Crops ›› 2023, Vol. 39 ›› Issue (1): 196-200.doi: 10.16035/j.issn.1001-7283.2023.01.029

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Effects of EDTA on Growth and Physiological Characteristics of Cucumber Seedlings under Chromium Stress

Zhang Panpan(), Wu Xiong, Ji Jiangtao, Wang Xiaolin   

  1. College of Life Sciences, Yulin University, Yulin 719000, Shaanxi, China
  • Received:2021-09-15 Revised:2021-11-12 Online:2023-02-15 Published:2023-02-22

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Abstract:

In order to explore the physiological regulation mechanism of ethylenediamine tetraacetic acid (EDTA) under alleviating chromium stress in cucumber seedlings, “Hengyou Vegetable Garden” was used as test materials in soil culture with 120mg/L chromium ion solution to simulate chromium stress, the effects of different concentrations of EDTA (W1: 1mmol/L, W2: 2mmol/L and W3: 4mmol/L) on seedling growth and physiological characteristics under chromium stress were studied. The results showed that, W2 and W3 treatments promoted the increase of plant height and dry matter weight of shoot in seedling. Meanwhile, W2 treatment increased the photochemical and non-photochemical quenching coefficients of leaf photosystem. Peroxidase (POD) activity in leaves and roots of seedlings was significantly increased after the treatments, while malondialdehyde (MDA) content in leaves was significantly decreased under W2 and W3 treatments, and MDA content in roots was significantly decreased under W3 treatment. The content of soluble protein in seedling leaves decreased, while the content of proline had been increased under the treatments. In conclusion, 1-2mmol/L EDTA under chromium stress could improve photosynthesis of cucumber seedlings, enhance POD activity and osmotic regulation ability, slow down the level of membrane lipid peroxidation of seedlings, and promote shoot morphological development of seedlings, thus effectively alleviating the harm of chromium stress on seedlings.

Key words: EDTA, Cucumis sativus L., Leaf, Chromium stress, Physiological characteristics

Table 1

Effects of EDTA on morphological characteristics of cucumber seedlings under chromium stress"

处理
Treatment		 株高
Plant height (cm)		 茎粗
Stem diameter (mm)		 绿叶面积
Area of green leaves (cm2)		 总根长
Total length of root (cm)		 根平均直径
Mean diameter of root (mm)
CK 8.20±0.36c 4.01±0.26a 164.32±9.00a 1649.19±24.01a 0.28±0.02a
W1 7.87±0.29c 3.55±0.44a 105.76±8.09c 962.35±38.88b 0.31±0.02a
W2 9.07±0.60b 4.21±0.22a 126.85±11.45b 1089.04±94.14b 0.30±0.01a
W3 10.47±0.21a 3.83±0.76a 171.15±9.39a 1696.37±39.73a 0.28±0.01a

Table 2

Effects of EDTA on dry matter accumulation of cucumber seedlings under chromium stress"

处理
Treatment		 地上部干重
Dry weight of
shoot (g)		 地下部干重
Dry weight of
root (g)		 根冠比
Root-shoot
ratio
CK 0.675±0.024b 0.056±0.004a 0.083±0.008b
W1 0.561±0.022c 0.053±0.002a 0.094±0.002a
W2 0.749±0.029a 0.051±0.005a 0.069±0.006c
W3 0.788±0.045a 0.059±0.003a 0.087±0.005b

Table 3

Effects of EDTA on chlorophyll fluorescence characteristics of cucumber seedlings under chromium stress"

处理Treatment Fv/Fm ΦPSⅡ qP NPQ
CK 0.77±0.01a 0.57±0.01b 0.78±0.04b 0.28±0.01c
W1 0.74±0.06a 0.63±0.01a 0.83±0.05a 0.35±0.02b
W2 0.77±0.01a 0.55±0.01c 0.77±0.06b 0.34±0.02b
W3 0.76±0.01a 0.54±0.01c 0.77±0.08b 0.40±0.01a

Fig.1

Effects of EDTA on POD activity in leaves and roots of cucumber seedlings under chromium stress Different letters in the figure represent significant difference between different treatments at the 0.05 level, the same below"

Fig.2

Effects of EDTA on MDA content in leaves and roots of cucumber seedling under chromium stress"

Fig.3

Effects of EDTA on soluble protein and Pro contents in leaves of cucumber seedling under chromium stress"

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