Crops ›› 2022, Vol. 38 ›› Issue (6): 70-74.doi: 10.16035/j.issn.1001-7283.2022.06.009

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Evaluation of Resistance to Soybean Mosaic Virus Strain 3 (SMV3) in Foreign Soybean Germplasm Resources

Qi Guangxun1(), Dong Lingchao1, Zhang Wei2, Yuan Cuiping1, Liu Xiaodong1, Wang Yingnan1, Dong Yingshan1, Wang Yumin1(), Zhao Hongkun1()   

  1. 1Soybean Institute, Jilin Academy of Agricultural Sciences, Changchun 130033, Jilin, China
    2Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin, China
  • Received:2022-03-29 Revised:2022-07-25 Online:2022-12-15 Published:2022-12-21
  • Contact: Wang Yumin,Zhao Hongkun E-mail:qiguangxunyl@163.com;wangym@cjaas.com;zhaohk@126.com

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Abstract:

Soybean mosaic virus is one of the major diseases endangering soybean production. In order to provide excellent resources for soybean mosaic virus strain resistance breeding in the future, 222 foreign soybean germplasm resources were identified and evaluated for resistance to SMV3 virulent strains. The results showed that the resistance variation of the tested materials to SMV3 strains was rich and the disease index was continuously distributed. The variation ranged from 12.82% to 92.59%. After two-year repeated identification, ten high resistance resources were screened, accounting for 4.51% of the tested materials, which were from Japan (4), America (3), Canada (2) and Korea (1). The ten high resistance resources, riched in genetic variation in sowing type, growth period, 100-seed weight, plant height, seed coat, hilum, flower color, pubescence color and other traits.

Key words: Soybean, Foreign germplasm resources, Soybean mosaic virus strain 3, Resistance identification

Table 1

Disease level and symptoms in resistance evaluation to soybean mosaic virus"

病情级别Disease level 症状Symptoms
0 无症状
1 轻花叶型，植株正常，叶片平展不皱，有黄绿相间的轻花叶
3 重花叶型，植株基本正常，叶片微皱有明显的黄绿相间的花叶
5 皱花叶型，植株接近正常，不矮化，叶片皱缩，有曲叶、突起
7 皱缩型，植株不正常，略矮化，叶片明显皱缩，叶片畸形卷曲
9 矮化型，植株极端矮化，叶片严重畸形卷曲，出现顶端枯死

Table 2

Evaluation criteria for soybean resistance to mosaic virus disease"

病情指数DI (%) 抗性等级Resistance grade
0≤DI≤20 高抗High resistance (HR)
20<DI≤35 抗病Resistance (R)
35<DI≤50 中抗Moderately resistance (MR)
50<DI≤70 感病Susceptible (S)
70<DI≤100 高感High susceptible (HS)

Fig.1

Disease index distributions of 222 introduced soybean germplasm resources"

Table 3

Resistance statistics to SMV3 of introduced soybean germplasm resources"

抗性级别
Resistance grade		 美国
America		 俄罗斯
Russia		 日本
Japan		 巴西
Brazil		 加拿大
Canada		 德国
Germany		 韩国
Korea		 意大利
Italy		 泰国
Thailand		 朝鲜
DPRK		 尼日利亚
Nigeria		 合计
Total
高抗HR 10 0 5 0 2 0 3 0 0 0 0 20
抗R 6 0 0 0 0 0 0 0 0 0 0 6
中抗MR 30 2 1 1 0 1 0 0 0 0 1 36
感S 110 18 11 3 3 3 1 3 2 1 0 155
高感HS 3 0 0 2 0 0 0 0 0 0 0 5
合计Total 159 20 17 6 5 4 4 3 2 1 1 222

Table 4

Disease index and characteristics of ten high resistance resources"

序号
Number		 统一编号
Accession
number		 品种
Variety		 2017年病
情指数
Disease
index in
2017 (%)		 2018年病
情指数
Disease
index in
2018 (%)		 来源
Source		 播种
类型
Sowing
type		 种皮
Seedcoat		 脐色
Hilum
color		 花色
Flower
color		 茸毛色
Pubescence
color		 株高
Plant
height
(cm)		 生育日数
Growth
duration
(d)		 百粒重
100-seed
weight
(g)
1 WDD00412 Cloud 16.05 12.08 美国 春播 黑 黑 白 灰 128.04 123 15.88
2 WDD01215 Saikai20 14.07 17.87 日本 春播 黄 褐 紫 灰 57.58 130 16.26
3 WDD01622 Epps 16.67 11.11 美国 夏播 黄 黑 紫 灰 48.68 114 12.20
4 WDD02178 Suwon123 13.73 10.58 韩国 夏播 黄 褐 紫 灰 64.62 114 16.32
5 WDD02286 关东102 17.46 9.52 日本 夏播 黄 黄 紫 灰 45.34 106 21.40
6 WDD02292 中特1号 17.65 10.37 日本 夏播 黄 黄 紫 灰 48.78 106 23.88
7 WDD02355 Yumeyutaka 18.52 9.52 日本 夏播 黄 黄 紫 灰 38.34 105 23.36
8 WDD03001 M044 14.81 16.91 美国 夏播 黄 褐 白 灰 69.34 130 13.34
9 WDD03137 CA5 12.82 1.98 加拿大 春播 黄 褐 紫 灰 112.00 126 16.38
10 WDD03156 CA26 13.13 9.44 加拿大 春播 黄 褐 紫 棕 132.06 130 15.56

Table 5

Variance analysis of high resistance resources in repeated identification"

