Crops ›› 2020, Vol. 36 ›› Issue (4): 84-90.doi: 10.16035/j.issn.1001-7283.2020.04.012

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Isolation and Expression Analysis of Transcription Factor Gene TaMYB70 in Wheat

Xu Yuanyuan1(), Zhao Peng2, Hong Quanchun1, Zhu Xiaoqin1, Pei Dongli1()   

  1. 1School of Biology and Food Sciences, Shangqiu Normal University/Henan Provincial Key University Laboratory of Plant-Microbe Interactions, Shangqiu 476000, Henan, China
    2School of Humanities, Shangqiu Normal University, Shangqiu 476000, Henan, China
  • Received:2019-12-16 Revised:2020-03-03 Online:2020-08-15 Published:2020-08-11
  • Contact: Pei Dongli E-mail:xuyy1108@163.com;peidongli@126.com

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Abstract:

To excavate the function of MYB transcription factor TaMYB70 in wheat, TaMYB70 gene was isolated using homology cloning strategy. The expression patterns of TaMYB70 under different stresses were examined by quantitative real-time PCR (qRT-PCR). The results showed that the TaMYB70 partial cDNA was 1 272bp and contained an open reading frame of 1 108bp, encoding 335 amino acids. TaMYB70 protein contained two Myb-type HTH DNA binding domains which were helix-turn-helix motifs. The homologous sequence alignment indicated that the TaMYB70 had higher homology with MYB44 from other plants, such as Aegilops tauschii and Brachypodium distachyum (L.). TaMYB70 belongs to the same branch as the 22nd members of the arabidopsis R2R3-MYB transcription factors. The expression of TaMYB70 was upregulated under ABA treatment, and downregulated under PEG and NaCl stresses, and which may be involved in stress response of wheat.

Key words: Wheat, TaMYB70, Transcription factor, R2R3-MYB, Expression analysis

Table 1

The primers used in this study for qRT-PCR"

引物名
Primer name		 引物序列(5′-3′)
Primer sequence (5′-3′)		 产物长度
Length of PCR
product (bp)
ACTIN-F TCTGTCCTTGTATGCCAGCG 195
ACTIN-R GCGGTTGTTGTGAGGGAGTA
MYB-YG-F GGTCCCTCATCAGCAAGTCC 213
MYB-YG-R TCCAGTGGTTCTTGATGGCG

Fig.1

The amplification results of wheat TaMYB70 gene"

Fig.2

Functional domain prediction of wheat TaMYB70 protein"

Fig.3

Three-dimensional structure model of wheat TaMYB70 protein"

Fig.4

Multiple sequence alignments of wheat TaMYB70 protein"

Fig.5

Sequence analysis of wheat TaMYB70 protein domain"

Fig.6

Phylogenetic tree of TaMYB70 and the MYB transcription factors in Arabidopsis"

Fig.7

Expression characteristics of wheat TaMYB70 gene under abiotic stress"

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