Crops ›› 2023, Vol. 39 ›› Issue (1): 38-45.doi: 10.16035/j.issn.1001-7283.2023.01.006

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Research on the Effects of Gene Expression in Sugar Metabolism Pathway of Potato by Drought Stress and Rehydration

Liu Sujun1,2(), Meng Meilian3, Suriguga 1,2()   

  1. 1College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, Inner Mongolia, China
    2Key Laboratory of Biodiversity Conservation and Sustainable Utilization for College and University of Inner Mongolia Autonomous Region, Hohhot 010022, Inner Mongolia, China
    3College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010020, Inner Mongolia, China
  • Received:2021-09-02 Revised:2021-12-15 Online:2023-02-15 Published:2023-02-22

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Abstract:

High-throughput sequencing technique was used to study gene expression in potato metabolic pathway to clarify the damage of potato under drought stress and the protective effects of rehydration on potato. The experiment was carried out by pot method, in which drought treatment (40% soil relative water content, DT) and drought control (keep soil relative water content 70% for 14 days, CK) were set up, and in which rehydration treatment (the relative soil water content was 40% after being maintained for 14 days and then recovered to 70% of soil relative water content, RT) and rehydration control (relative soil water content was maintained at 70% for 21 days, RCK) were set up, and the effects of drought stress and rehydration treatment on starch and sugar metabolism pathways of potato were studied. The DT and CK treatments showed significant changes in the expression of 655 genes, of which 13 genes related to starch and sucrose metabolism pathways, including nine up-regulated genes and four down-regulated genes, and there were 11 enzymes involved. There were 644 differentially expressed genes between RT and RCK treatments, of which five genes related to starch and sucrose metabolism pathways, including three up-regulated genes and two down-regulated genes, and there were four enzymes involved. Changes in the expression of 13 genes in starch and sucrose metabolic pathways were occurred after drought stress, involving 11 enzymes in metabolic pathways, their functions were related to glycosyl hydrolase, sucrose synthase, glycosyl transferase, starch synthase and pectin esterase. After rehydration, the expression of five genes was still significantly different, affecting four enzymes. The number of genes with differential expression was reduced by eight, and the number of enzymes involved was reduced by seven, the decline was more obvious. Although it was not completely repaired, the overall repair effects was better and the compensation effect was significant.

Key words: Potato, Gene expression, High-throughput sequencing, Starch, Sugar metabolism

Fig.1

Test of RNA-Seq correlation between different treatments"

Table 1

Data of test summary"

处理
Treatment		 原始数据
Raw read		 处理后的数据
Clean read		 Q30
(%)		 匹配数（匹配率）
Total mapped
(rate of the mapped)		 交叉匹配数（交叉匹配率）
Multiply mapped
(rate of the multiply mapped)		 精准匹配数（精准匹配率）
Uniquely mapped
(rate of the uniquely mapped)
DT 52 481 881 49 863 309 92.03 31 377 909（62.93%） 1 101 135（2.21%） 30 276 774（60.72%）
CK 51 325 932 49 651 786 94.38 32 523 133（65.50%） 1 083 605（2.18%） 31 439 528（63.32%）
RT 45 604 570 44 071 743 94.25 28 817 595（65.39%） 912 019（2.07%） 27 905 575（63.32%）
RCK 47 472 519 45 593 043 94.17 29 233 720（64.12%） 977 935（2.14%） 28 255 785（61.97%）

Table 2

Number statistics of differential expression genes between different treatment groups"

项目Item DT vs CK RT vs RCK
差异表达基因总数Total number of differentially expressed genes 655 644
上调基因表达数量Number of up-regulated genes 349 149
下调基因表达数量Number of down-regulated genes 306 495

Fig.2

Venn diagram of DT vs CK and RT vs RCK"

Table 3

Statistics of gene expression with significant differences in starch and sucrose metabolism pathways"

