Crops ›› 2022, Vol. 38 ›› Issue (1): 154-160.doi: 10.16035/j.issn.1001-7283.2022.01.023

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Evaluation of Wheat Freezing Damage during Overwintering Period Based on Chlorophyll Fluorescence

Yang Cheng(), Du Simeng, Zhang Deqi, Shi Yanhua, Li Xiangdong(), Shao Yunhui, Fang Baoting, Wang Hanfang   

  1. Wheat Research Institute, Henan Academy of Agricultural Sciences/National Laboratory of Wheat Engineering/Key Laboratory of Wheat Biology and Genetic Breeding in Central Huang-Huai Region, Ministry of Agriculture and Rural Affairs/Scientific Observing and Experimental Station of Crop Cultivation in Central Plains, Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory of Wheat Biology, Zhengzhou 450000, Henan, China
  • Received:2021-01-13 Revised:2021-05-07 Online:2022-02-15 Published:2022-02-16
  • Contact: Li Xiangdong E-mail:luckytiger.com@163.com;hnlxd@126.com

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Abstract:

Freezing damage is a common natural disaster in wheat production. At present, the methods for non-destructive evaluation of freezing damage of wheat are still very limited. Rapid chlorophyll fluorescence induction kinetics curve contains abundant information about photosynthesis, which is a common method to study environment stress. By screening suitable chlorophyll fluorescence parameters to evaluate the cold resistance of wheat, it provides technical support for the cold resistance mechanism, overwintering management and cold resistance breeding of wheat. In this study, by investigating the correlation between 34 commonly used chlorophyll fluorescence induction kinetic parameters and frost damage degree of 33 wheat varieties in the year of freezing injury occurrence in overwintering period, TRo/CSm was screened out as an indicator with significant positive correlation with frost damage degree of wheat, and the correlation coefficient reached 0.636, which correlation was better than the previous chlorophyll fluorescence index Fv/Fm. It indicates that the chlorophyll fluorescence parameter TRo/CSm can be used to evaluate the cold resistance of wheat. The findings of this study provided strong support for winter wheat overwintering management and cold resistance breeding.

Key words: Wheat, Freezing damage, Cold resistance, Chlorophyll fluorescence

Table 1

The wheat varieties tested"

编号
Number		 品种
Variety		 编号
Number		 品种
Variety
1 碧玛1号Bima 1 18 矮抗58 Aikang 58
2 碧玛4号Bima 4 19 郑麦366 Zhengmai 366
3 阿夫Afu 20 周麦22 Zhoumai 22
4 阿勃Abo 21 郑麦7698 Zhengmai 7698
5 郑州7023 Zhengzhou 7023 22 矮丰3号Aifeng 3
6 丰产3号Fengchan 3 23 郑麦0856 Zhengmai 0856
7 郑州761 Zhengzhou 761 24 平原50 Pingyuan 50
8 西安8号Xi’an 8 25 葫芦头Hulutou
9 百农3217 Bainong 3217 26 大口麦Dakoumai
10 宝丰7228 Baofeng 7228 27 红和尚头Hongheshangtou
11 豫麦13 Yumai 13 28 西农6028 Xinong 6028
12 周麦9号Zhoumai 9 29 白玉皮Baiyupi
13 豫麦18 Yumai 18 30 偃展4110 Yanzhan 4110
14 豫麦49 Yumai 49 31 内乡5号Neixiang 5
15 郑麦9023 Zhengmai 9023 32 博爱7023 Boai 7023
16 兰考矮早8 Lankaoaizao 8 33 豫麦2号Yumai 2
17 周麦18 Zhoumai 18

Table 2

Parameters and formula of O-J-I-P curve"

