Crops ›› 2025, Vol. 41 ›› Issue (6): 263-266.doi: 10.16035/j.issn.1001-7283.2025.06.033

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A Simplified and Highly Efficient Seed Production Method for Mixed Sowing and Harvesting of Rapeseed

Jiang Yingfen(), Rong Songbai, Li Cheng, Wu Xinjie, Fei Weixin, Chen Fengxiang()   

  1. Institute of Crop, Anhui Academy of Agricultural Sciences / Anhui Provincial Key Laboratory of Crop Quality Improvement, Hefei 230031, Anhui, China
  • Received:2024-08-15 Revised:2024-12-03 Online:2025-12-15 Published:2025-12-12

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Abstract:

In order to explore a simple, safe and efficient hybrid seed production strategy for rapeseed, this study used the recessive genic male sterile line 9012A as the female parent and the self-incompatible line s-29 as the male parent. The parental seeds were mixed at three ratios of 90% female parent+10% male parent, 80% female parent+20% male parent, and 75% female parent+25% male parent, and harvested at maturity. The seeds were genotyped to determine the feasibility and optimal mixing ratio of this seed production method. The results showed that the purity of hybrid seeds produced by the two mixed seeding ratios of 90% female parent+10% male parent and 80% female parent+20% male parent could meet the standards for commercial hybrid seeds. Among them, the mixed seeding ratio of 90% female parent+10% male parent had the highest yield and hybrid seed purity.

Key words: Rapeseed, Recessive genic male sterile, Self-incompatibility, Hybrid seed production, Mixed sowing

Fig.1

Polymorphic bands amplified both in parents and F1 hybrids by using SSR126"

Table 1

Yield and genotype analysis of seed production combination"

制种组合
Seed production combination		 基因型统计Genotype statistics 真杂交种率
True hybrid rate (%)		 父本基因型频率
Frequency of paternal genotype (%)		 小区收获种子量
Seed yield (kg/10 m2)
A B H
T1 4 9 81 86.2 4.2 2.13
T2 6 7 80 86.0 6.4 1.85
T3 12 5 77 82.0 12.8 1.72

Table 2

Genotype analysis of seeds from maternal parents"

制种组合
Seed production
combination		 基因型统计
Genotype statistics		 真杂交种率
True hybrid
rate (%)		 母本基因型频率
Frequency of maternal
genotype (%)		 母本收获种子量
Seed yield of maternal
parents (kg/10 m2)
A B H
T1 0 10 84 89.4 10.6 1.99
T2 0 8 86 91.5 8.5 1.65
T3 0 7 87 92.6 7.4 1.35

Table 3

Genotype analysis of seeds from paternal parents"

制种组合
Seed production
combination		 基因型统计
Genotype statistics		 真杂交种率
True hybrid
rate (%)		 自交结实率
Seed setting
rate (%)		 父本收获种子量
Seed yield of paternal
parents (kg/10 m2)
A B H
T1 50 7 37 39.4 53.2 0.14
T2 51 7 36 38.3 54.3 0.20
T3 56 4 34 36.2 59.6 0.37
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