Crops ›› 2023, Vol. 39 ›› Issue (1): 201-206.doi: 10.16035/j.issn.1001-7283.2023.01.030

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Effects of Different Nitrogen Sources on the Growth and Development of Tobacco Seedlings

Li Diqin1(), Yao Shaoyun1, Wang Qing1, Yi Ke2, Liu Yiyun1,3, Tang Xiaoming4, Peng Yuanyuan1, Fu Changwu4()   

  1. 1College of Agronomy, Hunan Agricultural University, Changsha 410128, Hunan, China
    2China Tobacco Hunan Industrial Co., Ltd. of China National Tobacco Corporation, Changsha 410014, Hunan, China
    3China Tobacco Guizhou Industrial Co., Ltd. of China National Tobacco Corporation, Guiyang 550001, Guizhou, China
    4Zhangjiajie Branch of Hunan Tobacco Company, Zhangjiajie 427000, Hunan, China
  • Received:2021-08-22 Revised:2022-06-14 Online:2023-02-15 Published:2023-02-22

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Abstract:

In order to explore the effects of different nitrogen forms sources on the quality, growth and development of tobacco seedlings, a new flue-cured tobacco strain HN2146 was used as material. The effects of four different nitrogen forms on the growth and development characteristics of tobacco seedlings were carried out by hydroponic experiments with ammonium nitrate (NH4NO3), calcium nitrate [Ca(NO3)2], ammonium sulfate [(NH4)2SO4)] and urea [CO(NH2)2]. The results showed that the maximum leaf area of tobacco seedling was 100.4cm2 with the calcium nitrate treatment. The highest protein content in tobacco leaves and roots were the treatment with ammonium sulfate, which were 151.452 and 79.367μg/g FW, respectively. The activity of nitrate reductase shown calcium nitrate > ammonium nitrate > ammonium sulfate > urea treatment. The absorption capacity of nitrogen shown ammonium nitrate > ammonium sulfate > calcium nitrate > urea treatment. Through the principal component analysis of the relevant agronomic traits and physiological characteristics of tobacco seedlings in different treatments, the comprehensive scores were expressed as calcium nitrate > ammonium nitrate > ammonium sulfate > urea treatment. Nitrate nitrogen and ammonium nitrogen as the only nitrogen source were beneficial to the growth and development of tobacco seedlings, while urea as the only nitrogen source had the worst effects. The findings of this study served as a theoretical foundation and point of reference for the application of various nitrogen sources during the development of flue-cured tobacco seedlings.

Key words: Flue-cured tobacco, Tobacco seedlings, Nitrogen source form, Growth and development

Table 1

Agronomic characters of tobacco seedlings of the different treatments"

处理后天数
Days after
treatment (d)		 处理
Treatment		 最大叶长
Length of the
maximum leaf (cm)		 最大叶宽
Width of the
maximum leaf (cm)		 最大叶面积
Areas of the
maximum leaf (cm2)		 苗高
Seedling
height (cm)
0 T1 3.57±0.13a 3.45±0.04a 7.82±0.19a 0.44±0.02a
T2 3.43±0.06a 3.39±0.11a 7.38±0.30a 0.44±0.01a
T3 3.45±0.15a 3.42±0.02a 7.49±0.33a 0.47±0.03a
T4 3.64±0.06a 3.45±0.04a 7.97±0.05a 0.52±0.02a
5 T1 6.14±0.07a 5.13±0.05a 20.00±0.01a 0.82±0.02a
T2 5.44±0.11b 4.99±0.07a 17.23±0.27b 0.90±0.03a
T3 5.17±0.06c 4.48±0.07b 14.69±0.07c 0.80±0.06a
T4 5.18±0.06c 4.52±0.07b 14.87±0.35c 0.90±0.07a
10 T1 10.08±0.14a 7.73±0.11b 49.47±1.28a 1.27±0.06ab
T2 10.12±0.12a 8.03±0.09a 51.61±1.23a 1.36±0.10a
T3 6.70±0.21b 5.33±0.05c 22.68±0.48b 1.13±0.05b
T4 6.65±0.07b 5.35±0.15c 22.57±0.87b 1.15±0.09b
15 T1 13.90±0.73a 10.86±0.33a 95.68±3.93a 2.22±0.04a
T2 14.46±0.46a 10.94±0.10a 100.41±4.09a 2.32±0.16a
T3 8.00±0.14b 6.38±0.13b 32.37±1.08b 1.58±0.10b
T4 7.28±0.18b 5.60±0.25b 25.74±1.51b 1.35±0.10b

