Crops ›› 2021, Vol. 37 ›› Issue (3): 28-33.doi: 10.16035/j.issn.1001-7283.2021.03.004

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Difference in Nitrogen Absorption and Transportation and Utilization of Rapeseed Germplasms with Contrasting Nitrogen Efficiency

Qin Lu1(), Wang Jianqiang1,2(), Han Peipei1,3, Li Yinshui1, Gu Chiming1, Hu Xiaojia1, Xie Lihua1, Liao Xing1()   

  1. 1Oil Crops Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetics Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, Hubei, China
    2Qingdao Kingagroot Compound Co., Ltd., Qingdao 266000, Shandong, China
    3Institute of Agriculture Sciences in Jiangsu Coastal Area, Yancheng 224002, Jiangsu, China
  • Received:2020-06-18 Revised:2020-07-31 Online:2021-06-15 Published:2021-06-22
  • Contact: Liao Xing E-mail:123@126.com;18615083316@163.com;liaox@oilcrops.cn

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Abstract:

The study of nitrogen (N) efficiency is the prerequisite for genetic improvement of nutritional traits in rapeseed (Brassica napus L.). In order to investigate the possible differences in N absorption, transportation, and utilization of rapeseed with different N efficiency at seedling stage, two rapeseed germplasms with contrasting N efficiency, H6 (N-efficient germplasm) and L18 (N-inefficient germplasm), were used as the materials. By using hydroponic cultures with normal N (CK) and low N (LN) treatments for 14 days, the N content, N accumulation, N transport coefficient, and N utilization efficiency were measured. The results showed that the N absorption, transportation, and utilization efficiency in rapeseed seedlings at two N levels reached extremely significant levels (P<0.01). Compared with CK conditions, the biomass, N content, and N accumulation of rapeseed were significantly decreased under LN, while the N utilization efficiency and root-shoot ratio were significantly increased. In addition, the biomass, N accumulation, N transport coefficient, and N utilization efficiency of N-efficient rapeseed germplasm H6 were significantly higher than that of N-inefficient germplasm L18 under LN conditions. In detail, the biomass, N accumulation, N transport coefficient, and N physiological utilization rate of H6 were 2.07, 1.42, 3.23, and 1.56 times as much as L18, respectively. The possible mechanisms underlying rapeseed with different N efficiency at seedling stage were elucidated from the absorption, transportation, and utilization process of N. These results may provide useful information for investigating the mechanism underlying N efficiency and genetic improvement of N efficiency in rapeseed breeding.

Key words: Rapeseed, Low N stress, N efficiency, Absorption and transportation, Utilization

Table 1

The formula of nutrient solution"

化学试剂
Chemical agent		 浓度Concentration (μmol/L)
CK LN
Ca(NO3)2·4H2O 2500 50
KNO3 2500 50
NH4NO3 1000 20
KH2PO4 500 500
MgSO4·7H2O 1000 1000
EDTA-Fe 40 40
K2SO4 250 1250
CaCl2 0 2500
MnCl2·4H2O 4.5 4.5
ZnSO4·7H2O 0.3 0.3
CuSO4·5H2O 0.16 0.16
H3BO3 20 20
(NH4)6Mo7O24·4H2O 0.16 0.16

Table 2

Comparison of biomass and root-shoot ratio in rapeseed germplasms with contrasting N efficiency at different N levels"

种质编号
Germplasm number		 处理
Treatment		 地上部干重
Shoot dry weight (g)		 根干重
Root dry weight (g)		 生物量
Biomass dry weight (g)		 根冠比
Ratio of root to shoot
H6 CK 0.232±0.022a 0.036±0.003a 0.269±0.024a 0.158±0.010c
LN 0.080±0.016b 0.033±0.003a 0.114±0.018b 0.425±0.009b
LN/CK 0.34 0.92 0.42 2.69
L18 CK 0.224±0.018a 0.033±0.003a 0.257±0.020a 0.155±0.009c
LN 0.035±0.015c 0.019±0.003b 0.055±0.017c 0.541±0.010a
LN/CK 0.16 0.58 0.21 3.49

Fig.1

Comparison of N accumulation in rapeseed germplasms with contrasting N efficiency at different N levels Different lowercase letters indicate significant difference at 5% level, the same below"

Table 3

Comparison of N content and transport coefficient in rapeseed germplasms with contrasting N efficiency at different N levels"

种质编号
Germplasm number		 处理
Treatment		 地上部氮含量
Shoot N content (mg/g)		 根系氮含量
Root N content (mg/g)		 整株氮含量
Total N content (mg/g)		 转运系数
Transport coefficient
H6 CK 36.70±0.15a 50.48±0.01a 38.56±0.13a 0.72±0.01b
LN 14.91±0.05c 28.59±0.05b 18.98±0.05d 0.52±0.01c
LN/CK 0.40 0.56 0.49 0.72
L18 CK 36.49±0.34a 29.46±0.05b 35.58±0.28b 1.23±0.01a
LN 17.63±0.11b 46.05±0.52a 27.61±0.11c 0.38±0.01d
LN/CK 0.48 1.56 0.77 0.31

Fig.2

Comparison of nitrogen utilization efficiency in rapeseed germplasms with contrasting Nefficiency at different N levels"

Table 4

Comparison of dry weight utilization and N utilization in roots of rapeseed germplasms with contrasting N efficiency at different N levels"

处理
Treatment		 种质编号
Germplasm
number		 根系干物质利用效率
Root dry matter
utilization (cm2/mg)		 根系氮利用效率
Root N utilization
(cm2/mg)
CK H6 1.24±0.43b 24.58±8.69c
L18 1.30±0.46b 43.40±15.34b
LN H6 1.56±0.55a 55.26±19.35a
L18 1.27±0.44b 27.59±9.75c
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