参数
Parameter		 计算方法
Method of calculation
Fm 暗适应后照光获得的最大荧光强度
Fo 叶绿素荧光诱导动力学曲线20μs的荧光强度
Fv 可变荧光,Fm-Fo
Area 叶绿素荧光诱导动力学曲线到Y轴的面积
t(Fm) 从暗适应后开始照光至达到最大荧光的时间
ϕPo ϕPo=Fv/Fm=(Fm-Fo)/Fm
Wk Wk=(F300μs-Fo)/(F2ms-Fo)
VJ VJ=(F2ms-Fo)/(Fm-Fo)
VI VI=(F30ms-Fo)/(Fm-Fo)
Ψo ψO=ETo/TRo=(1-VJ)
δRo δRo=(1-VI)/（1-VJ）
ϕEo ϕEo=ETo/ABS
ϕDo ϕDo=1-ϕPo
ϕRo ϕRo=ϕPo(1-VI)
Sm Sm=Area/(Fm-Fo)
Mo Mo=4(F300μs-Fo)/(Fm-Fo)
N N=SmMo(1/VJ)
ABS/RC ABS/RC=Mo(1/VJ)(1/ϕPo)
TRo/RC TRo/RC=Mo(1/VJ)
ETo/RC ETo/RC=Mo(1/VJ)ψO
DIo/RC DIo/RC=(ABS/RC)-(TRo/RC)
ABS/CSo ABS/CSo=Fo
TRo/CSo TRo/CSo=Fm
ETo/CSo ETo/CSo=φEo(ABS/CSo)
DIo/CSo DIo/CSo=(ABS/CSo)-(TRo/CSo)
RC/CSo RC/CSo=φPo(VJ/Mo)(ABS/CSo)
ABS/CSm ABS/CSm=Fm
TRo/CSm TRo/CSm=φPo(ABS/CSm)
ETo/CSm ETo/CSm=φEo(ABS/CSm)
DIo/CSm DIo/CSm=(ABS/CSo)-(TRo/CSo)
RC/CSm RC/CSm=φPo(VJ/Mo)(ABS/CSm)
PIabs PIabs=(RC/ABS)[φPo/(1-φPo)][ψO/(1-ψO)]
PItatal PItatal=(RC/ABS)[φPo/(1-φPo)][ψO/(1-ψO)][ϕRo/ABS(1-ϕRo)]
VIP VIP=1-VI

Fig.1

Temperature change during wheat growth period in 2016-2017"

Fig.2

Distribution of daily minimum temperature frequencies from November 1st to December 15th in 2016"

Table 3

Investigation on freezing damage of different wheat varieties during over winter"

