Crops ›› 2016, Vol. 32 ›› Issue (2): 133-138.doi: 10.16035/j.issn.1001-7283.2016.02.025

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Effects of Epi-Brassinosteroid on Panicle Traits of Rice during Panicle Differentiation Stage

Gong Yanlong,Xu Hai,Xia Yuanye,Du Zhimin,Shi Xiaorui,Xu Zhengjin   

  1. Rice Institute,Shenyang Agricultural University/Key Laboratory of Northeast Rice Biology and Breeding,Ministry of Agriculture/Key Laboratory of Northern Japonica Super Rice Breeding,Ministry of Education/Key Laboratory of Northern Japonica Genetics and Breeding of Liaoning Province,Shenyang 110866,Liaoning,China
  • Received:2015-11-23 Revised:2016-02-28 Online:2016-04-15 Published:2018-08-26
  • Contact: Zhengjin Xu

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Abstract:

Five rice cultivars were used as test materials to study the effects that spraying epi-brassinosteroid with lower, medium and high concentrations on the panicle traits of rice during panicle differentiation stages, which including bract differentiation stage(T1), branch differentiation stage(T2), spikelet primordium differentiation stage(T3), pollen mother cell formation and meiosis stage(T4), pollen filling stage(T5). The results were as follows, panicle length and grain number were significantly higher than that of the control. The difference on seed setting rate of primary branch was not significant, but epi-BR with medium concentrations could significantly increase seed setting rate of secondary branch, so promote the seed setting rate of whole panicle. Grains weight (include thousand grains weight of primary and secondary branch) fell significantly after applying epi-BR. Larger the concentrations, lower the seed setting rate. Grain density fell significantly after applying epi-BR, but low and medium concentrations of epi-BR were more effective on decreasing grains density than that of high concentrations. After spraying epi-BR at different periods of panicle differentiation, the effect of promoting panicle length was T4>T1>T5>T3>T2, the effect of promoting grain number was T1>T3>T2>T4>T5. The effect of promoting panicle seed setting rate was more obvious at the pollen mother cell formation and meiosis stage(T4), the effects of different spraying periods on the differences of branch number and thousand grains weight were not significant, the more delay the spraying periods, the more obvious the effect of grains density decreased. There was interaction effect between the spraying periods and concentrations. We made sure the best combination of spraying periods and concentrations aimed at regulating effect of different panicle traits.

Key words: Rice, Epi-brassinosteroid, Panicle traits, Panicle differentiation stage

Fig.1

Effect of epi-BR with different concentrations on panicle length Different small letters mean 0.05 level significant differences among treatments. The same below."

Fig.2

Effect of epi-BR with different concentrations on number of branch"

Fig.3

Effect of epi-BR with different concentrations on grains per panicle"

Fig.4

Effect of epi-BR with different concentrations on seed setting rate"

Fig.5

Effect of epi-BR with different concentrations on thousand grains weight"

Fig.6

Effect of epi-BR with different concentrations on grains density"

Fig.7

Effect of epi-BR treated at different stage on panicle length"

Fig.8

Effect of epi-BR treated at different stage on number of branch"

Fig.9

Effect of epi-BR treated at different stage on grains per panicle"

Fig.10

Effect of epi-BR treated at different stage on seed setting rate"

Fig.11

Effect of epi-BR treated at different stage on thousand grains weight"

Fig.12

Effect of epi-BR treated at different stage on grains density"

Table 1

Optimal spraying stages and concentrations of epi-BR for regulating panicle traits"

