、121、123~125、127、129、133~136、138~144、146~ 153	29、48、90、101、126、128
	Pinb-B2b	1、2、4、8、11~13、18、31~33、35、37、43、44、50、53、59、66、68、69、72~74、77、79、82~84、91、93、98、102、104、107、109、116、122、124~126、128、132、133、135、136、139、141、143、144、147、148、150、151、153	38、48、112、149
色泽 Color	Ppo-A1b	2~10、12~17、19~24、26、27、31、32、34、36~38、40、43、44、46、50~53、55、56、60、62、63、66、69、70、72、73、75~80、82、83、85~87、89、91~94、96、98、100、103、104、106~111、114~118、121、122、125、127~148、150、151、153	1、11、18、25、28~30、33、35、39、41、42、45、47~49、54、57~59、61、64、65、67、68、71、74、81、84、88、90、95、97、99、101、102、105、112、113、119、120、123、124、126、149、152
	Ppo-D1a	2、3、8、12、22、26、40、54、62、77、109
	Psy-A1b	7、14、17、20、23、26、29、33、39、41、45、47、54、55、57、59、61、69、70、72、75、80、82、86、92、93、96、97、102、111、115~117、119、123、126~128、132、134、137、145、
	Psy-B1a或Psy-B1b	19、25、28、52、60、94、99、100、111、114、120、130、140、145
	TaZds-A1a	2、5、14、27、32、35、40、45、49、50、66、72、73、79、82、 83、89、90、103、106、122、132、137	13、29、58、63、67、68、96、112
	Lox-B1b	1~25、27~33、35~38、40~49、51~56、58、60、61、63~65、67~ 71、74、76、80、81、84、87、88、90~96、99~105、109~116、119、120、123、125、133~136、138~153
	TaLyc-B1a	2、4~9、11、12、14、15、18、20、23、25~27、30、31、34、36、38、40、41、43~45、47、48、50、51~57、59~61、64~ 66、69~73、79~83、86、88~90、92~96、98~103、106~111、113~118、120~123、125~130、132~148、151~153	3、29、37、39、49、58、67、76、78、112
	TaPds-B1b	19、26、31、33、35、37、39、42、51、55、64、70、75、80、81、85、86、88、92、93、97、104、106、108、115~118、123、127、129、130、135、138、143、146、151~153	29、49、54、65、119、139、149
	TaPod-A1b	1、7、8、11、12、19、26、34、35、40、49、58、62、64、67、73、83、87、91、92、99、103、104、106、110、112、120~ 122、128、132、135、137、138、143、146、147、150、151	29、32、33、44、48、53、65、79、90、95、96、109、118、125、133、136、139、141、144、148、149

