Crops ›› 2023, Vol. 39 ›› Issue (6): 94-100.doi: 10.16035/j.issn.1001-7283.2023.06.013

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Drought Tolerance Evaluation of Soybean Mini Core Collections

Ren Honglei1,2(), Zhang Fengyi1, Han Xinchun1, Hong Huilong2, Zhu Xiao1,3, Wang Guangjin1, Qiu Lijuan2()   

  1. 1Soybean Research Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
    2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3Heilongjiang University, Harbin 150086, Heilongjiang, China
  • Received:2022-03-18 Revised:2022-08-09 Online:2023-12-15 Published:2023-12-15

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Abstract:

Drought resistance is an important ecological trait for high and stable yield of soybean with drought stress. Screening drought-tolerant germplasm resources to improve drought resistance of new varieties is one of the important ways to ensure high and stable yield of soybean. A total of 163 mini-core collections of soybean were used for drought resistance identification at soybean adult stage in Hainan province due to its dry and rainless climate featuring in winter. Four agronomic characteristics, plant height, the number of nodes, pod number per plant and seed number per plant were investigated. By using weighted membership function method and cluster analysis, total five drought resistance levels were found in 163 mini-core collections of soybean. Four accessions were identified as highly drought tolerant (level-1), namely ZDD02159, ZDD10430, ZDD13560, and ZDD16874, 14 drought resistant (level-2) germplasms, 52 moderate drought resistant (level-3) germplasms, 74 sensitive (level-4) germplasms, and 19 highly sensitive (level-5) germplasms. These research results and the excavation of drought resistant germplasms provide reference for the utilization of resources to broaden the genetic foundation and breed new drought resistant soybean varieties, as well as for the exploration and functional research of drought resistant genes.

Key words: Soybean, Mini core collection, Adult stage, Drought resistance, Comprehensive evaluation

Table 1

Description and analysis of agronomic characteristics of 163 soybean germplasm with drought stress and irrigation conditions"

性状
Trait		 灌水处理Well-water 干旱处理Water-stressed
极小值
Min.		 极大值
Max.		 均值
Mean		 SD CV 极小值
Min.		 极大值
Max.		 均值
Mean		 SD CV
株高Plant height (cm) 9.00 57.00 26.04 10.24 39.33 6.67 43.80 14.55 5.88 40.42
主茎节数Number of nodes on main stem 4.80 14.00 8.31 1.48 17.80 4.00 10.80 6.18 1.06 17.16
单株荚数Pod number per plant 4.00 123.00 26.96 16.61 61.62 2.75 39.00 13.58 7.14 52.60
单株粒数Seed number per plant 6.00 265.00 48.21 32.67 67.77 3.50 78.80 21.56 13.60 63.06

Table 2

Variance analysis of agronomic characteristics of soybean germplasms (F test)"

变异来源
Source of variation		 株高
Plant height (cm)		 主茎节数
Number of nodes on main stem		 单株荚数
Pod number per plant		 单株粒数
Seed number per plant
环境Environment 2520.38** 1556.36** 570.98** 646.44**
基因型Genotype 14.18** 6.35** 4.95** 5.34**

Table 3

Drought resistance coefficient based on different identification indexes"

参数Parameter DCPH DCNS DCPP DCSP
极小值Min. 0.2053 0.4808 0.0547 0.0437
极大值Max. 1.6846 1.2500 2.1786 2.2069
均值Mean 0.6231 0.7624 0.6396 0.5902
SD 0.3013 0.1697 0.4376 0.4612
CV 0.4836 0.2226 0.6842 0.7814

Table 4

Membership function values of 163 soybeans based on four drought resistance coefficients"

参数Parameter μ(DCPH) μ(DCNS) μ(DCPP) μ(DCSP)
均值Mean 0.2207 0.3342 0.2754 0.2106
标准差Standard deviation 0.1592 0.2014 0.2060 0.1777
CV 0.7213 0.6027 0.7481 0.8439
权重Weight 0.2474 0.2067 0.2566 0.2894

