Crops ›› 2023, Vol. 39 ›› Issue (4): 230-236.doi: 10.16035/j.issn.1001-7283.2023.04.033

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Analysis of Metabolites and Pathways in Adzuki Bean Seedlings under Fomesafen Stress

Yang Jian1,2(), Tang Huacheng1,2,3,4(), Cao Dongmei1,2,3,4(), Cui Hang1,2, Lou Yuhao1,2, Wang Jifei1,2, Zhang Dongjie1,3,5   

  1. 1College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China
    2Key Laboratory of Agro-Products Processing and Quality Safety of Heilongjiang Province, Daqing 163319, Heilongjiang, China
    3Heilongjiang Engineering Research Center for Coarse Cereals Processing and Quality Safety, Daqing 163319, Heilongjiang, China
    4National Coarse Grain Engineering Research Center, Daqing 163319, Heilongjiang, China
    5Beidahuang Modern Agricultural Industrial Technology Provincial Cultivation Collaborative Innovation Center, Daqing 163319, Heilongjiang, China
  • Received:2022-05-12 Revised:2022-09-15 Online:2023-08-15 Published:2023-08-15

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Abstract:

To explore the regulatory mechanism of field spraying fomesafen (FSA) on the growth and metabolism of adzuki bean seedlings, using the adzuki bean seedlings sprayed with or without FSA as the experimental material, the metabolomics of adzuki bean seedlings was analyzed by liquid chromatography-mass spectrometry (LC-MS). The results showed that compared with the unsprayed adzuki bean seedlings (Z-2-ZZ-2) group, the significantly changed differential metabolites were screened, 106 in the cation mode (50 up-regulated, 56 down-regulated), 130 in anion mode (42 up-regulated, 88 down-regulated), five metabolic pathways with significant differences (pyrimidine metabolism, isoflavone biosynthesis, purine metabolism, galactose metabolism, and arginine and proline metabolism) were annotated in cation mode, mapped to 13 differential metabolites, and annotated to two significantly different metabolic pathways (anthocyanin biosynthesis and flavonoid biosynthesis) in anion mode, mapped to five differential metabolites. Phenylpropane and polyketides, lipids and lipid molecules, and organic acids and their derivatives played a major role in the resistance of adzuki bean seedlings to FSA stress. It provided new ideas for the safety evaluation of the use of FSA in the field of adzuki bean and for increasing the income and yield of adzuki bean.

Key words: Adzuki bean, Seedlings, Fomesafen (FSA), Liquid chromatography-mass spectrometry (LC-MS), Metabolites, Metabolic pathways

Fig.1

Scores of principal component analysis of adzuki bean seedlings in Z-2-ZZ-2 and Z-2-ZZ groups"

Fig.2

OPLS-DA replacement test results of adzuki bean seedlings in Z-2-ZZ-2 and Z-2-ZZ groups"

Fig.3

Volcanic figure of different metabolites of adzuki bean seedlings under FSA stress"

Table 1

"

化合物分类
Classification of compound		 差异代谢物总数量
Total quantity of differential
metabolites		 显著上调
Significant up
regulation		 显著下调
Significant down-
regulation
苯丙烷和聚酮化合物Phenylpropane and polyketone compounds 13 10 3
苯类Benzene 2 2 0
核苷、核苷酸和类似物Nucleosides, nucleotides and analogues 3 0 3
均质非金属化合物Homogeneous non-metallic compound 1 0 1
有机氮化合物Organic nitrogen compounds 1 0 1
有机酸及其衍生物Organic acids and their derivatives 11 0 11
有机氧化合物Organic oxygen compounds 12 11 1
有机杂环化合物Organic heterocyclic compounds 8 2 6
脂质和类脂分子Lipids and lipid molecules 19 8 11
其他Others 36 17 19
合计Total 106 50 56

Table 2

"

化合物分类
Classification of compound		 差异代谢物总数
Total quantity of differential
metabolites		 显著上调
Significant up
regulation		 显著下调
Significant down-
regulation
苯丙烷和聚酮化合物Phenylpropane and polyketone compounds 14 10 4
苯类Benzene 3 2 1
核苷、核苷酸和类似物Nucleosides, nucleotides and analogues 4 0 4
有机氮化合物Organic nitrogen compounds 1 0 1
有机酸及其衍生物Organic acids and their derivatives 34 2 32
有机氧化合物Organic oxygen compounds 12 7 5
有机杂环化合物Organic heterocyclic compounds 10 7 3
脂质和类脂分子Lipids and lipid molecules 40 10 30
其他Others 12 4 8
合计Total 130 42 88

Fig.4

Bubble diagram of topological analysis of metabolic pathway of adzuki bean seedlings under FSA stress The bubbles in the figure represent the path; Impact value indicates the importance of the path -Log10P indicates enrichment significance"

Table 3

Enrichment of metabolic pathway in adzuki bean seedlings under FSA stress (cation)"

通路名称
Path name		 通路代谢物总数
Total pathway
metabolites		 富集代谢物个数
Number of enriched
metabolites		 P值
P-value		 Impact值
Impact
value		 代谢物名称及KEGG ID
Metabolite name and KEGG ID
嘧啶代谢
Pyrimidine metabolism		 62
 2
 0.0235
 0.1239
 尿嘧啶C00106；胞嘧啶C00380
异黄酮生物合成
Isoflavone biosynthesis		 49
 3
 0.0007
 0.1078
 2,7,4'-三羟基异黄酮C15567；甘氨酸C16195；毛蕊异黄酮C01562
嘌呤代谢
Purine metabolism		 81
 3
 0.0031
 0.0796
 鸟嘌呤C00242；次黄嘌呤C00262；β-谷甾酮C00014
半乳糖代谢
Galactose metabolism		 46
 2
 0.0136
 0.0153
 甘露三糖C05404；蔗糖C00089
精氨酸和脯氨酸代谢
Arginine and proline metabolism		 72

 3

 0.0022

 0.0073

 4-(谷氨酰胺)丁酸酯C15767；对香豆酰腐胺C18326；(2 S)-2-(3-羧基丙酰氨基)-5-氧代戊酸C05932

Table 4

Enrichment of metabolic pathway in adzuki bean seedlings under FSA stress (anion)"

通路名称
Path name		 通路代谢物总数
Total pathway
metabolites		 富集代谢物个数
Number of enriched
metabolites		 P值
P-value		 Impact值
Impact
value		 代谢物名称及KEGG ID
Metabolite name and KEGG ID
花青素生物合成
Anthocyanin biosynthesis		 41
 2
 0.0147
 0.1284
 矢车菊素-3-葡萄糖苷C12137；花葵素C05904
黄酮类生物合成
Flavonoid biosynthesis		 68 4 0.0030 0.0208 根皮苷C01604；花葵素C05904；香树脂C00974；(2 R,3 R)-3,4',7-三羟基黄烷酮C09751
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