变异来源
Source of variation		 Ⅲ型平方和
Type Ⅲ SS		 自由度
Degree of freedom		 均方
Mean square		 F值
F-value		 显著性
Significance
校正模型Corrected model 215.102a 10 21.510 1.974 0.160
截距Intercept 3492.929 1 3492.929 320.602 0.000
品种Variety 111.550 9 12.394 1.138 0.425
年份Year 103.551 1 103.551 9.505 0.013
误差Error 98.054 9 10.895
总计Total 3806.085 20
校正总计Corrected total 313.156 19
[1] Li K, Ren R, Adhimoolam K, et al. Genetic analysis and identification of two soybean mosaic virus resistance genes in soybean [Glycine max (L.) Merr]. Plant Breeding, 2015, 134:684-695.
doi: 10.1111/pbr.12315
[2] 王大刚, 陈圣男, 黄志平, 等. 193份大豆品系对SMV抗性鉴定与分子标记检测. 分子植物育种, 2019, 17(24):8138-8151.
[3] 黄志平, 李杰坤, 王维虎, 等. 大豆新品系抗SMV鉴定及其抗性来源分析. 大豆科学, 2017, 36(4):598-605.
[4] 张子戌, 张伟, 王帅, 等. 东北地区大豆种质资源SMV3号株系抗性评价及农艺性状分析. 大豆科学, 2019, 38(6):847-855.
[5] 李凯, 刘志涛, 李海朝, 等. 国家大豆区域试验品种对SMV和SCN的抗性分析. 大豆科学, 2013, 32(5):670-675.
[6] 吕文清, 张明存, 魏培文, 等. 东北三省大豆花叶病毒(SMV)株系的种类与分布. 植物病理学报, 1985, 15(4):225-229.
[7] 李凯, 夏迎春, 王大刚, 等. 黑龙江省大豆花叶病毒(SMV)株系的动态变化分析. 大豆科学, 2014, 33(6):880-884.
[8] 栾晓燕, 李宗飞, 满为群, 等. 与大豆SMV3号株系抗性相关的分子标记的鉴定. 分子植物育种, 2006, 4(6):841-845.
[9] 刘佳, 衣志刚, 董志敏, 等. 2016-2019年北方春大豆参试品种(系)花叶病和灰斑病抗性鉴定及分析. 大豆科学, 2021, 40 (1):130-141.
[10] 郑翠明, 常汝镇, 邱丽娟, 等. 大豆种质资源对SMV3号株系的抗性鉴定. 大豆科学, 2000, 19(4):299-306.
[11] 徐刚, 郜李斌, 陶波, 等. 大豆资源对大豆花叶病毒病(SMV)东北3号及黄淮7号株系的抗性研究. 东北农业大学学报, 2008, 39(10):11-14.
[12] 李文福, 刘春燕, 于妍, 等. 大豆种质资源对东北SMV1号和3号株系的抗性鉴定. 中国油料作物学报, 2009, 31(1):94-96.
[13] 李开盛, 王洪岩, 曹越平. 大豆资源对大豆花叶病毒(SMV)东北3号株系与黄淮7号株系的抗性反应. 上海交通大学学报, 2011, 29(3):53-61.
[14] 滕卫丽, 卢双勇, 高阳, 等. 不同省份大豆新品种(系)对东北大豆强弱花叶病毒株系的抗性鉴定. 东北农业大学学报, 2011, 42(10):16-20.
[15] 张伟, 刘振库, 苏前富, 等. 吉林省大豆新品种(系)抗大豆花叶病毒鉴定与评价. 吉林农业科学, 2015, 40(4):51-53,59.
[16] 高赛男, 赵雪, 李文滨, 等. 农家大豆种质对花叶病毒病N1和N3株系的抗性鉴定. 大豆科学, 2016, 35(1):117-123.
[17] 陈爱国, 王岩, 孟未来, 等. 不同原生境来源野生大豆抗花叶病毒(SMV)综合评价及聚类分析. 辽宁农业科学, 2020(1):7-13.
[18] 叶俊华, 杨启台, 刘章雄, 等. 大豆引进种质抗胞囊线虫病、抗花叶病毒病和耐盐基因型鉴定及优异等位基因聚合种质筛选. 作物学报, 2018, 44(9):1263-1273.
[19] 邱丽娟, 常汝镇, 袁翠平, 等. 国外大豆种质资源的基因挖掘利用现状与展望. 植物遗传资源学报, 2006, 7(1):1-6.
[20] 刘章雄, 常汝镇, 邱丽娟. 国家种质库保存国外大豆种质的分析研究. 植物遗传资源学报, 2009, 10(1):68-72.
[21] 邱丽娟, 常汝镇. 大豆种质资源描述规范和数据标准. 北京: 中国农业出版社, 2006:32-41.
[22] 中国农业科学院作物品种资源研究所. 中国大豆品种资源目录(续编二). 北京: 中国农业出版社, 1996:280-343.
[23] 中国农业科学院作物科学研究所. 中国大豆品种资源目录(续编三). 北京: 中国农业大学出版社, 2013:190-251.
[24] 杨崇良, 尚佑芬, 李长松, 等. 北方大豆品种资源抗大豆花叶病毒筛选. 植物病理学报, 1997(1):28-29.
[25] 唐向民, 杨守臻, 陈怀珠, 等. 160份广西春大豆种质对大豆花叶病毒株系SC15和SC18的抗性评价. 大豆科学, 2019, 38(2):181-188,197.
[26] 韩昕君, 李志辉, 傅豪, 等. 黄淮海夏大豆新品系对花叶病毒的抗性分析. 河北农业科学, 2019, 23(4):44-46.
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