性状
Trait		 调控方向
Regulatory direction		 施用时期和浓度最佳组合
Optimal combination of spraying stage and concentration
穗长Panicle length 促进Increase T4C3
一次枝梗数Number of primary branch 促进Increase T1C2
二次枝梗数Number of secondary branch 促进Increase T1C2
穗粒数Grains per panicle 促进Increase T1C2
一次枝梗结实率Seed setting rate of primary branch 促进Increase T5C2
二次枝梗结实率Seed setting rate of secondary branch 促进Increase T4C2
整穗结实率Seed setting rate of whole panicle 促进Increase T4C2
一次枝梗千粒重Thousand grains weight of primaty branch 抑制Decrease T3C3
二次枝梗千粒重Thousand grains weight of secondary branch 抑制Decrease T4C1
总千粒重Thousand grains weight 抑制Decrease T3C3
着粒密度Grains density 抑制Decrease T5C2
[1] 徐正进, 邵国军, 韩勇 , 等. 东北三省水稻产量和品质及其与穗部性状关系的初步研究. 作物学报, 2006,32(12):1878-1883.
[2] 徐海, 朱春杰, 郭艳华 , 等. 生态环境对籼粳稻杂交后代穗部性状的影响及其与亚种特性的关系. 中国农业科学, 2009,42(5):1540-1549.
[3] 林贤青, 王雅芬, 朱德峰 , 等. 水稻茎鞘非结构性碳水化合物与穗部性状关系的研究. 中国水稻科学, 2001,15(2):155-157.
[4] Grove M D, Spencer G F, Rohwedder W K , et al. Brassinolide,a plant growth-promoting steroid isolated from Brassica napus pollen. Nature, 1979,281:216-217.
doi: 10.1038/281216a0
[5] Sasse J M . Physiological actions of brassinosteroids:an update. Journal of Plant Growth Regulation, 2003,22:276-288.
doi: 10.1007/s00344-003-0062-3
[6] 黄新, 高梦竹, 张昊 , 等. 油菜素内酯对植物细胞钙离子分布的影响. 中国农业科学, 2015,48(10):2067-2075.
[7] Rao S S R, Vardhini V B, Sujatha E , et al. Brassinosteroids-A new class of phytohormones. Current Science, 2002,82(10):1239-1245.
[8] Szekeres M, Nemeth K, Koncz-Kalman Z , et al. Brassinosteroids rescue the deficiency of CYP90,a cytochrome P450,controlling cell elongation and de-etiolatoin in Arabidopsis. Cell, 1996,85:171-182.
doi: 10.1016/S0092-8674(00)81094-6
[9] Yamamuro C, Ihara Y, Wu X , et al. Loss of function of a rice brassinosteroid insensitivel homolog prevents internode elongation and bending of the lamina joint. Plant Cell, 2000,12:1591-1606.
doi: 10.1105/tpc.12.9.1591
[10] Yu J Q, Zhou Y H, Ye S F , et al. 24-epibrassinolide and abscisic acid protect cucumber seedlings from chilling injury. Journal of Horticultural Science and Biotechnology Trustees, 2002,77(4):470-473.
doi: 10.1080/14620316.2002.11511524
[11] 曹云英, 赵华 . 高温胁迫下油菜素内酯对水稻幼苗的保护作用. 中国水稻科学, 2007,21(5):525-529.
[12] 惠竹梅, 王智真, 胡勇 , 等. 24-表油菜素内酯对低温胁迫下葡萄幼苗抗氧化系统及渗透调节物质的影响. 中国农业科学, 2013,46(5):1005-1013.
doi: 10.3864/j.issn.0578-1752.2013.05.016
[13] Núñez M, Mazzafera P, Mazorra L M , et al. Influence of a brassinosteroid analogue on antioxidant enzymes in rice grown in culture medium with NaCl. Biologia Plantarum, 2003,47(1):67-70.
[14] 康云艳, 郭世荣, 李娟 , 等. 24-表油菜素内酯对低氧胁迫下黄瓜幼苗根系抗氧化系统的影响. 中国农业科学, 2008,41(1):153-161.
[15] 康云艳, 杨暹, 郭世荣 , 等. 24-表油菜素内酯对低氧胁迫下黄瓜幼苗碳水化合物代谢的影响. 中国农业科学, 2011,44(12):2495-2503.
doi: 10.3864/j.issn.0578-1752.2011.12.011
[16] Kang Y Y, Guo S R, Li J , et al. Effect of root applied 24-epibrassinolide on carbohydrate status and fermentative enzyme activities in cucumber (Cucumis sativus L.) seedlings under hypoxia. Plant Growth Regulation, 2009,57:259-269.
doi: 10.1007/s10725-008-9344-x
[17] Haubrick L L, Assmann S M . Brassinosteroids and plant function:some clues,more puzzles.Plant, Cell and Environment, 2006,29:446-457.
doi: 10.1111/pce.2006.29.issue-3
[18] Fadzillah N M, Gill V, Finch R P , et al. Chilling,oxidative stress and antioxidant responses in shoot cultures of rice. Planta, 1996,199:552-556.
[19] Wang B K, Zeng G W . Effect of epibrassinolide on the resistance of rice seedlings to chilling injury. Journal of Plant Physiology and Molecular Biology, 1993,19:38-42.
[20] 王士银, 陈冉冉, 石庆华 , 等. 开花期外施表油菜素内酯(epi-BR)对水稻的影响.作物杂志, 2012(4):83-86.
[21] 凌启鸿, 张洪程, 苏祖芳 , 等. 水稻叶龄模式.北京: 科学出版社, 1994: 69-215.
[22] 郑飞, 于运华, 何钟佩 . 植物生长调节剂对高温胁迫下冬小麦产量形成的调控机理研究. 中国农业大学学报, 1998,3(S):7-11.
[23] 李勇, 王红光, 李瑞奇 , 等. 表油菜素内酯对冬小麦产量及氮素吸收、积累和分配的影响. 麦类作物学报, 2015,35(2):239-250.
[24] 赵长华, 丁艳锋 . 水稻穗粒数形成的生理生化研究进展.耕作与栽培, 2001(1):5-9.
[25] 华乃震 . 农药水分散粒剂的开发和进展. 现代农药, 2006,5(2):32-37.
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