Table 2

Table 3

References 40

[1]	Altenbach S, Chang H C, Yu X , et al. Elimination of omega-1,2 gliadins from bread wheat (Triticum aestivum) flour: effects on immunogenic potential and end-use quality. Frontiers in Plant Science, 2019,10:580-591.
[2]	Geng H, Shi J, Fuerst E , et al. Physical mapping of peroxidase genes and development of functional markers for TaPod-D1 on bread wheat chromosome 7D. Frontiers in Plant Science, 2019,10:523-535.
[3]	Chaudhary N, Dangi P, Khatkar B S . Relationship of molecular weight distribution profile of unreduced gluten protein extracts with quality characteristics of bread. Food Chemistry, 2016,210:325-331.
[4]	Ren T H, Chen F, Zou Y T , et al. Evolutionary trends of microsatellites during the speciation process and phylogenetic relationships within the genus Secale. Genome, 2011,54(4):316-326.
[5]	Korzun V . About the origin of 1RS.1BL wheat-rye chromosome translocations from Germany. Plant Breeding, 2010,116(6):537-540.
[6]	Lelley T, Eder C, Grausgruber H . Influence of 1BL.1RS wheat-rye chromosome translocation on genotype by environment interaction. Journal of Cereal Science, 2004,39(3):313-320.
[7]	Graybosch R A . Mini Review: Uneasy Unions: Quality effects of rye chromatin transfers to wheat. Journal of Cereal Science, 2001,33(1):3-16.
[8]	Moreno-Sevilla B, Baenziger P S, Shelton D R , et al. Agronomic performance and end-use quality of 1B vs. 1BL/1RS genotypes derived from winter wheat ‘Rawhide’. Crop Science, 1995,35(6):1607-1612.
[9]	Burnett C J, Lorenz K J, Carver B F . Effects of the 1B/1R translocation in wheat on composition and properties of grain and flour. Euphytica, 1995,86(3):159-166.
[10]	Carver B F . Comparison of related wheat stocks possesing 1B or T1BL·1RS schromosomes: grain and flour quality. Crop Science, 1995,35(5):1316-1321.
[11]	Hussain A, Lukow O M . Characterization of the 1B/1R translocation in wheat using water extractable protein concentrate. Euphytica, 1994,78(1/2):109-113.
[12]	Graybosch R A, Peterson C J, Hansen L E , et al. Comparative flour quality and protein characteristics of 1BL/1RS and 1AL/1RS wheat-rye translocation lines. Journal of Cereal Science, 1993,17(2):95-106.
[13]	Li Z, Ren T, Yan B , et al. A mutant with expression deletion of gene Sec-1 in a 1RS.1BL line and its effect on production quality of wheat. PLoS ONE, 2016,11(1):e0146943.
[14]	Payne P I, Law C N, Mudd E E . Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin,a major protein of wheat endosperm. Theoretical and Applied Genetics, 1980,58(3/4):113-120.
[15]	Payne P I, Holt L M, Krattiger A F , et al. Relationships between seed quality characteristics and HMW glutenin subunit composition determined using wheats grown in Spain. Journal of Cereal Science, 1988,7(3):229-235.
[16]	He Z, Liu L, Xia X , et al. Composition of HMW and LMW glutenin subunits and their effects on dough properties,pan bread,and noodle quality of Chinese bread wheats. Cereal Chemistry, 2005,82(4):345-350.
[17]	Payne P I, Holt L M, Law C N . Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin: Part 1: Allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theoretical and Applied Genetics, 1981,60(4):229-236.
[18]	Vázquez D, Berger A G, Cuniberti M , et al. Influence of cultivar and environment on quality of Latin American wheats. Journal of Cereal Science, 2012,56(2):196-203.
[19]	Peña E, Bernardo A, Soler C , et al. Relationship between common wheat (Triticum aestivum L.) gluten proteins and dough rheological properties. Euphytica, 2005,143(1/2):169-177.
[20]	Liu W, Zhang Y Z, Gao X , et al. Comparative proteome analysis of glutenin synthesis and accumulation in developing grains between superior and poor quality bread wheat cultivars. Journal of the Science of Food and Agriculture, 2011,92(1):106-115.
[21]	Figueroa J D C, Maucher T, Reule W , et al. Influence of high molecular weight glutenins on viscoelastic properties of intact wheat kernel and relation to functional properties of wheat dough. Cereal Chemistry, 2009,86(2):139-144.
[22]	Hernández-Estrada Z J, Rayas-Duarte P, Cárdenas J d D F . Creep recovery of wet gluten and high-molecular-weight glutenin subunit composition: relationship with viscoelasticity of dough and breadmaking quality of hard red winter wheat. Cereal Chemistry, 2017,94(2):223-229.
[23]	Wang X L, Zhang Y Q, Zhang B , et al. Comparison of quality properties between HMW-GSs 5+10 and 2+12 NILs under three common wheat genetic backgrounds. Cereal Chemistry, 2018,95(4):575-583.
[24]	Ma X L, Sajjad M, Wang J , et al. Diversity,distribution of puroindoline genes and their effect on kernel hardness in a diverse panel of Chinese wheat germplasm. BMC Plant Biology, 2017,17(1):158-169.
[25]	Bhave M, Morris C F . Molecular genetics of puroindolines and related genes: allelic diversity in wheat and other grasses. Plant Molecular Biology, 2008,66(3):205-219.
[26]	Qamar Z U, Bansal U K, Chong M D , et al. Detection of puroindoline (Pina-D1 and Pinb-D1) allelic variation in wheat landraces. Journal of Cereal Science, 2014,60(3):610-616.
[27]	Catherine R, Mireille D, Fanny L , et al. Improving the yellow pigment content of bread wheat flour by selecting;the three homoeologous copies of Psy1. Molecular Breeding, 2013,31(1):87-99.
[28]	Harjes C E, Rocheford T R, Ling B , et al. Natural genetic variation in lycopene epsilon cyclase tapped for maize biofortification. Science, 2008,319(5861):330-333.
[29]	Leenhardt F, Lyan B, Rock E , et al. Genetic variability of carotenoid concentration,and lipoxygenase and peroxidase activities among cultivated wheat species and bread wheat varieties. European Journal of Agronomy, 2006,25(2):170-176.
[30]	He X Y, He Z H, Zhang L P , et al. Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat. Theoretical and Applied Genetics, 2007,115(1):47-58.
[31]	Rasheed A, Wen W, Gao F , et al. Development and validation of KASP assays for genes underpinning key economic traits in bread wheat. Theoretical and Applied Genetics, 2016,129(10):1843-1860.
[32]	Gupta P K, Kumar J, Mir R R , et al. Marker-assisted selection as a component of conventional plant breeding. Plant Breeding Reviews, 2010(23):145-217.
[33]	Andersen J R, Lübberstedt T . Functional markers in plants. Trends in Plant Science, 2003,8(11):554-560.
[34]	Lau W C P, Rafii M Y, Ismail M R , et al. Review of functional markers for improving cooking,eating,and the nutritional qualities of rice. Frontiers in Plant Science, 2015,6:832-842.
[35]	杨子博, 顾正中, 周羊梅 , 等. 江苏淮北地区小麦品种资源籽粒硬度基因等位变异的KASP检测. 麦类作物学报, 2017,37(2):153-161.
[36]	韩小东, 张荣志, 宋国琦 , 等. Fhb1基因不同等位变异在小麦品种资源中的分布. 山东农业科学, 2018,50(8):1-6.
[37]	桑大军, 许为钢, 胡琳 , 等. 河南省小麦品种白粉病抗性基因的分子鉴定及分子标记辅助育种. 华北农学报, 2006,21(1):86-91.
[38]	张勇, 申小勇, 张文祥 , 等. 高分子量谷蛋白5+10亚基和1B/1R易位分子标记辅助选择在小麦品质育种中的应用. 作物学报, 2012,38(10):1743-1751.
[39]	程斌, 辛智海, 高旭 , 等. 贵州糯质小麦的分子标记辅助育种研究. 种子, 2017,36(12):39-43.
[40]	Bradbury L M, Fitzgerald T L, Henry R J , et al. The gene for fragrance in rice. Plant Biotechnology Journal, 2010,3(3):363-370.

