Crops ›› 2020, Vol. 36 ›› Issue (4): 37-44.doi: 10.16035/j.issn.1001-7283.2020.04.006

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Analysis of Grain and Quality Traits of Chromosome Arm Substitution Lines of Triticum dicoccoides in the Background of Triticum aestivum

Wang Zhongqiu(), Ying Pengfei, Chen Mengtao, He Qiongying, Hu Xin()   

  1. School of Agriculture and Food Science, Zhejiang A & F University/Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Lin’an 311300, Zhejiang, China
  • Received:2019-11-11 Revised:2019-11-21 Online:2020-08-15 Published:2020-08-11
  • Contact: Hu Xin E-mail:1106977276@qq.com;huxin98@foxmail.com

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Abstract:

To excavate the excellent genetic resources of wild emmer, the wild emmer chromosome arm substitution lines with the common wheat variety Bethlehem (BLH) as the genetic background was chosen. The yield related traits (grain length, grain width and 1000-grain weight) and quality related traits (protein content, wet gluten content, zeleny, starch content, and ash content) were investigated. The results showed that, the grain length of 3AL during two years was significantly longer than that of the parent BLH, suggesting at least one positive QTL for grain length on the 3AL chromosome arm of wild emmer. Similarly, at least three negative QTLs controlling grain length were located on 4BS, 6BL, and 7BL, respectively. At least 11 negative QTLs for 1000-grain weight were located on 2AS, 5AS, 6AL, 7AS, 1BS, 1BL, 4BS, 4BL, 5BL, 6BL and 7BL, respectively. At least six positive QTLs increasing protein content distributed on 6AL, 1BS, 2BS, 3BL, 7BS and 7BL, respectively. At least three positive QTLs responsible for wet gluten content were scattered at 2BL, 7BS and 7BL. At least three major QTLs controlling sedimentation value located at 4AL, 7AL and 7BL. Negative QTL site controlling starch content positioned at 7BL. QTL for ash content of wheat grain was on 7BL. Correlation analysis showed negative correlation between grain weight and protein content, wet gluten content, the zeleny and ash content, while the protein content was positively correlated with the wet gluten content, the zeleny, and the ash content and negatively correlated with starch content. Results showed that CASLs group are rich in genetic diversity, and each substitution lines only contains the chromosome arms corresponding to wild emmer, each of which has different genetic characteristics, so it can comprehensively utilize the favorable traits of the substitution lines.

Key words: Triticum dicoccoides, Chromosome arm substitution lines, Grain trait, Quality trait, Correlation analysis

Table 1

Genetic variation of grain traits and quality traits in wheat CASLs population"

性状Trait 年份
Year		 亲本Parent BLH CASLs群体CASLs population
最小值Min 最大值Max 变幅Range 平均数Mean 变异系数Variable coefficient (%)
粒长Grain length (mm) 2018 6.80 5.90 7.30 1.40 6.50 4.60
2019 6.70 5.90 7.50 1.50 6.60 4.80
均值Mean 6.75 5.90 7.38 1.47 6.55 4.70
粒宽Grain width (mm) 2018 3.30 2.90 3.60 0.70 3.30 5.20
2019 3.20 2.80 3.70 0.90 3.20 6.80
均值Mean 3.25 2.86 3.66 0.81 3.25 6.00
千粒重1000-grain weight (g) 2018 42.13 32.20 44.70 12.50 38.83 7.30
2019 41.39 29.05 42.74 13.69 38.11 7.40
均值Mean 41.76 30.63 43.72 13.10 38.47 7.35
蛋白质含量Protein content (%) 2018 13.53 12.24 18.55 6.31 13.89 8.30
2019 13.43 12.38 16.99 4.61 13.73 6.60
均值Mean 13.48 12.31 17.77 5.46 13.81 7.45
湿面筋含量Wet gluten content (%) 2018 26.75 24.18 38.99 14.81 28.44 9.50
2019 27.45 24.20 35.87 11.67 28.04 7.40
均值Mean 27.10 24.19 37.43 13.24 28.24 8.45
沉降值Zeleny (mL) 2018 40.55 32.15 62.75 30.60 41.32 13.10
2019 40.99 32.43 51.65 19.22 40.63 9.90
均值Mean 40.77 32.29 57.20 24.91 40.98 11.50
淀粉含量Starch content (%) 2018 58.42 53.11 60.72 7.61 58.23 2.20
2019 58.25 53.12 61.56 8.44 58.35 2.60
均值Mean 58.34 53.12 61.14 8.03 58.29 2.40
灰分含量Ash content (%) 2018 0.51 0.48 0.56 0.08 0.51 3.40
2019 0.52 0.49 0.59 0.10 0.53 3.40
均值Mean 0.52 0.49 0.58 0.09 0.52 3.40

Fig.1

Differences in grain traits relative to parent in wheat CASLs population "*" indicates significant difference between the CASLs and the parent at 0.05 level. The same below"

Fig.2

Differences in quality traits relative to parent in wheat CASLs population"

Table 2

Correlation analysis among major traits"

2018年
In 2018		 粒长
Grain length		 粒宽
Grain width		 千粒重
1000-grain weight		 蛋白质含量
Protein content		 湿面筋含量
Wet gluten content		 沉降值
Zeleny		 淀粉含量
Starch content		 灰分含量
Ash content		 2019年
In 2019
粒长 -1 -0.81** -0.61** -0.27* -0.26* -0.15 -0.27* -0.18 粒长
粒宽 -0.57** -1 -0.43** -0.25* -0.25* -0.15 -0.33** -0.20 粒宽
千粒重 -0.47** -0.41* -1 -0.48** -0.46** -0.39** -0.16 -0.28 千粒重
蛋白质含量 -0.18 -0.38* -0.47** -1 -0.96** -0.84** -0.68** -0.55** 蛋白质含量
湿面筋含量 -0.15 -0.39* -0.55** -0.96** -1 -0.87** -0.64** -0.56** 湿面筋含量
沉降值 -0.23* -0.12 -0.56** -0.88** -0.89** -1 -0.58** -0.45** 沉降值
淀粉含量 -0.20 -0.31* -0.27* -0.77** -0.77** -0.66** -1 -0.65** 淀粉含量
灰分含量 -0.17 -0.22 -0.33* -0.68** -0.67** -0.60** -0.63** -1 灰分含量
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