Crops ›› 2016, Vol. 32 ›› Issue (2): 129-132.doi: 10.16035/j.issn.1001-7283.2016.02.024

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Strengthening and Rooting of Slender Potato Test-Tube Plantlet

Chang Liguo1,Fan Huiling2,Liu Jianchao1,Bai Shengwen2,Cheng Hongyu2,Li Huaqing2   

  1. 1 College of Agronomy,Northwest A & F University,Yangling 712100,Shaanxi,China;
    2 College of Agriculture and Biotechnology,Hexi University,Zhangye 734000,Gansu,China
  • Received:2015-10-14 Revised:2016-02-18 Online:2016-04-15 Published:2018-08-26
  • Contact: Huiling Fan,Jianchao Liu

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Abstract:

To probe optimized medium for strengthing and rooting of potato test-tube plantlet, using thin plants of cultivar ‘Atlantic’ as materials, medium components for strengthing of weak plantlet and concentration of auxin for rooting were optimized by single factor experiment. Results showed that 2MS and 3MS medium played a significant role in making potato test-tube seedlings strong, especially 3MS medium, in which the height of seedlings was low, stalks were strong, leaves were dark green and leaves areas were large. In addition, MS+0.5mg/L NAA was the suitable medium to induce roots for potato tube seedlings, in which roots could grow more faster, and the number of strong roots were as many as 12.39.

Key words: Potato cultivar Atlantic, MS medium, Concentration of auxin

Table 1

Effects of medium on different index parameters of potato test-tube plantlet"

性状Trait 1/4MS 1/2MS MS(CK) 2MS 3MS
株高(cm) 9.01aA 6.56bB 5.28cC 4.69dD 4.55dD
地上部鲜重(g/株) 0.19dD 0.26bB 0.22cC 0.27bB 0.37aA
单位株高重(g/cm) 0.02dD 0.04cC 0.04cC 0.06bB 0.08aA
茎节数(个/株) 5.38cC 5.38cC 5.94bB 6.12bB 6.27aA
叶片数(片/株) 7.36eD 9.32dC 10.01cC 11.76aA 10.83bB
节间距(cm) 1.32aA 0.95bB 0.82cC 0.79dCD 0.80dD
茎粗(mm) 1.28dD 1.43cC 1.57bB 2.13aA 2.17aA

Table 2

Effects of different concentration of auxin for rooting of potato test-tube plantlet"

项目Items MS+0.3mg/L NAA MS+0.5mg/L NAA MS+0.8mg/L NAA MS+1.0mg/L NAA MS(CK)
生根数(条) 10.74bB 12.39aA 2.67dD 0fF 5.63cC
根长(cm) 3.00bB 2.34cC 1.29dD 0fF 4.60aA
根重(g) 0.139bB 0.166aA 0.052dD 0fF 0.098bcC
根的其他生长情况 发根较早、锥形根 发根早、锥形根 发根迟、根极少 不发根 发根早、细长根
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