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性状Trait	优异单倍型 Favourable haplotype	1970-1979		1980-1989		1990-1999		2000-2009		2010-2018
性状Trait	优异单倍型 Favourable haplotype	数目 Number	频率 Frequency (%)	数目 Number	频率 Frequency (%)	数目 Number	频率 Frequency (%)	数目 Number	频率 Frequency (%)	数目 Number	频率 Frequency (%)
黑麦易位系 Rye translocation line	1RS/1BL	0	0.00	7	29.17	14	60.87	31	50.00	14	40.00
黑麦易位系 Rye translocation line	1RS/1AL	0	0.00	2	8.33	1	4.35	11	17.74	0	0.00
高分子量谷蛋白亚基 High molecular weight glutenin subunits	Glu-Ax1	5	55.56	10	41.67	9	39.13	18	29.03	18	51.43
	Glu-Ax2*	2	22.22	6	25.00	4	17.39	6	9.68	0	0.00
	Bx7OE	1	11.11	1	4.17	0	0.00	1	1.61	0	0.00
	Bx13	0	0.00	0	0.00	0	0.00	0	0.00	0	0.00
	1Dx5+1Dy10	0	0.00	0	0.00	3	13.04	12	19.35	5	14.29
籽粒硬度 Kernel hardness	Pina-D1b	0	0.00	0	0.00	1	4.35	5	8.06	3	8.57
籽粒硬度 Kernel hardness	Pinb-D1b	4	44.44	15	62.50	13	56.52	50	80.65	26	74.29
	Pinb-B2b	4	44.44	7	29.17	6	26.09	20	32.26	18	51.43
色泽 Color	Ppo-A1b	8	88.89	17	70.83	14	60.87	40	64.52	28	80.00
色泽 Color	Ppo-D1a	3	33.33	3	12.50	2	8.70	3	4.84	0	0.00
	Psy-A1b	1	11.11	7	29.17	6	26.09	19	30.65	9	25.71
	Psy-B1a或Psy-B1b	0	0.00	3	12.50	1	4.35	6	9.68	4	11.43
	Psy-D1a	9	100.00	24	100.00	23	100.00	62	100.00	35	100.00
	TaZds-A1a	2	22.22	3	12.50	5	21.74	10	16.13	3	8.57
	Lox-B1a	9	100.00	23	95.83	20	86.96	40	64.52	24	68.57
	TaLyc-B1b	7	77.78	12	50.00	17	73.91	44	70.97	30	85.71
	TaPds-B1b	0	0.00	4	16.67	6	26.09	18	29.03	11	31.43
	TaPod-A1b	3	33.33	4	16.67	4	17.39	15	24.19	13	37.14