Table 5

Correlation analysis between membership function values and D-values of drought tolerance coefficient based on each index"

参数
Parameter		 μ(DCPH) μ(DCNS) μ(DCPP) μ(DCSP) D值
D-value
μ(DCPH) 1.0000
μ(DCNS) 0.8179** 1.0000
μ(DCPP) 0.5530** 0.6419** 1.0000
μ(DCSP) 0.6029** 0.6444** 0.9625** 1.0000
D值
D-value		 0.8140** 0.8581** 0.9187** 0.9309** 1.0000

Fig. 1

Cluster analysis of drought resistance of 163 soybean germplasms"

Table 6

Grading results of drought resistance of 163 soybean germplasms based on D-value"

抗旱级别
Drought
resistance grade		 D值
D-value		 材料数量
Number of
materials		 材料编号
Code
高抗
High tolerance		 0.6494~0.7365
 4
 ZDD16874、ZDD13560、ZDD02159、ZDD10430
抗
Tolerance		 0.4834~0.6074
 14
 ZDD12635、ZDD14409、ZDD14190、ZDD12680、ZDD10539、ZDD06515、ZDD02315、ZDD18277、ZDD02764、ZDD12322、ZDD12407、ZDD13233、ZDD12836、ZDD12453
中抗
Middle tolerance





 0.2442~0.4367






 52






 ZDD14911、ZDD16954、ZDD12908、ZDD18524、ZDD12331、ZDD03969、ZDD02149、ZDD13666、ZDD16675、ZDD04620、ZDD14920、ZDD17767、ZDD07610、ZDD03570、ZDD12023、ZDD15357、ZDD14783、ZDD10186、ZDD03153、ZDD17375、ZDD02990、ZDD03741、ZDD12828、ZDD02114、ZDD04429、ZDD03533、ZDD10270、ZDD03739、ZDD04572、ZDD16846、ZDD19293、ZDD08564、ZDD14505、ZDD17457、ZDD11866、ZDD03603、ZDD03733、ZDD08352、ZDD02913、ZDD02866、ZDD02940、ZDD02134、ZDD03540、ZDD10100、ZDD04604、ZDD05920、ZDD17989、ZDD19579、ZDD02891、ZDD00610、ZDD13341、ZDD08251
敏感
Susceptibility








 0.0908~0.2335









 74









 ZDD11092、ZDD16756、ZDD11586、ZDD04959、ZDD01720、ZDD11703、ZDD05502、ZDD14240、ZDD03868、ZDD14125、ZDD17574、ZDD14252、ZDD07218、ZDD06377、ZDD10812、ZDD08124、ZDD06438、ZDD11951、ZDD03293、ZDD01983、ZDD06375、ZDD04092、ZDD03776、ZDD08650、ZDD00932、ZDD05572、ZDD06363、ZDD08190、ZDD06562、ZDD08120、ZDD01489、ZDD08238、ZDD10615、ZDD02096、ZDD14782、ZDD11255、ZDD07580、ZDD20340、ZDD20387、ZDD06856、ZDD06378、ZDD19381、ZDD01060、ZDD03728、ZDD18835、ZDD18771、ZDD08633、ZDD11575、ZDD20671、ZDD06851、ZDD06803、ZDD00076、ZDD05494、ZDD18558、ZDD08728、ZDD06454、ZDD08603、ZDD00709、ZDD00059、ZDD06358、ZDD03106、ZDD00638、ZDD00046、ZDD07623、ZDD02864、ZDD00294、ZDD02892、ZDD01074、ZDD10252、ZDD01402、ZDD01629、ZDD19131、ZDD09279、ZDD00310
高敏感
High susceptibility
 0.0214~0.0878

 19

 ZDD01683、ZDD08928、ZDD08018、ZDD11226、ZDD11323、ZDD00698、ZDD01421、ZDD00326、ZDD19027、ZDD04275、ZDD06543、ZDD08228、ZDD18870、ZDD20676、ZDD21030、ZDD07370、ZDD22145、ZDD11588、ZDD22188
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