编号
Number		 品种
Variety		 株高
Plant height
(cm)		 单株分蘖数
Tillers number
per plant		 单株叶片数
Leaves number
per plant		 单株受冻叶片数
Frozen leaves
per plant		 叶片冻伤率
Leaf frostbite
rate
1 碧玛1号 21.26±4.09 9.25±1.50 27.00±4.58 3.20±2.39 0.12±0.10
2 碧玛4号 21.24±3.04 7.40±1.14 23.00±5.87 2.80±1.10 0.13±0.06
3 阿夫 20.50±2.47 11.00±0.71 29.80±2.59 4.40±1.52 0.15±0.06
4 阿勃 26.80±2.49 7.00±1.58 23.80±4.92 6.40±0.89 0.28±0.05
5 郑州7023 31.56±2.07 7.20±1.48 24.40±2.88 7.20±2.28 0.29±0.07
6 丰产3号 16.40±1.08 8.60±1.52 30.00±6.28 3.00±1.22 0.10±0.05
7 郑州761 22.70±3.63 6.00±1.58 22.40±5.03 5.60±1.95 0.24±0.04
8 西安8号 23.50±1.00 9.00±2.24 26.40±6.80 5.00±2.00 0.19±0.07
9 百农3217 22.40±3.14 8.40±1.14 24.20±1.30 4.40±1.67 0.18±0.06
10 宝丰7228 22.30±3.77 8.80±0.84 26.40±1.52 6.80±1.30 0.26±0.05
11 豫麦13 18.90±1.52 8.80±0.45 25.00±2.65 3.80±1.10 0.15±0.05
编号
Number		 品种
Variety		 株高
Plant height
(cm)		 单株分蘖数
Number of tillers
per plant		 单株叶片数
Number of leaves
per plant		 单株受冻叶片数
Frozen leaves
per plant		 叶片冻伤率
Leaf frostbite
rate
12 周麦9号 21.76±2.94 8.20±1.64 23.20±4.71 5.40±1.14 0.24±0.05
13 豫麦18 23.70±2.36 8.80±0.45 25.00±2.65 3.80±1.10 0.15±0.05
14 豫麦49 22.10±1.78 6.80±2.05 26.80±6.30 7.00±1.87 0.27±0.05
15 郑麦9023 19.36±1.97 9.80±2.17 34.40±2.51 3.80±0.45 0.11±0.02
16 兰考矮早8 24.38±1.25 7.33±1.53 24.60±3.44 5.40±1.14 0.26±0.07
17 周麦18 27.56±1.64 7.60±2.51 21.80±5.93 5.00±1.41 0.24±0.07
18 矮抗58 20.60±1.08 7.40±1.14 23.00±5.87 2.80±1.10 0.13±0.06
19 郑麦366 24.40±1.67 8.80±0.45 25.40±3.21 5.00±2.00 0.19±0.06
20 周麦22 23.80±2.20 8.60±1.67 21.60±2.70 4.20±1.64 0.20±0.08
21 郑麦7698 23.24±2.37 7.60±1.52 25.60±6.31 3.20±0.84 0.13±0.04
22 矮丰3号 18.80±1.15 7.40±2.07 27.80±5.45 6.20±1.10 0.23±0.06
23 郑麦0856 18.76±2.95 11.00±1.41 30.40±3.21 5.40±1.14 0.18±0.05
24 平原50 21.80±1.60 8.80±0.45 28.80±3.56 3.80±0.84 0.13±0.02
25 葫芦头 16.90±2.53 8.80±1.79 34.40±3.85 1.60±0.89 0.05±0.02
26 大口麦 20.40±2.27 9.20±1.92 26.20±5.85 3.60±1.34 0.14±0.03
27 红和尚头 23.80±2.51 8.20±1.92 22.80±2.49 3.20±1.30 0.14±0.06
28 西农6028 20.90±3.01 7.20±1.30 27.20±3.19 2.60±1.34 0.10±0.05
29 白玉皮 17.90±3.47 9.40±4.34 32.40±4.83 1.80±0.45 0.06±0.01
30 偃展4110 23.70±2.36 7.80±1.64 24.20±2.77 8.60±2.07 0.35±0.06
31 内乡5号 28.50±1.94 6.00±0.71 16.80±2.05 6.60±2.30 0.41±0.17
32 博爱7023 26.30±1.60 6.60±2.79 32.60±5.41 4.80±1.30 0.15±0.05
33 豫麦2号 22.90±1.24 7.40±1.52 25.60±3.21 6.60±0.89 0.26±0.04
最大值Maximum 31.56 11.00 34.40 8.60 0.41
最小值Minimum 16.40 6.00 16.80 1.60 0.05
平均值Average 22.40 8.18 25.82 4.61 0.19
变异系数Coefficient of variation 0.15 0.15 0.15 0.37 0.43

Fig.3

Histogram of leaf frostbite rates of wheat varieties"

Fig.4

Correlation between leaf frostbite rates and growth indexes of wheat"

Table 4

Seven fluorescence parameters with the strongest correlation with frostbite rates of wheat"

相关性
Correlation		 参数
Parameter		 相关系数
Correlation coefficient		 P值
P value
正相关 TRo/CSm 0.636 0.000
Positive correlation Fm 0.604 0.000
ETo/CSm 0.601 0.000
RC/CSm 0.589 0.000
Fv/Fm 0.582 0.000
负相关 DIo/RC -0.492 0.004
Negative correlation ϕDo -0.582 0.000

Fig.5

Correlation between chlorophyll fluorescence parameters and plant height of wheat"

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