Detection of Quality-Related Genes in the Wheat Varieties Authorized in Hebei Province by KASP Markers

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[3]	Fan Yuanyuan, Wu Haimei, Pang Lei, Lu Jianlong, Xia Bowen, Yang Xuhai. Effects of Straw Mulching on Wheat Yield in Different Ecological Regions in Northern Semi-Arid Areas of China Based on Meta Analysis [J]. Crops, 2020, 36(4): 143-149.
[4]	Liu Dongjun, Song Weifu, Yang Xuefeng, Zhao Lijuan, Song Qingjie, Zhang Chunli, Xin Wenli, Xiao Zhimin. Progress of Wheat Fhb1 Gene Locating and Cloning and Its Utilization in the Resistance Breeding [J]. Crops, 2020, 36(4): 16-20.
[5]	Yang Yongqing, Gao Fangfang, Ma Yajun, Chen Xin, Zhang Jie. Effects of Different Fertilizer Treatments on Yield, Quality and Economic Benefit of Foxtail Millet in Dry Farming Area of Shanxi Province [J]. Crops, 2020, 36(4): 195-201.
[6]	Zhang Qian, Li Yaofa, Wang Shulin, Wang Yan, Feng Guoyi, Lin Yongzeng, Liang Qinglong, Lei Xiaopeng, Qi Hong. Effects of Strip-Planting of Cotton-Wheat on Cotton Aphid [J]. Crops, 2020, 36(4): 206-210.
[7]	Yang Ziguang, Guo Lilei, Zhang Ke, Sun Junwei, Meng Limei. Development Trend of the Major Traits of Winter Wheat Varieties (Lines) in the Huang-Huai Dryland [J]. Crops, 2020, 36(4): 30-36.
[8]	Wang Zhongqiu, Ying Pengfei, Chen Mengtao, He Qiongying, Hu Xin. Analysis of Grain and Quality Traits of Chromosome Arm Substitution Lines of Triticum dicoccoides in the Background of Triticum aestivum [J]. Crops, 2020, 36(4): 37-44.
[9]	Yang Bin, Yan Xue, Wen Hongwei, Wang Shuguang, Lu Lahu, Fan Hua, Jing Ruilian, Sun Daizhen. Study on the Evaluation of Stay-Green Traits of Wheat and Its Correlation with Yield-Related Traits under Different Water Conditions [J]. Crops, 2020, 36(4): 45-52.
[10]	Chen Weiguo, Zhang Zheng, Shi Yugang, Cao Yaping, Wang Shuguang, Li Hong, Sun Daizhen. Drought-Tolerance Evaluation of 211 Wheat Germplasm Resources [J]. Crops, 2020, 36(4): 53-.
[11]	Xu Yuanyuan, Zhao Peng, Hong Quanchun, Zhu Xiaoqin, Pei Dongli. Isolation and Expression Analysis of Transcription Factor Gene TaMYB70 in Wheat [J]. Crops, 2020, 36(4): 84-90.
[12]	Zhu Zhengbin, Yang yong, Feng Linhao, Lu Yan, Shen Xuelin, Liu Qiaoquan, Zhang Changquan. Study on Physicochemical Properties of Local Waxy Rice Varieties Yaxuenuo and Suyunuo from Taihu Lake Area [J]. Crops, 2020, 36(4): 91-98.
[13]	Song Xiao, Huang Chenchen, Huang Shaomin, Zhang Keke, Yue Ke, Zhang Shuiqing, Guo Doudou, Zhang Yuting. Effects of Tillage and Organic Fertilization Modes on Soil Physical and Chemical Properties and Wheat Yield [J]. Crops, 2020, 36(3): 102-108.
[14]	Zhang Yang, Zhang Wei, Zhao Weijun, Shao Rongfeng, Wang Guan, Xue Dingding, Li Jinmei. Variety Screening and Study of Cultivation Technology for Forage Triticale Varieties Based on Principal Component and Grey Relation Analysis [J]. Crops, 2020, 36(3): 117-124.
[15]	Tian Yucong, Duan Menjun, Zhu Jie, Feng Xiangzhao, Gao Zhenzhen, Liu Zhangyong, Chen Fu, Jin Tao. Effects of Meteorological Conditions on Formation of High Quality Ratoon Rice [J]. Crops, 2020, 36(3): 125-131.