Fig.1

Protein content of leaf and root of the different treatments Different lowercase letters indicate significant difference at the 0.05 level, the same below"

Fig.2

NR activity of the different treatments"

Table 2

Nitrogen content in different parts of tobacco seedlings of the different treatments"

部位
Location		 处理
Treatment		 全氮含量
Nitrogen content
(mg/g FW)		 铵态氮含量
Ammonium nitrogen content
(μg/g FW)		 硝态氮含量
Nitrate nitrogen content
(mg/g FW)		 尿素态氮含量
Urea nitrogen content
(mg/g FW)
叶Leaf T1 38.903±0.346a 75.436±2.340b 74.367±1.111b 0.142±0.009b
T2 36.960±0.342a 50.632±1.741c 245.950±2.669a 0.125±0.007b
T3 37.723±0.225a 108.290±1.696a 6.473±0.965c 0.158±0.007b
T4 30.353±0.949b 47.584±1.391c 17.729±1.758c 0.204±0.007a
根Root T1 21.227±0.770a 20.271±1.081b 25.517±1.072b 0.119±0.006b
T2 16.353±0.585b 17.708±0.658c 85.810±1.885a 0.126±0.007b
T3 20.893±0.555a 38.339±0.516a 0.450±0.041c 0.123±0.006b
T4 10.053±0.469c 15.547±0.662d 0.469±0.035c 0.154±0.006a

Table 3

Eigenvalue, variance contribution rate and cumulative variance contribution rate of principal components"

主成分
Principal
component		 特征值
Eigenvalue		 方差贡献率
Variance
contribution rate (%)		 累计方差贡献率
Cumulative variance
contribution rate (%)
1 4.695 58.687 58.687
2 2.986 37.328 96.015

Table 4

Rotated component matrix of the principle component analysis"

指标
Index		 主成分
Principal components
1 2
最大叶面积Areas of the max-leaf (X1) 0.977 -0.091
苗高Seedlings height (X2) 0.977 0.028
蛋白质含量Protein content (X3) -0.168 0.980
硝酸还原酶活性NR activity (X4) 0.983 -0.136
全氮含量Nitrogen content (X5) 0.525 0.841
铵态氮含量Ammonium nitrogen content (X6) -0.206 0.975
硝态氮含量Nitrate nitrogen content (X7) 0.852 -0.341
尿素态氮含量Urea nitrogen content (X8) -0.864 -0.473

Table 5

Different processing principal component scores, comprehensive scores and rankings"

处理
Treatment		 Y1得分
Y1 score		 Y2得分
Y2 score		 综合得分Y
Comprehensive score Y		 均值
Average		 排名
Rank
T1-1 1.52 0.66 1.19 1.10 2
T1-2 1.38 0.56 1.06
T1-3 1.49 0.39 1.06
T2-1 2.65 -1.06 1.21 1.15 1
T2-2 2.56 -0.89 1.22
T2-3 2.34 -1.03 1.03
T3-1 -1.14 2.47 0.27 0.08 3
T3-2 -1.37 2.35 0.08
T3-3 -1.69 2.40 -0.10
T4-1 -2.56 -1.83 -2.28 -2.34 4
T4-2 -2.31 -1.88 -2.15
T4-3 -2.86 -2.15 -2.58
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