表达发生变化的基因
Genes changed expression		 对应的酶编码
Correspond
enzyme code		 上调还是下调
Up-regulated or
down-regulated		 P值
P-value		 log2FoldChange 功能描述
Functional description
PGSC0003DMG402028252 3.2.1.26 下调 0.020165 -0.81723 糖基水解酶，C、N-末端
PGSC0003DMG400013546 2.4.1.13 上调 1.96E-06 0.78233 蔗糖合酶||糖基转移酶
PGSC0003DMG400006319 3.2.1.21 上调 0.044167 0.93775 糖苷水解酶，催化结构域，活性位点
PGSC0003DMG401017546 2.4.1.15、3.1.3.12 上调 0.021277 0.57240 海藻糖磷酸酶||糖基转移酶
PGSC0003DMG400009026
 2.7.7.27
 上调
 0.041903
 0.41162
 ADP-葡萄糖焦磷酸化酶||核苷酸-二磷酸-糖转移
酶||核苷酸基转移酶||葡萄糖-1-磷酸腺苷基转移酶
PGSC0003DMG402013540 2.4.1.21 上调 8.06E-06 1.75040 淀粉合酶，催化结构域
Novel02720 2.4.1.18 上调 0.000945 1.37100 1,4-α-葡聚糖支化酶2-2，叶绿素|淀粉分支酶II
PGSC0003DMG400007782 2.4.1.1 上调 0.014013 0.51617 糖基转移酶，家族35
PGSC0003DMG400033858 2.4.1.1 上调 0.028031 0.91992
Novel02357 5.4.2.2 上调 0.000539 0.71058 磷酸葡萄糖突变酶
PGSC0003DMG400009178 3.1.1.11 下调 0.040198 -0.85467 果胶酯酶抑制剂
PGSC0003DMG400030191 3.1.1.11 下调 0.002582 -1.41130 果胶裂解酶倍数||果胶酯酶，催化
PGSC0003DMG400002085 3.1.1.11 下调 0.005801 -1.46070 果胶酯酶抑制剂||果胶裂解酶倍数

Fig.3

Effects of drought stress on starch and sugar metabolism pathways Red indicates significant up-regulate of gene expression, green indicates significant down-regulation of gene expression. SSM: starch and sucrose metabolism; ASNSM: amino sugar and nucleotide sugar metabolism; UDGA: udp-d-galacturonate; PEC: pectin; PET: pectate; DGT: d-galacturonate; ASM: ascorbate metabolism; DGN: d-glucuronate; βDGS: β-d-glucuronoside; REM: retinol metabolism; UDGL: udp-d-glucuronate; UDX: udp-d-xylose; βDXL: 1,4-β-d-xylan; DXY: d-xylose; PGI: pentose and glucuronate interconversions; SUCE: sucrose (extracellular); αDG6P: α-d-glucose-6P; βDFSN: (2,6-β-D- Fructosyl)n; βDFSM: (2,6-β-d-fructosyl)m+n; βDFR: β-d-fructose; KET: 3-ketosucrose; SUC: sucrose; SUP: sucrose-6p; DFR: d-fructose; DGL: d-glucose; βGLU: 1,3-β-glucan; αDGL: α-d-glucose; βDGC: 1,4-β-d-glucan; CEL: cellulose; GLU: glucoside; βDG: β-d-glucose; GGL: gdp-glucose; CGL: cdp-glucose; αDGLP: α-d-glucose-1,6p2; βDFP: β-d-fructose-6p; αDGP: α-d-glucose-1p; ADPG: adp-glucose; DFT: d-fructose; UGL: udp-glucose; GLA: glycogen amylose; GLY: glycolysis; GLN: gluconeogenesis; αDG6P: α-d-glucose-6p; THL: trehalose; βDG6P: β-d-glucose-6p; βDGP: β-d-glucose-1p; DG6P: d-glucose-6p; TRP: trehalose-6p; TRE: trexyz; THLE: α,α-trehalose (extracellular); MAT: maltose; MAP: maltose-6'p; CYR: cyrclomaltodextrin; MALE: maltose(extracellular); MAL: maltodextrin; STG: starch glycogen; DEX: dextrin; α-MAP: α-maltose-1-p; ISO: isomaltose; CEB: cellobiose; CELL: cellotriose; CER: cellotetraose; CEP: cellopentaose; CEX: cellohexaose ; CET: celloheptaose. The same below"

Table 4

Statistics of gene expression with significant differences after rehydration"

表达发生变化的基因
Genes changed expression		 对应酶编码
Correspond
enzyme code		 上调还是下调
Up-regulated or
down-regulated		 P值
P-value		 log2FoldChange 功能描述
Functional description
PGSC0003DMG402028252 3.2.1.26 下调 0.020165 -0.81723 糖基水解酶，C、N-末端
PGSC0003DMG400026428 2.4.1.14 上调 0.007217 0.82062 蔗糖磷酸合酶||糖基转移酶
PGSC0003DMG400009177 3.1.1.11 下调 0.002437 -0.70394 果胶裂解酶||果胶酯酶抑制剂
Novel02278 2.4.1.18 上调 0.021939 0.92412 1,4-α-葡聚糖支化酶3，叶绿体/淀粉体
PGSC0003DMG400022307 2.4.1.18 上调 0.010419 0.88644 α-淀粉酶||糖苷水解酶||糖基水解酶

Fig.4

Effects of rehydration on starch and sugar metabolism pathways"

Fig.5

Histogram presentation of ten different expression genes 1：PGSC0003DMG400014867， 2：PGSC0003DMG400030713， 3：PGSC0003DMG400019063， 4：PGSC0003DMG400001774， 5：PGSC0003DMG400024278， 6：PGSC0003DMG400013702， 7：PGSC0003DMG400006448， 8：Nove101381， 9：PGSC0003DMG 400003563， 10：PGSC0003DMG400000425"

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